2. Vitamin D Related/Nuclear Receptor Anti-infection Apoptosis
  3. Estrogen Receptor/ERR HBV Bcl-2 Family
  4. Estradiol benzoate

安息香酸エストラジオール  (Synonyms: Estradiol benzoate; β-Estradiol 3-benzoate; 17β-Estradiol 3-benzoate)

製品番号: HY-B1192 純度: 99.90%
COA 取扱説明書 Technical Support

Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice.

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CAS 番号 : 50-50-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
1 g $66 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of Estradiol benzoate:

安息香酸エストラジオール 関連抗体

Top Publications Citing Use of Products

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227.  [Abstract]

    Estradiol benzoate (0.3 μg; i.m.;every three days). H&E staining of the vehicle group revealed endometrial epithelial cells and stromal cells within the endometriotic lesions, along with glandular hyperplasia in the lamina propria.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227.  [Abstract]

    Morphology of endometriotic lesions excised from the peritoneum of mice. Estradiol benzoate (0.3 μg; i.m.;every three days) successfully induced EMS in mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15387-15410.  [Abstract]

    Estradiol benzoate (17β-Estradiol) (1 μM; drinking water) was used to sustain the MCF7 xenograft model in NCG mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 8;21(1):81.  [Abstract]

    Estradiol benzoate (3 μg) induced endometriosis-like lesions in diverse peritoneal locations of female mice.

    Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2019 Dec 5:245:112181.  [Abstract]

    ICR female mice were treated with Estradiol benzoate (1 mg/kg; i.p.; 3 d). On the fourth day, the mice were injected with oxytocin (50 U/kg) to induce dysmenorrhea.

    Estrogen Receptor/ERR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

    Bcl-2 Family アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice[1][2][3][4][5].

    IC50 & Target

    Bax

     

    体外実験

    Estradiol benzoate (5-75 μM, 24 h-48 h) has a downregulation effect on HBV translation, transcription, and replication in SBHX21 cells[3].
    Estradiol benzoate (0-500 μM, 72 h) has toxicity to Human foreskin fibroblasts (HFFs)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    安息香酸エストラジオール 関連抗体

    Cell Cytotoxicity Assay[4]

    Cell Line: HFFs
    Concentration: 0-500 μM
    Incubation Time: 72 h
    Result: Inhibited HFFs at the 50% toxicity of 34.11 μM.
    体内実験

    Estradiol benzoate (20-100 μg/kg, Subcutaneous injection, once a day for 4-5 weeks) improves learning and memory behavior of Ovx female mice[1].
    Estradiol benzoate (0.015-15000 μg/kg, Subcutaneous injection, single dose) advances male puberty in male Sprague-Dawley (SD) rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ovx mice[1]
    Dosage: 20, 100 or 200 μg/kg
    Administration: Subcutaneous injection (s.c.)
    Result: Significantly reversed the lowered uterine weight induced by Ovx.
    Had no obvious effects on locomotor activity in Ovx mice.
    Effectively reversed the changes of enlarging the width of synaptic cleft and reducing the thickness of PSD induced by Ovx.
    Increased the number and density of synaptic vesicles by 4-week replacement with EB (100 μg/kg).
    分子量

    376.49

    分子式

    C25H28O3
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@@H](O)CC[C@@]1([H])[C@]3([H])CCC4=C(C=CC(OC(C5=CC=CC=C5)=O)=C4)[C@@]3([H])CC2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (132.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 3.33 mg/mL (8.84 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6561 mL 13.2806 mL 26.5611 mL
    5 mM 0.5312 mL 2.6561 mL 5.3122 mL
    10 mM 0.2656 mL 1.3281 mL 2.6561 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.90%

    COA (249 KB) HNMR (139 KB) RP-HPLC (255 KB) MS (67 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.6561 mL 13.2806 mL 26.5611 mL 66.4028 mL
    5 mM 0.5312 mL 2.6561 mL 5.3122 mL 13.2806 mL
    DMSO 10 mM 0.2656 mL 1.3281 mL 2.6561 mL 6.6403 mL
    15 mM 0.1771 mL 0.8854 mL 1.7707 mL 4.4269 mL
    20 mM 0.1328 mL 0.6640 mL 1.3281 mL 3.3201 mL
    25 mM 0.1062 mL 0.5312 mL 1.0624 mL 2.6561 mL
    30 mM 0.0885 mL 0.4427 mL 0.8854 mL 2.2134 mL
    40 mM 0.0664 mL 0.3320 mL 0.6640 mL 1.6601 mL
    50 mM 0.0531 mL 0.2656 mL 0.5312 mL 1.3281 mL
    60 mM 0.0443 mL 0.2213 mL 0.4427 mL 1.1067 mL
    80 mM 0.0332 mL 0.1660 mL 0.3320 mL 0.8300 mL
    100 mM 0.0266 mL 0.1328 mL 0.2656 mL 0.6640 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
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    Estradiol benzoate Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Estradiol50-50-0β-Estradiol 3-benzoate 17β-Estradiol 3-benzoateEstrogen Receptor/ERRHBVBcl-2 FamilyHepatitis B virusOvx miceHepG2Huh7SBHX21HepG2.2.15Human foreskin fibroblasts (HFFs)Inhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-B1192
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