2. Metabolic Enzyme/Protease Neuronal Signaling Stem Cell/Wnt MAPK/ERK Pathway
  3. Endogenous Metabolite Amyloid-β ERK
  4. Pyridoxal 5'-​phosphate monohydrate

Pyridoxal 5'-​phosphate monohydrate  (Synonyms: Pyridoxal phosphate monohydrate)

Cat. No.: HY-W011727A Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

Pyridoxal 5'-phosphate monohydrate, the active form of vitamin B6, is an essential cofactor for multiple enzymes, including aromatic l-amino acid decarboxylase that catalyzes the final stage in the production of the neurotransmitters dopamine and serotonin. Pyridoxal 5'-phosphate monohydrate is the most important coenzyme variant in the process of vitamin B6 intracellular phosphorylation and is interconvertible with other variants, including pyridoxine 5′‐phosphate (PNP) and pyridoxamine 5′‐phosphate (PMP).

For research use only. We do not sell to patients.

CAS No. : 41468-25-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 g In-stock
5 g In-stock
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Based on 4 publication(s) in Google Scholar

Other Forms of Pyridoxal 5'-​phosphate monohydrate:

Pyridoxal 5'-​phosphate monohydrate Related Antibodies

Top Publications Citing Use of Products

    Pyridoxal 5'-​phosphate monohydrate purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2025 Oct 15;73(41):25991-25997.  [Abstract]

    Schematic diagram of the active site of DmDDC (PDB code: 3K40). Following the structural alignment of DmDDC and hDDC, the ligand PLP (Pyridoxal phosphate)-DME (L-dopa methyl ester) of hDDC was displayed in DmDDC. PLPDME, an intermediate in the reaction between PLP and L-dopa, is shown in green in the figure.

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    Human Endogenous Metabolite Microbial Metabolite

    View All ERK Isoform Specific Products:

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    ERK ERK1 ERK2 ERK5 ERK8

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pyridoxal 5'-phosphate monohydrate, the active form of vitamin B6, is an essential cofactor for multiple enzymes, including aromatic l-amino acid decarboxylase that catalyzes the final stage in the production of the neurotransmitters dopamine and serotonin. Pyridoxal 5'-phosphate monohydrate is the most important coenzyme variant in the process of vitamin B6 intracellular phosphorylation and is interconvertible with other variants, including pyridoxine 5′‐phosphate (PNP) and pyridoxamine 5′‐phosphate (PMP)[1][2].

    IC50 & Target

    IC50: active form of vitamin B6[1]
    In Vitro

    Pyridoxal 5'-phosphate monohydrate (500 μM, 24 h) activates the ERK/c-Jun signaling pathway, promotes the expression of IGFBP1 protein in HepG2 cells[3].
    Pyridoxal 5'-phosphate monohydrate (0.5 mg/mL, 48h) inhibits the proliferation of cancer cells SK-OV-3 and OVCAR-3[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pyridoxal 5'-​phosphate monohydrate Related Antibodies

    Western Blot Analysis[3]

    Cell Line: HepG2
    Concentration: 500 μM
    Incubation Time: 24 h
    Result: Promoted the phosphorylation of ERK1 and c-Jun protein.

    Cell Proliferation Assay[5]

    Cell Line: SK-OV-3 and OVCAR-3
    Concentration: 0.5 mg/mL
    Incubation Time: 48 h
    Result: Inhibited the proliferation.
    In Vivo

    Pyridoxal 5'-phosphate monohydrate (1 mg/kg, ip, single dose) alleviates Aβ25-35-induced spatial memory impairment and long-term memory impairment in mouse models[4].
    Pyridoxal 5'-phosphate monohydrate (600 mg/kg/day, po for 16 weeks) inhibits the formation of advanced glycation end-products (AGEs), and prevents the progression of diabetic nephropathy in Streptozotocin (HY-13753)-induced rats diabetes model[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Aβ25-35-induced cognitive dysfunction in ICR mouse models[4]
    Dosage: 1 mg/kg
    Administration: ip, single dose
    Result: Alleviated spatial and long-term memory impairment.
    Animal Model: Streptozotocin (HY-13753)-induced rats diabetes model[6]
    Dosage: 600 mg/kg
    Administration: po for 16 weeks
    Result: Prevented the diabetes.
    Molecular Weight

    265.16

    Formula

    C8H12NO7P
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=CC1=C(O)C(C)=NC=C1COP(O)(O)=O.[H]O[H]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 6.25 mg/mL (23.57 mM; Need ultrasonic)

    DMSO : 5 mg/mL (18.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7713 mL 18.8565 mL 37.7131 mL
    5 mM 0.7543 mL 3.7713 mL 7.5426 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.5 mg/mL (1.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.5 mg/mL (1.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.0%

    SDS (394 KB)

    COA (249 KB) HNMR (261 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.7713 mL 18.8565 mL 37.7131 mL 94.2827 mL
    5 mM 0.7543 mL 3.7713 mL 7.5426 mL 18.8565 mL
    10 mM 0.3771 mL 1.8857 mL 3.7713 mL 9.4283 mL
    15 mM 0.2514 mL 1.2571 mL 2.5142 mL 6.2855 mL
    H2O 20 mM 0.1886 mL 0.9428 mL 1.8857 mL 4.7141 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Pyridoxal 5'-​phosphate monohydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pyridoxal 5'-​phosphate41468-25-1Pyridoxal phosphateEndogenous MetaboliteAmyloid-βERKβ-amyloid peptideAβAbetaExtracellular signal regulated kinasesCoenzymevariantvitamin B6PLPPNPInhibitorinhibitorinhibit

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