2. Apoptosis
  3. Caspase
  4. Z-IETD-FMK

Z-IETD-FMK  (Synonyms: Z-IE(OMe)TD(OMe)-FMK)

製品番号: HY-101297 純度: 98.0%
COA 取扱説明書 Technical Support

Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor. Z-IETD-FMK is also a granzyme B inhibitor.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

カスタムペプチド

CAS 番号 : 210344-98-2

容量 価格（税別） 在庫状況 数量
1 mg $168 在庫あり
5 mg $360 在庫あり
10 mg $480 在庫あり
25 mg $790 在庫あり
50 mg $1140 在庫あり
100 mg   お問い合わせ  
200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 68 publication(s) in Google Scholar

Other Forms of Z-IETD-FMK:

Z-IETD-FMK 関連抗体

Top Publications Citing Use of Products

顧客検証

2D/3D Cell Culture and Differentiation
WB
Cell Imaging/Staining

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jun 17;16(1):452.  [Abstract]

    Representative phase-contrast images of pyroptotic cell death induced by RG7388 in H23 cells pretreated with or without pan-caspase inhibitor Z-VAD-FMK (zVAD, 50 μM), caspase-8 inhibitor Z-IETD-FMK (Cas8i, 50 μM) or caspase-3 inhibitor Z-DEVD-FMK (Cas3i, 25 μM) as indicated for 6 hours

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell. 2024 Dec 26;187(26):7533-7550.e23.  [Abstract]

    Organoids were treated with Z-IETD-FMK (Z-IETD, 40 μM, 16 h) during rIFN-λ treatment.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    THP-1 monocytes were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated for 6 h with heat-killed gram-negative bacteria at twice the number of cells. Immunoblots showing caspase-8, caspase-1 and IL-1β in total cell lysate are representative of one of two experimental repeats.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    PBMCs were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated for 16 h with heat-killed gram-negative bacteria at twice the number of monocytes.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    THP-1 monocytes were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated at a specific time point with heat-killed E. coli XL10 at twice the number of cells. Immunoblots showing P-TAK1, caspase-8 and caspase-1 in total cell lysate are representative of one of two experimental repeats.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2018 Oct:120:143-154.  [Abstract]

    Z-IETD-FMK remarkably decreases the dioscin-induced up regulation of Caspase-3

    Caspase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor[1]. Z-IETD-FMK is also a granzyme B inhibitor[5].

    IC50 & Target[1]

    Caspase-8

     

    体外実験

    T-cellZ-IETD-FMK causes full inhibition only of the proapoptotic effect of TNFα with an IC50 of 0.46 μM[1]. Z-IETD-FMK and Z-VAD-FMK at non-toxic doses are found to be immunosuppressive and inhibit human T cell proliferation induced by mitogens and IL-2. They are shown to block NF-κB in activated primary T cells, but have little inhibitory effect on the secretion of IL-2 and IFN-γ during T cell activation[2]. Z-IETD-FMK inhibits the cleavage of caspase-8 and only partially inhibits the cleavage of caspase-3 and PARP. Z-IETD-FMK can prevent the execution of apoptosis in retinal cells exposed to different apoptotic stimuli[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Z-IETD-FMK 関連抗体
    体内実験

    Pharmacological inhibition of caspase-8 by z-IETD-FMK robustly reduces tumor growth and this is closely associated with a reduction in the release of pro-inflammatory cytokines, IL-6, TNF-α, IL-18, IL-1α, IL-33, but not IL-1β. Furthermore, inhibition of caspase-8 reduces the recruitment of innate suppressive cells, such as myeloid-derived suppressor cells, but not of regulatory T cells to lungs of tumor-bearing mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    654.68

    分子式

    C30H43FN4O11
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    Sequence

    Z-Ile-Glu-Thr-Asp-FMK
    Sequence Shortening

    ZIETDFMK
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Sealed storage, away from moisture
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 41.67 mg/mL (63.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5275 mL 7.6373 mL 15.2746 mL
    5 mM 0.3055 mL 1.5275 mL 3.0549 mL
    10 mM 0.1527 mL 0.7637 mL 1.5275 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: ≥98.0%

    COA (248 KB) HNMR (244 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    T cell proliferation following mitogen stimulation is determined using [3H]-thymidine incorporation. In brief, PBMCs or purified T cells are seeded at 1×106 cells/mL in 96 well plates and stimulated with either PHA (5 μg/mL or co-stimulated with anti-CD3 mAb (5 μg/mL) and anti-CD28 mAb (2.5 μg/mL) in the presence or absence of caspase inhibitor Z-IETD-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [[3H]-labelled methyl-thymidine (0.037 MBq) prior to harvest onto glass fibre filter mats using a Tomtec automated multi-well harvester[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [4]

    Mice: Mice are divided into three groups: (1) naive, non-treated, mice; (2) CTR (control), i.t. instilled with NMU; and (3) lung cancer-bearing mice treated with Z-IETD-FMK (0.5 μg per mouse). The involvement of caspase-8 in lung cancer development is the determined at different time points (3, 7 and 28 days)[4].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5275 mL 7.6373 mL 15.2746 mL 38.1866 mL
    5 mM 0.3055 mL 1.5275 mL 3.0549 mL 7.6373 mL
    10 mM 0.1527 mL 0.7637 mL 1.5275 mL 3.8187 mL
    15 mM 0.1018 mL 0.5092 mL 1.0183 mL 2.5458 mL
    20 mM 0.0764 mL 0.3819 mL 0.7637 mL 1.9093 mL
    25 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5275 mL
    30 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
    40 mM 0.0382 mL 0.1909 mL 0.3819 mL 0.9547 mL
    50 mM 0.0305 mL 0.1527 mL 0.3055 mL 0.7637 mL
    60 mM 0.0255 mL 0.1273 mL 0.2546 mL 0.6364 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Z-IETD-FMK Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Z-IETD-FMK210344-98-2Z-IE(OMe)TD(OMe)-FMKCaspaseInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Z-IETD-FMK
    製品番号:
    HY-101297
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.