2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. TRP Channel
  4. ML-SA5

ML-SA5 is a potent TRPML1 cation channel agonist that activates the entire endosomal TRPML1 (ML1) current in DMD myocytes with an EC50 of 285 nM and is more potent than ML-SA1. ML-SA5 has anticancer activity and can inhibit tumour growth.

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CAS 番号 : 2418670-70-7

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 96 在庫あり
Solution
10 mM * 1 mL in DMSO USD 96 在庫あり
Solid
5 mg $95 在庫あり
10 mg $165 在庫あり
25 mg $345 在庫あり
50 mg $550 在庫あり
100 mg $950 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 4 publication(s) in Google Scholar

ML-SA5 関連抗体

Top Publications Citing Use of Products

    ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jun 17:10.1038/s41594-025-01581-x.  [Abstract]

    Live-cell imaging of WT expressing DMXL1–mNG and mScarlet–LAMP1 to mark lysosomes upon treatment with MLSA5. Images taken at 2-min intervals for 10 min are shown. c, As in a, but after pretreatment with BafA1 to block CASM.

    ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jun 17:10.1038/s41594-025-01581-x.  [Abstract]

    Confocal images of fixed WT U2OS cells stably expressing DMXL1-mNG treated with nocodazole (top) or pre-treated with nocodazole (5 ug/ml; 1 h) and co-treated with MLSA5 (5 μM, 2 h) (bottom). Immunostaining of LAMP1 was used to mark lysosomes (magenta). Scale bar is 10 microns; inset scale bar is 5 microns. Micrographs are from a single experiment.

    ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 May;4(5):647-663.  [Abstract]

    Representative FACS profile showing lysosomal acidity in SCLOH treated with ML-SA1, ML-SA5 (5-25 μM) and rapamycin for 24 h.

    ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 May;4(5):647-663.  [Abstract]

    Representative images of mRFP-eGFP-LC3 fluorescence change and lipid accumulation, as well as quantification analysis, in PALD SC samples after phagocytosis and with or without ML-SA1, ML-SA5 (5-25 μM; 48 h) and rapamycin treatment at the indicated concentrations (n = 14 ROIs in each group).

    TRP Channel アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    ML-SA5 is a potent TRPML1 cation channel agonist that activates the entire endosomal TRPML1 (ML1) current in DMD myocytes with an EC50 of 285 nM and is more potent than ML-SA1. ML-SA5 has anticancer activity and can inhibit tumour growth[1].
    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    1 μM
    Compound: ML-SA5
    Effect on TRPML1 knockout HEK293 cells
    Effect on TRPML1 knockout HEK293 cells
    		[PMID: 38141860]
    体外実験

    ML-SA5(1-100 μM, 24 h) has some cell-targeting specificity and induces substantial cell death in M12 and MeWo cells, but fully preserves normal melanocytes. It also causes a loss of mitochondrial membrane potential in M12 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML-SA5 関連抗体
    体内実験

    ML-SA5 (i.p., 2-5 mg/kg, daily, 2 weeks) reduces muscle necrosis in MDX mice by more than 70% and reduces central nucleated fibers, suggesting that ML-SA5 can improve muscle atrophy in mdx mice in vivo by promoting myosin repair, but has no effect in ML1 knockout mice. Moreover, ML-SA5 reduces skeletal and cardiac muscle damage in mdx mice through ML1 upregulation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    457.99

    分子式

    C19H24ClN3O4S2
    CAS 番号
    Appearance

    Solid

    Color

    White to light brown

    SMILES

    O=S(NC1=C(N2CCCCC2)C(Cl)=CC=C1)(C3=CC=C(S(=O)(N(C)C)=O)C=C3)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (218.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1835 mL 10.9173 mL 21.8345 mL
    5 mM 0.4367 mL 2.1835 mL 4.3669 mL
    10 mM 0.2183 mL 1.0917 mL 2.1835 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.69%

    COA (255 KB) HNMR (263 KB) RP-HPLC (246 KB) MS (178 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1835 mL 10.9173 mL 21.8345 mL 54.5863 mL
    5 mM 0.4367 mL 2.1835 mL 4.3669 mL 10.9173 mL
    10 mM 0.2183 mL 1.0917 mL 2.1835 mL 5.4586 mL
    15 mM 0.1456 mL 0.7278 mL 1.4556 mL 3.6391 mL
    20 mM 0.1092 mL 0.5459 mL 1.0917 mL 2.7293 mL
    25 mM 0.0873 mL 0.4367 mL 0.8734 mL 2.1835 mL
    30 mM 0.0728 mL 0.3639 mL 0.7278 mL 1.8195 mL
    40 mM 0.0546 mL 0.2729 mL 0.5459 mL 1.3647 mL
    50 mM 0.0437 mL 0.2183 mL 0.4367 mL 1.0917 mL
    60 mM 0.0364 mL 0.1820 mL 0.3639 mL 0.9098 mL
    80 mM 0.0273 mL 0.1365 mL 0.2729 mL 0.6823 mL
    100 mM 0.0218 mL 0.1092 mL 0.2183 mL 0.5459 mL
    • No file chosen (Maximum size is: 1024 Kb)
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    ML-SA5 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML-SA52418670-70-7TRP ChannelTransient receptor potential channelsTRPML1ML1DMD myocytesanticancerML-SA1Inhibitorinhibitorinhibit

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    製品名:
    ML-SA5
    製品番号:
    HY-152182
    数量:
    MCE 日本正規代理店:

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