2. Metabolic Enzyme/Protease
  3. MMP
  4. Diethyl-pythiDC

Diethyl-pythiDC is an inhibitor of collagen prolyl 4-hydroxylases (CP4Hs).

For research use only. We do not sell to patients.

Diethyl-pythiDC Chemical Structure

Diethyl-pythiDC Chemical Structure

CAS No. : 1821370-70-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Diethyl-pythiDC Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Diethyl-pythiDC

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Diethyl-pythiDC is an inhibitor of collagen prolyl 4-hydroxylases (CP4Hs).

IC50 & Target

CP4H[1]
In Vitro

Diethyl-pythiDC inhibits CP4H activity in cultured cells at concentrations that do not cause iron deficiency. MDA-MB-231 cells are treated with biheteroaryl dicarboxylates, and assayed for cytotoxicity and indicators of iron deficiency. None of the esterified biheteroaryl dicarboxylates exhibited cytotoxic activity at high micromolar concentrations. Cells treated with dihydroxybenzoate (EDHB demonstrate a strong iron-deficient phenotype. In contrast, cells treated with Diethyl-pythiDC (Diethyl pythiDC) appear to be normal at concentrations as high as 500 μM. Treatment with Diethyl-pythiDC and low levels of diethyl pyimDC does not affect the level of TfR, HIF-1α, or ferritin[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Diethyl-pythiDC Related Antibodies
Molecular Weight

306.34

Formula

C14H14N2O4S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C(C2=NC=C(C(OCC)=O)S2)N=C1)OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (108.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2643 mL 16.3217 mL 32.6435 mL
5 mM 0.6529 mL 3.2643 mL 6.5287 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.16 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  Corn Oil

    Solubility: 33.33 mg/mL (108.80 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

COA (246 KB) LCMS (100 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

MDA-MB-231 cells grown in Section X are plated at a concentration of 5,000 cells/well in a clear 96-well plate. The cells are allowed to adhere for 4 h, after which the medium is removed and discarded. Fresh medium is added and the cells are treated with varying concentrations of the test compound (e.g., Diethyl-pythiDC) at 37°C for 24 h. The medium is removed, and cells are washed with Dulbecco’s PBS. The MTS reagent is added at a ratio of 1:5, and the cells are incubated at 37°C for 2 h before measuring the absorbance at 490 nm. The average absorbance is measured in triplicate for each concentration tested, and the entire experiment is repeated in duplicate. The percentage of viable cells is determined by normalizing to a PBS control (100% viable), and a H2O2 control (0% viable)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2643 mL 16.3217 mL 32.6435 mL 81.6087 mL
5 mM 0.6529 mL 3.2643 mL 6.5287 mL 16.3217 mL
10 mM 0.3264 mL 1.6322 mL 3.2643 mL 8.1609 mL
15 mM 0.2176 mL 1.0881 mL 2.1762 mL 5.4406 mL
20 mM 0.1632 mL 0.8161 mL 1.6322 mL 4.0804 mL
25 mM 0.1306 mL 0.6529 mL 1.3057 mL 3.2643 mL
30 mM 0.1088 mL 0.5441 mL 1.0881 mL 2.7203 mL
40 mM 0.0816 mL 0.4080 mL 0.8161 mL 2.0402 mL
50 mM 0.0653 mL 0.3264 mL 0.6529 mL 1.6322 mL
60 mM 0.0544 mL 0.2720 mL 0.5441 mL 1.3601 mL
80 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0201 mL
100 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8161 mL
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Diethyl-pythiDC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diethyl-pythiDC1821370-70-0MMPMatrix metalloproteinasesInhibitorinhibitorinhibit

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