Pyridoxal 5'-phosphate hydrate  (Synonyms: Pyridoxal phosphate hydrate)

Pyridoxal 5'-phosphate hydrate, the active form of vitamin B6, is an essential cofactor for multiple enzymes, including aromatic l-amino acid decarboxylase that catalyzes the final stage in the production of the neurotransmitters dopamine and serotonin. Pyridoxal 5'-phosphate hydrate is the most important coenzyme variant in the process of vitamin B6 intracellular phosphorylation and is interconvertible with other variants, including pyridoxine 5′‐phosphate (PNP) and pyridoxamine 5′-phosphate (PMP)^[1][2].

Pyridoxal 5'-phosphate hydrate (500 μM, 24 h) activates the ERK/c-Jun signaling pathway, promotes the expression of IGFBP1 protein in HepG2 cells^[3].
Pyridoxal 5'-phosphate hydrate (0.5 mg/mL, 48h) inhibits the proliferation of cancer cells SK-OV-3 and OVCAR-3^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyridoxal 5'-phosphate hydrate (1 mg/kg, ip, single dose) alleviates Aβ25-35-induced spatial memory impairment and long-term memory impairment in mouse models^[4].
Pyridoxal 5'-phosphate hydrate (600 mg/kg/day, po for 16 weeks) inhibits the formation of advanced glycation end-products (AGEs), and prevents the progression of diabetic nephropathy in Streptozotocin (HY-13753)-induced rats diabetes model^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

247.14

C₈H₁₀NO₆P

853645-22-4

Solid

Off-white to light yellow

O=CC1=C(O)C(C)=NC=C1COP(O)(O)=O.[x H2O]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Allen GF, et al. Pyridoxal 5'-phosphate deficiency causes a loss of aromatic L-amino acid decarboxylase in patients and human neuroblastoma cells, implications for aromatic L-amino acid decarboxylase and vitamin B(6) deficiency states.J Neurochem. 2010 Jul;114(1):87-96.  [Content Brief]

  [2]. Calderón-Ospina CA, et al. B Vitamins in the nervous system: Current knowledge of the biochemical modes of action and synergies of thiamine, pyridoxine, and cobalamin.CNS Neurosci Ther. 2020 Jan;26(1):5-13.  [Content Brief]

  [3]. Zhang P, et al., High concentrations of pyridoxal stimulate the expression of IGFBP1 in HepG2 cells through upregulation of the ERK/c‑Jun pathway. Mol Med Rep. 2013 Oct;8(4):973-8.  [Content Brief]

  [4]. Choi JM, et al., Membrane-Free Stem Cells and Pyridoxal 5'-Phosphate Synergistically Enhance Cognitive Function in Alzheimer's Disease Mouse Model. Antioxidants (Basel). 2022 Mar 21;11(3):601.  [Content Brief]

  [5]. Zhang L, et al., Pyridoxine 5'-phosphate oxidase is a novel therapeutic target and regulated by the TGF-β signalling pathway in epithelial ovarian cancer. Cell Death Dis. 2017 Dec 13;8(12):3214.  [Content Brief]

  [6]. Nakamura S, et al., Pyridoxal phosphate prevents progression of diabetic nephropathy. Nephrol Dial Transplant. 2007 Aug;22(8):2165-74.  [Content Brief]