2. Anti-infection Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis Bacterial DNA Stain Antibiotic
  4. 7-Aminoactinomycin D

7-Aminoactinomycin D (7-AAD) a cell-impermeant fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.

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7-Aminoactinomycin D

7-Aminoactinomycin D Estructura química

No. CAS : 7240-37-1

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Revisión del cliente

Based on 8 publication(s) in Google Scholar

Other Forms of 7-Aminoactinomycin D:

7-Aminoactinomycin D Related Antibodies

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Descripciòn

7-Aminoactinomycin D (7-AAD) a cell-impermeant fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects[1][2][3].

IC50 & Target

RNA Polymerase

 

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-10 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 0.5-5 µM with PBS or serum-free medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Fix cells.
2.2 Pellet and resuspend cells.
2.3 Add 7-Aminoactinomycin D (7-AAD) staining solution (0.5 to 5 µM).
2.4 Incubate for 15 to 60 minutes.
2.5 Measure fluorescence intensity (Ex/Em = 549/648 nm).

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry[1].
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Aminoactinomycin D Related Antibodies
Peso molecular

1270.43

Fòrmula

C62H87N13O16
No. CAS
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

648
Excitation (Ex)

549
SMILES

O=C(N[C@H](C(N1[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]2C)=O)=O)=O)([H])CCC1)=O)C(C)C)[C@H]2NC(C(C(C(OC3=C(C)C(N)=C4)=C5C)=NC3=C4C(N[C@@H]6C(N[C@H](C(N7[C@@](C(N(C)CC(N(C)[C@@H](C(C)C)C(O[C@@H]6C)=O)=O)=O)([H])CCC7)=O)C(C)C)=O)=O)=C(N)C5=O)=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvente y solubilidad
In Vitro: 

DMSO : 100 mg/mL (78.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7871 mL 3.9357 mL 7.8714 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: ≥99.0%

SDS (584 KB)

COA (263 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7871 mL 3.9357 mL 7.8714 mL 19.6784 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL 3.9357 mL
10 mM 0.0787 mL 0.3936 mL 0.7871 mL 1.9678 mL
15 mM 0.0525 mL 0.2624 mL 0.5248 mL 1.3119 mL
20 mM 0.0394 mL 0.1968 mL 0.3936 mL 0.9839 mL
25 mM 0.0315 mL 0.1574 mL 0.3149 mL 0.7871 mL
30 mM 0.0262 mL 0.1312 mL 0.2624 mL 0.6559 mL
40 mM 0.0197 mL 0.0984 mL 0.1968 mL 0.4920 mL
50 mM 0.0157 mL 0.0787 mL 0.1574 mL 0.3936 mL
60 mM 0.0131 mL 0.0656 mL 0.1312 mL 0.3280 mL
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7-Aminoactinomycin D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Aminoactinomycin D7240-37-17-AADDNA/RNA SynthesisBacterialDNA StainAntibioticRNApolymeraseDNAstainantibacterialflowcytometryInhibitorinhibitorinhibit

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Nombre del producto:
7-Aminoactinomycin D
Cat. No.:
HY-D1020
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