2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Protoporphyrin IX

Protoporphyrin IX  (Synonyms: プロトポルフィリンIX; Protoporphyrin IX)

製品番号: HY-B1247 純度: 98.0%
COA 取扱説明書 Technical Support

Protoporphyrin IX is a final intermediate in the heme biosynthetic pathway, which acts as a radiation sensitizer enhancing ROS generation even in a hypoxic state and inducing DNA damage. Protoporphyrin IX also acts as a photo sensitizer undergoing photobleaching that occurs through direct degradation by light irradiation. Protoporphyrin IX is formed and accumulated following 5-aminolevulinic acid (5-ALA) (HY-W000450) administration in the tumor cells of rats. Protoporphyrin IX causes selective improvement of basal cell carcinoma when activated red fluorescence of a peak wavelength at 405 nm. Protoporphyrin IX is promising for research of sonodynamic and photodynamic agents for a wide range of cancers, such as bladder cancer and nodular basal cell carcinoma.

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CAS 番号 : 553-12-8

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カスタマーレビュー

Based on 22 publication(s) in Google Scholar

Other Forms of Protoporphyrin IX:

Protoporphyrin IX 関連抗体

Top Publications Citing Use of Products

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Jun 21;22(1):358.  [Abstract]

    UV-vis spectra of TPZ, Protoporphyrin IX, PP@PLGA, TP@PLGA and PTP@PLGA.

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Genome Biol. 2022 Dec 15;23(1):259.  [Abstract]

    Protoporphyrin IX (PpIX; 0, 5, 15, 25 µM; 2 h) rapidly results in increased nuclear G4 levels in HeLa cells. Immunostaining of G4 using BG4-EGFP (green). The nuclei are stained with DAPI (blue).

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Sci Bull. 2021 May 15;66(9):925-936.  [Abstract]

    Protoporphyrin IX (for 49 h) and Verteporfin, displayed a complete suppression of viral CPE at 1.25 µmol/L and 0.31 µmol/L, respectively. Viral N protein expression in infected Vero E6 cells was assessed by immunofluorescence.

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Sci Bull. 2021 May 15;66(9):925-936.  [Abstract]

    Antiviral effect and cell cytotoxicity of Protoporphyrin IX (0-320 μmol/L, 49 h) and verteporfin. The viral RNA production in the supernatant of infected Vero E6 cells was quantified with qRT-PCR.

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Sci Bull. 2021 May 15;66(9):925-936.  [Abstract]

    Schematic presentation of treatment timing of Protoporphyrin IX and verteporfin. Briefly, Vero E6 cells were treated with Protoporphyrin IX, verteporfin or the solvent DMSO before viral infection, during viral entry and after viral entry. A total of 8 treatment groups (I–VIII) for each compound were set up.

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Sci Bull. 2021 May 15;66(9):925-936.  [Abstract]

    Blocking effect on ACE2 and SARS-CoV-2 S-mediated cell–cell fusion by Protoporphyrin IX (2.5 μmol/L, pre-treatment for one hour) and verteporfin. The inhibitory value of protoporphyrin IX or verteporfin-treated group was presented relative to that of the DMSO-treated group which was set as 100%, respectively.

    Protoporphyrin IX purchased from MedChemExpress. Usage Cited in: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2015 Jul;27(7):568-73.  [Abstract]

    To determine whether the inhibition of caveolin-1 tyrosine residues 14 ( Cav-1-Y14 ) phosphorylation with protein tyrosine kinase inhibitors ( PP2 ) will upregulate heme oxygenase-1 ( HO-1 ) activity to protect against ventilation induced lung injury in vivo of an animal model. The group D1 or D2 receive high tidal volume ventilation for 1 hour or 2 hour respectively, but they are given PP2 1 hour before high tidal volume ventilation. The groups E1 and E2 also receive high tidal volume ventilat

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

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    Human Endogenous Metabolite Microbial Metabolite

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    製品説明

    Protoporphyrin IX is a final intermediate in the heme biosynthetic pathway, which acts as a radiation sensitizer enhancing ROS generation even in a hypoxic state and inducing DNA damage. Protoporphyrin IX also acts as a photo sensitizer undergoing photobleaching that occurs through direct degradation by light irradiation. Protoporphyrin IX is formed and accumulated following 5-aminolevulinic acid (5-ALA) (HY-W000450) administration in the tumor cells of rats. Protoporphyrin IX causes selective improvement of basal cell carcinoma when activated red fluorescence of a peak wavelength at 405 nm. Protoporphyrin IX is promising for research of sonodynamic and photodynamic agents for a wide range of cancers, such as bladder cancer and nodular basal cell carcinoma[1][2][3][4][5].

    IC50 & Target

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    Bel-7402 IC50
    309.6 nM
    Compound: PPIX
    Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and subsequent 3 mW/cm2 UVA irradiation for 20 mins measured 48 hrs post irradiation by CCK8 assay
    Antiproliferative activity against human Bel7402 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and subsequent 3 mW/cm2 UVA irradiation for 20 mins measured 48 hrs post irradiation by CCK8 assay
    		[PMID: 30925340]
    体外実験

    Aminolevulinic acid (HY-W000450) (40 mg/kg, p.o., a single dose) produces full-thickness Protoporphyrin IX fluorescence in the five superficial, nodular and morpheaform basal cell carcinoma[3].
    Protoporphyrin IX (3.6 nmol/g tissue) tissue can cause necrosis in normal rat colonic mucosa on exposure to light[4].
    Protoporphyrin IX (6.6 nmol/g tumor) delays tumor growth after irradiation of a tumor in rats[4].
    Protoporphyrin IX (0-25 μM, 2 h) rapidly results in increased nuclear G4 levels in Hela cells[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Protoporphyrin IX 関連抗体

    Immunofluorescence[3]

    Cell Line: Basal cell carcinoma
    Concentration:
    Incubation Time: 4-6 h
    Result: Protoporphyrin IX fluorescence was greater at the periphery of large tumor lobules than in the center and smaller lobules fluoresced uniformly in nodular tumors.
    体内実験

    The highest level of Protoporphyrin IX (approximately 6.3 nmol/g tissue) is found in rat liver and intestine, followed by aorta (4.3 nmol/g tissue) and oesophagus (2.1 nmol/g tissue), 3 h after Aminolevulinic acid (300 mg/kg, i.v., a single dose) administration[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    562.66

    分子式

    C34H34N4O4
    CAS 番号
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    O=C(O)CCC1=C2/C=C3C(CCC(O)=O)=C(C)C(/C=C(N/4)/C(C)=C(C=C)C4=C\C5=N/C(C(C=C)=C5C)=C\C(N2)=C1C)=N/3
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 4.55 mg/mL (8.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7773 mL 8.8864 mL 17.7727 mL
    5 mM 0.3555 mL 1.7773 mL 3.5545 mL
    10 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    In Vivo Dissolution Calculator
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    純度とドキュメンテーション

    純度: 98.0%

    COA (261 KB) HNMR (289 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7773 mL 8.8864 mL 17.7727 mL 44.4318 mL
    5 mM 0.3555 mL 1.7773 mL 3.5545 mL 8.8864 mL
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    Protoporphyrin IX Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protoporphyrin IX553-12-8PPIXEndogenous Metaboliteintermediateheme biosynthetictumor cellsratsenterohepatic circulationbasal cell carcinomasonodynamicphotodynamicbladder cancerInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-B1247
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