2. Anti-infection Cytoskeleton Metabolic Enzyme/Protease
  3. Fungal Influenza Virus Aminotransferases (Transaminases) Exosomes Bacterial Antibiotic
  4. Tunicamycin

ツニカマイシン  (Synonyms: Tunicamycin; Streptomyces lysosuperficus)

製品番号: HY-A0098 純度: 99.96%
COA 取扱説明書 Technical Support

Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity.Tunicamycin increases exosome release in cervical cancer cells.

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CAS 番号 : 11089-65-9

容量 価格（税別） 在庫状況 数量
2 mg $190 在庫あり
5 mg $385 在庫あり
10 mg $650 在庫あり
25 mg $1150 在庫あり
50 mg $2070 在庫あり
100 mg $3500 在庫あり
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カスタマーレビュー

Based on 155 publication(s) in Google Scholar

ツニカマイシン 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
Cell Proliferation/Viability Assay
Cell Imaging/Staining
Flow Cytometry

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Nat Genet. 2025 Mar;57(3):680-693.

    PD-L1 WT or mutants were transfected into SK-MEL-28 cells and treated with tunicamycin (2.5  μM) for 24 hours. PD-L1 expression was analyzed by immunoblotting (n = 3 independent experiments).

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Oct;48(5):3146-3155.  [Abstract]

    The melanocytes were treated with TM (3 μM), thapsigargin (1 μM), or DTT (1 mM) for 24 h to induce ER stress. ER stress induced an increase in the apoptosis of melanocytes.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Int J Oral Sci. 2024 Jul 1;16(1):47.  [Abstract]

    Deglycosylation of EREG enhanced the turnover of EREG. HN4 and 293-EREG cells were treated with 10 μg/mL tunicamycin for 24 h followed by pulse-chase with 100 μg/mL cycloheximide.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 May 24;9(21):eadg8156.  [Abstract]

    Cell death quantification by IncuCyte of wild-type (WT) or YFP-Parkin MEFs after 24 hours of treatment with 1 μM tunicamycin in combination or not with 40 μM qVD.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Jul:63:102765.

    SA-β-gal staining was performed MLE12 cells were treated with TM (0.25 μg/mL) for 24 hours.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Nature. 2020 Mar;579(7799):433-437.  [Abstract]

    Clonal HAP1 knockout and stably reconstituted cells were treated as indicated (CCCP, 20 μM, 9 h; Tunicamycin, HY-A0098, 10 μM, 9 h; Oligomycin (OM), 9 h; CDDO, Bardoxolone, HY-14909, 2.5 μM, 11 h; GTPP, HY-102007A, 10 μM, 11 h) and analysed by immunoblotting.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Nature. 2020 Mar;579(7799):433-437.  [Abstract]

    HAP1 CHOPNeon cells of the indicated genotypes were treated for 9 h (CCCP, 20 μM; Tunicamycin, HY-A0098, 10 μM; CDDO, Bardoxolone, HY-14909, 2.5 μM) or 12 h (GTPP, HY-102007A, 10 μM) and analysed by flow cytometry. Per genotype and treatment, the CHOPNeonsignal was normalized to its DMSO control and statistical significance is indicated compared to identically treated wild-type cells (mean ± s.d. of n = 3 independent experiments; one-way ANOVA with Dunnett’s multiple comparisons correction).

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Jun;17(6):1426-1447.  [Abstract]

    NRK cells were pre-treated with 10 μM CQ for 8 h, and followed by 0.1 μg/mL tunicamycin treatment for 6 h.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 22;9(6):604.  [Abstract]

    Western blot analysis of ER stress-related proteins after PPI treatment for 48 h in both pH 7.4 and pH 6.5 conditions. Cells treated with Thapsigargin (TG, 0.5 and 1 μM) or Tunicamycin (Tu, 2.5, 5 and 10 μg/mL) for 24 h served as positive controls.

    Tunicamycin purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2018 Oct 18;16(10):e2006483.   [Abstract]

    SK-Hep1 cells stably expressing EV or Flag-PGM1 are treated with or without 0.1 ug/mL Tunicamycin, and cell proliferation is measured.

    Aminotransferases (Transaminases) アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    GPT/ALT GOT/AST

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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    製品説明

    Tunicamycin is a mixture of homologous nucleoside antibiotic that inhibits N-linked glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes accumulation of unfolded proteins in cell endoplasmic reticulum (ER) and induces ER stress, and causes blocking of DNA synthesis and cell cycle arrest in G1 phase. Tunicamycin inhibits gram-positive bacteria, yeasts, fungi, and viruses and has anti-cancer activity[1][2][3].Tunicamycin increases exosome release in cervical cancer cells[4].

    IC50 & Target

    GPT/ALT

     

    体外実験

    Tunicamycin (2 μg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells[1].
    Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ツニカマイシン 関連抗体

    Western Blot Analysis

    Cell Line: CD44+/CD24- and original MCF7 cells[1]
    Concentration: 2 µg/mL
    Incubation Time: 24 hours
    Result: Increased level of spliced XBP-1, ATF6 nuclear translocation and CHOP protein expression are detected in CD44+/CD24- and original MCF7 cells.
    体内実験

    Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133+/- MHCC97L cells xenograft model (BALB/c (nu/nu) mice)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    844.94 (n=10)

    分子式

    C39H64N4O16
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C1NC(N([C@H]2[C@H](O)[C@H](O)[C@@H](C(C[C@@H]3[C@H](O)[C@H](O)[C@@H](NC(/C=C/[(CH2) 8-11]C(C)C)=O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O3)O)O2)C=C1)=O
    Structure Classification
    Initial Source

    Streptomyces lysosuperficus
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 33.33 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 10 mg/mL (adjust pH to 12 with 1 M NaOH)

    Ethanol : 1 mg/mL (ultrasonic and warming and heat to 60°C)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.96%

    COA (260 KB)

    取扱説明書 (2659 KB)
    参考文献
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    Tunicamycin Related Classifications

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tunicamycin11089-65-9FungalInfluenza VirusAminotransferases (Transaminases)ExosomesBacterialAntibioticAminotransferases (Transaminases) ERUDP-HexNAcantibioticglycoproteinN-linkedDNAsynthesisanti-cancerXBP-1ATF6invasionInhibitorinhibitorinhibit

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