2,5-Dihydroxybenzoic acid (Synonyms: Gentisic acid)

Name: 2,5-Dihydroxybenzoic acid
Brand: MedChemExpress
SKU: HY-W001179
Rating: 5.0 (2 reviews)

Cat. No.: HY-W001179 Pureza: 99.92%: Ficha de datos
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Instrucciones de manejo
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2,5-Dihydroxybenzoic acid is a derivative of benzoic and a powerful inhibitor of fibroblast growth factors.

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2,5-Dihydroxybenzoic acid Estructura química

No. CAS : 490-79-9

Tamaño	Stock	Cantidad

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
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Revisión del cliente

Based on 2 publication(s) in Google Scholar

Other Forms of 2,5-Dihydroxybenzoic acid:

2,5-Dihydroxybenzoic acid (Standard) In-stock

2 Publications Citing Use of MCE 2,5-Dihydroxybenzoic acid

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Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

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Referencias
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Descripciòn

2,5-Dihydroxybenzoic acid is a derivative of benzoic and a powerful inhibitor of fibroblast growth factors.

IC₅₀ & Target^[2]

Microbial Metabolite

Fibroblast growth factor

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	>100 μM Compound: c	Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader	[PMID: 21745700]
HEK293	IC₅₀	>15 mM Compound: 13	Cytotoxicity against HEK293 cells harboring pendrin P123S mutant after 72 hrs by MTT assay Cytotoxicity against HEK293 cells harboring pendrin P123S mutant after 72 hrs by MTT assay	[PMID: 28341401]
J774	IC₅₀	>256 μM Compound: 8	Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by resazurin assay Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by resazurin assay	[PMID: 25884114]

In Vitro

2,5-Dihydroxybenzoic acid (Gentisic acid) is a derivative of benzoic and a minor product of the metabolic break down of aspirin^[1]. 2,5-Dihydroxybenzoic acid is also a component of many traditional liquors and herbal remedies, is singled out as a powerful inhibitor of fibroblast growth factors. 2,5-Dihydroxybenzoic acid is used as a lead to identify additional compounds with better inhibitory characteristics generating a new chemical class of fibroblast growth factor inhibitors that includes the agent responsible for alkaptonuria. Through low and high resolution approaches, using representative members of the fibroblast growth factor family and their cell receptors, it is shown that this class of inhibitors may employ two different mechanisms to interfere with the assembly of the signaling complexes that trigger fibroblast growth factor-driven mitogenesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

It is verified from in vivo disease models that this group of inhibitors (e.g., 2,5-Dihydroxybenzoic acid) may be of interest to treat cancer and angiogenesis-dependent diseases^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

154.12

Fòrmula

C₇H₆O₄

No. CAS

490-79-9

Appearance

Solid

Color

White to off-white

SMILES

OC(C=C1)=C(C(O)=O)C=C1O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : ≥ 100 mg/mL (648.85 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 7.14 mg/mL (46.33 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.4885 mL	32.4423 mL	64.8845 mL
5 mM	1.2977 mL	6.4885 mL	12.9769 mL
10 mM	0.6488 mL	3.2442 mL	6.4885 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.97%

Select Batch:

Ficha de datos (278 KB) SDS (392 KB)

COA (252 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Levy G, et al. Salicylate accumulation kinetics in man. N Engl J Med. 1972 Aug 31;287(9):430-2. [Content Brief]

[2]. IS Fernández, et al. Gentisic acid, a compound associated with plant defense and a metabolite of aspirin, heads a new class of in vivo fibroblast growth factor inhibitors. Journal of Biological Chemistry , 2010, 285(15):11714-29. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.4885 mL	32.4423 mL	64.8845 mL	162.2113 mL
	5 mM	1.2977 mL	6.4885 mL	12.9769 mL	32.4423 mL
	10 mM	0.6488 mL	3.2442 mL	6.4885 mL	16.2211 mL
	15 mM	0.4326 mL	2.1628 mL	4.3256 mL	10.8141 mL
	20 mM	0.3244 mL	1.6221 mL	3.2442 mL	8.1106 mL
	25 mM	0.2595 mL	1.2977 mL	2.5954 mL	6.4885 mL
	30 mM	0.2163 mL	1.0814 mL	2.1628 mL	5.4070 mL
	40 mM	0.1622 mL	0.8111 mL	1.6221 mL	4.0553 mL
DMSO	50 mM	0.1298 mL	0.6488 mL	1.2977 mL	3.2442 mL
	60 mM	0.1081 mL	0.5407 mL	1.0814 mL	2.7035 mL
	80 mM	0.0811 mL	0.4055 mL	0.8111 mL	2.0276 mL
	100 mM	0.0649 mL	0.3244 mL	0.6488 mL	1.6221 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2,5-Dihydroxybenzoic acid Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2,5-Dihydroxybenzoic acid490-79-9Gentisic acidEndogenous MetaboliteFGFRFibroblast growth factor receptorInhibitorinhibitorinhibit

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2,5-Dihydroxybenzoic acid (Synonyms: Gentisic acid)

Revisión del cliente

Other Forms of 2,5-Dihydroxybenzoic acid:

2,5-Dihydroxybenzoic acid Related Antibodies

2 Publications Citing Use of MCE 2,5-Dihydroxybenzoic acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

2,5-Dihydroxybenzoic acid Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

2,5-Dihydroxybenzoic acid Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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