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Cat. No.: HY-103068 Purity: 99.94%
Handling Instructions

Diethyl-pythiDC is an inhibitor of collagen prolyl 4-hydroxylases (CP4Hs).

For research use only. We do not sell to patients.

Diethyl-pythiDC Chemical Structure

Diethyl-pythiDC Chemical Structure

CAS No. : 1821370-70-0

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10 mM * 1  mL in DMSO USD 198 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
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10 mg USD 280 In-stock
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25 mg USD 580 In-stock
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50 mg USD 940 In-stock
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100 mg USD 1600 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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Diethyl-pythiDC is an inhibitor of collagen prolyl 4-hydroxylases (CP4Hs).

IC50 & Target


In Vitro

Diethyl-pythiDC inhibits CP4H activity in cultured cells at concentrations that do not cause iron deficiency. MDA-MB-231 cells are treated with biheteroaryl dicarboxylates, and assayed for cytotoxicity and indicators of iron deficiency. None of the esterified biheteroaryl dicarboxylates exhibited cytotoxic activity at high micromolar concentrations. Cells treated with dihydroxybenzoate (EDHB demonstrate a strong iron-deficient phenotype. In contrast, cells treated with Diethyl-pythiDC (Diethyl pythiDC) appear to be normal at concentrations as high as 500 µM. Treatment with Diethyl-pythiDC and low levels of diethyl pyimDC does not affect the level of TfR, HIF-1α, or ferritin[1].

Molecular Weight









Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (108.80 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2643 mL 16.3217 mL 32.6435 mL
5 mM 0.6529 mL 3.2643 mL 6.5287 mL
10 mM 0.3264 mL 1.6322 mL 3.2643 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (8.16 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

  • 3.

    Add each solvent one by one:  corn oil

    Solubility: 33.33 mg/mL (108.80 mM); Clear solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
Cell Assay

MDA-MB-231 cells grown in Section X are plated at a concentration of 5,000 cells/well in a clear 96-well plate. The cells are allowed to adhere for 4 h, after which the medium is removed and discarded. Fresh medium is added and the cells are treated with varying concentrations of the test compound (e.g., Diethyl-pythiDC) at 37°C for 24 h. The medium is removed, and cells are washed with Dulbecco’s PBS. The MTS reagent is added at a ratio of 1:5, and the cells are incubated at 37°C for 2 h before measuring the absorbance at 490 nm. The average absorbance is measured in triplicate for each concentration tested, and the entire experiment is repeated in duplicate. The percentage of viable cells is determined by normalizing to a PBS control (100% viable), and a H2O2 control (0% viable)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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This equation is commonly abbreviated as: C1V1 = C2V2

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