2. Anti-infection
  3. Fungal Antibiotic
  4. Flucytosine

フルシトシン  (Synonyms: Flucytosine; 5-Fluorocytosine; NSC 103805; 5-フルオロシトシン; Ro 2-9915)

製品番号: HY-B0139 純度: 99.99%
COA 取扱説明書 Technical Support

Flucytosine (5-Fluorocytosine) is an antifungal compound with oral activity. Flucytosine is a widely used cytotoxic drug that, after further metabolism, produces fluorinated ribonucleotides and deoxyribonucleotides, inhibits DNA and protein synthesis, and has multiple effects such as inhibiting candida and candida neoplasm infection and producies cytotoxicity to cancer cells.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 2022-85-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
1 g $66 在庫あり
5 g $136 在庫あり
10 g   お問い合わせ  
50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Flucytosine:

フルシトシン 関連抗体

Top Publications Citing Use of Products

    Flucytosine purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2024 Jul 30:15:1450557.  [Abstract]

    We evaluated the resistance of wild-type strain SC5314 and two strains carrying ChrR trisomy and tetrasomy mutations, respectively, to a range of antifungal drugs, including azoles (clotrimazole and miconazole), echinocandins (caspofungin), and pyrimidines (5-Fluorocytosine).

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Flucytosine (5-Fluorocytosine) is an antifungal compound with oral activity. Flucytosine is a widely used cytotoxic drug that, after further metabolism, produces fluorinated ribonucleotides and deoxyribonucleotides, inhibits DNA and protein synthesis, and has multiple effects such as inhibiting candida and candida neoplasm infection and producies cytotoxicity to cancer cells[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    CCRF-CEM IC50
    >100 3
    Compound: 5-Fluorocytosine
    Cytotoxicity was determined in CEM cells, relative to RVT
    Cytotoxicity was determined in CEM cells, relative to RVT
    		[PMID: 15081000]
    CCRF-CEM IC50
    > 100 3
    Compound: 5-Fluorocytosine
    Cytotoxicity was determined in CEM cells, relative to RVT
    Cytotoxicity was determined in CEM cells, relative to RVT
    		[PMID: 15081000]
    Vero IC50
    >100 3
    Compound: 5-Fluorocytosine
    Cytotoxicity was determined in Vero cells, relative to RVT
    Cytotoxicity was determined in Vero cells, relative to RVT
    		[PMID: 15081000]
    Vero IC50
    > 100 3
    Compound: 5-Fluorocytosine
    Cytotoxicity was determined in Vero cells, relative to RVT
    Cytotoxicity was determined in Vero cells, relative to RVT
    		[PMID: 15081000]
    体外実験

    Flucytosine transfected CD-gene hMSC (CD-hMSC) effectively converts 5-FC into 5-FU, showing anti-cancer therapeutic potential[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    フルシトシン 関連抗体
    体内実験

    Flucytosine (500 mg/kg/ day, intravenous injection for 7 days) can effectively reduce tumor volume and weight in CD-hMSC mice[2].
    Flucytosine (50, 75 mg/kg/ day, gavage, 3-30 days) combines with itraconazole (HY-17514) can effectively inhibit cryptococcus infection in hamsters[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CD-hMSC mice[2]
    Dosage: 500 mg/kg
    Administration: i.v. for 7 days
    Result: Inhibited tumor volume and bodyweight.
    臨床実験
    分子量

    129.09

    分子式

    C4H4FN3O
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1NC=C(F)C(N)=N1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 16.67 mg/mL (129.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 6.67 mg/mL (51.67 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.7465 mL 38.7327 mL 77.4653 mL
    5 mM 1.5493 mL 7.7465 mL 15.4931 mL
    10 mM 0.7747 mL 3.8733 mL 7.7465 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.67 mg/mL (12.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.67 mg/mL (12.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 8.67 mg/mL (67.16 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.99%

    COA (252 KB) HNMR (117 KB) RP-HPLC (266 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 7.7465 mL 38.7327 mL 77.4653 mL 193.6633 mL
    5 mM 1.5493 mL 7.7465 mL 15.4931 mL 38.7327 mL
    10 mM 0.7747 mL 3.8733 mL 7.7465 mL 19.3663 mL
    15 mM 0.5164 mL 2.5822 mL 5.1644 mL 12.9109 mL
    20 mM 0.3873 mL 1.9366 mL 3.8733 mL 9.6832 mL
    25 mM 0.3099 mL 1.5493 mL 3.0986 mL 7.7465 mL
    30 mM 0.2582 mL 1.2911 mL 2.5822 mL 6.4554 mL
    40 mM 0.1937 mL 0.9683 mL 1.9366 mL 4.8416 mL
    50 mM 0.1549 mL 0.7747 mL 1.5493 mL 3.8733 mL
    DMSO 60 mM 0.1291 mL 0.6455 mL 1.2911 mL 3.2277 mL
    80 mM 0.0968 mL 0.4842 mL 0.9683 mL 2.4208 mL
    100 mM 0.0775 mL 0.3873 mL 0.7747 mL 1.9366 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Flucytosine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Flucytosine2022-85-75-Fluorocytosine NSC 103805 Ro 2-9915NSC103805NSC 103805NSC-103805FungalAntibiotic5-FC5-FUMKN45CD-hMSCInhibitorinhibitorinhibit

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    製品名:
    Flucytosine
    製品番号:
    HY-B0139
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