Ticlopidine hydrochloride

Name: Ticlopidine hydrochloride
Brand: MedChemExpress
SKU: HY-B0153A
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0153A Pureza: 99.99%: Ficha de datos
COA

SDS
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Ticlopidine (PCR 5332) hydrochloride, an antithrombotic proagent, acts as an allosteric, noncompetitive inhibitor of CD39 with the IC₅₀ of 81.7 μM. Ticlopidine hydrochloride blocks several NTPDase isoenzymes with IC₅₀s of 170 μM and 149 μM for NTPDase2 and NTPDase3, respectively. Ticlopidine hydrochloride is an inhibitor of CYP2C19 human liver cytochrome. Ticlopidine hydrochloride inhibits CYP2C9 and CYP3A4 with IC₅₀s of 26.0 and 32.3 μM, respectively.

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Ticlopidine hydrochloride Estructura química

No. CAS : 53885-35-1

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 g	En stock	Estimated Time of Arrival: December 31
5 g	En stock	Estimated Time of Arrival: December 31
10 g	Obtener un presupuesto
50 g	Obtener un presupuesto

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Revisión del cliente

Based on 3 publication(s) in Google Scholar

Other Forms of Ticlopidine hydrochloride:

Ticlopidine In-stock
Ticlopidine-d₆ hydrochloride Obtener un presupuesto
Ticlopidine-d₄ hydrochloride Obtener un presupuesto
Ticlopidine hydrochloride-d₆ Obtener un presupuesto
Ticlopidine hydrochloride (Standard) Obtener un presupuesto

3 Publications Citing Use of MCE Ticlopidine hydrochloride

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas NTPDase:

Ver todas las isoformas

NTPDase2 NTPDase3 NTPDase1

Ver todos los productos específicos de isoformas Cytochrome P450:

Ver todas las isoformas

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
Platelet	IC₅₀	0.36 mM Compound: Ticlid	Antiplatelet aggregation activity in rabbit platelets assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins prior to ADP-challenge measured within 5 mins by Born's turbidimetric analysis Antiplatelet aggregation activity in rabbit platelets assessed as inhibition of ADP-induced platelet aggregation incubated for 5 mins prior to ADP-challenge measured within 5 mins by Born's turbidimetric analysis	[PMID: 23509954]

In Vitro

Ticlopidine hydrochloride exhibits activity against human CD39 with apparent K_i,app values of 14 µM^[1].
Ticlopidine hydrochloride inhibits recombinant human CD39 expressed in COS-7-cells with the K_i value of 127 µM^[1].
Growth rate is affected during the first days of culture, Ticlopidine (30 and 150 µM) hydrochloride reducing its effect in the following days^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Oral administration of Losartan (HY-17512) with 10 mg/kg Ticlopidine significantly increases the AUC (by 65.0%), suggesting that Ticlopidine can effectively inhibit the metabolism of losartan in the intestine and/or liver^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

300.25

Fòrmula

C₁₄H₁₅Cl₂NS

No. CAS

53885-35-1

Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC=CC=C1CN2CCC3=C(C=CS3)C2.Cl

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

H₂O : 50 mg/mL (166.53 mM; Need ultrasonic)

DMSO : 50 mg/mL (166.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3306 mL	16.6528 mL	33.3056 mL
5 mM	0.6661 mL	3.3306 mL	6.6611 mL
10 mM	0.3331 mL	1.6653 mL	3.3306 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (27.74 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.99%

Select Batch:

Ficha de datos (277 KB) SDS (393 KB)

COA (250 KB) LCMS (104 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Thebault JJ, et al. Effects of ticlopidine, a new platelet aggregation inhibitor in man. Clin Pharmacol Ther. 1975 Oct;18(4):485-90. [Content Brief]

[2]. Ashida SI, et al. Mode of action of ticlopidine in inhibition of platelet aggregation in the rat. Thromb Haemost. 1979 Apr 23;41(2):436-49. [Content Brief]

[3]. Laura Schäkel, et al. 2-Substituted thienotetrahydropyridine derivatives: Allosteric ectonucleotidase inhibitors. Arch Pharm (Weinheim). 2021 Dec;354(12):e2100300. [Content Brief]

[4]. I.KRASLOVA1, et al. Ticlopidine-Induced Cholestatic Inflammatory Hepatitis: New Insights into Pathogenetic Mechanisms of Drug-Related Hepatotoxicity.

[5]. Si-hyung Yang, et al. Effects of ticlopidine on pharmacokinetics of losartan and its main metabolite EXP-3174 in rats. Acta Pharmacol Sin. 2011 Jul;32(7):967-72. [Content Brief]

[6]. F Piovella, et al. The effect of Ticlopidine on human endothelial cells in culture. Thromb Res. 1984 Feb 1;33(3):323-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.3306 mL	16.6528 mL	33.3056 mL	83.2639 mL
	5 mM	0.6661 mL	3.3306 mL	6.6611 mL	16.6528 mL
	10 mM	0.3331 mL	1.6653 mL	3.3306 mL	8.3264 mL
	15 mM	0.2220 mL	1.1102 mL	2.2204 mL	5.5509 mL
	20 mM	0.1665 mL	0.8326 mL	1.6653 mL	4.1632 mL
	25 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3306 mL
	30 mM	0.1110 mL	0.5551 mL	1.1102 mL	2.7755 mL
	40 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0816 mL
	50 mM	0.0666 mL	0.3331 mL	0.6661 mL	1.6653 mL
	60 mM	0.0555 mL	0.2775 mL	0.5551 mL	1.3877 mL
	80 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
	100 mM	0.0333 mL	0.1665 mL	0.3331 mL	0.8326 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.