2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. EAAT iGluR
  4. Evans Blue

エバンスブルー  (Synonyms: Evans Blue; Direct Blue 53; ダイレクトブル- 53)

製品番号: HY-B1102 純度: 99.86%
COA 取扱説明書 Technical Support

Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 314-13-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
100 mg $25 在庫あり
500 mg $35 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 29 publication(s) in Google Scholar

Other Forms of Evans Blue:

エバンスブルー 関連抗体

Top Publications Citing Use of Products

顧客検証

In Vivo Efficacy Study
IF

    Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106.  [Abstract]

    Representative images of injured spinal cord in the control, ZFgf11a, and MFGF11b groups after Evans Blue (EB, 2 mL/kg, i.v.; single dose) dye injection at 4 dpi.

    Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106.  [Abstract]

    Representative immunofluorescence images of Evans Blue (EB, 2 mL/kg, i.v.; single dose) extravasation at the lesion epicenter in the transverse sections of the spinal cord in the control, ZFgf11a, and MFGF11b groups at 4 dpi.

    Evans Blue purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Jul 12;74(8):268.  [Abstract]

    Representative images of Evans blue (4 ml/kg; i.v.; single dose) leakage and the difference between TGF-β3 and PBS groups (n = 5).

    Evans Blue purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Jan;13(3):e2302320.  [Abstract]

    The effects of different groups (control, CLP, Ca-MOF, Zolif, CMZ, RCMZ, and γ3-RCMZ) on CLP-induced vascular permeability were examined by measuring Evans blue dye in peritoneal washings. Evans blue (10 mg/kg; i.v.; single dose) injection revealed that γ3-RCMZ effectively reduced endothelial leakage levels to one-sixth of those observed in the CLP group, indicating its predominant antiendothelial leakage effect in sepsis.

    Evans Blue purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 Jun 26;13(11):3761-3780.  [Abstract]

    Sufu-cKO and WT mice were intraperitoneally injected with PBS or LPS (5 mg/kg) for 23 h, then injected with Evans blue dye (30 mg/kg body weight; i.v.; single dose) for an additional 40 min before mice were killed and lung tissues harvested. Means ± SEM (n = 5).

    EAAT アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    EAAT1 EAAT2 EAAT3 EAAT4 EAAT5

    iGluR アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    AMPA Receptor NMDA Receptor Kainate Receptor

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    Sf9 IC50
    0.922 μM
    Compound: 20, NSC-8680
    Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis
    Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis
    		[PMID: 20349977]
    体外実験

    Evans blue, a bis-azo dye, is a non-permeating dye. In presence of plasma membrane damage, Evans blue enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    エバンスブルー 関連抗体
    体内実験

    The amount of Evans Blue (2% solution; 4 mL/kg) stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CD-1 mice bearing intracerebral hemorrhage (ICH)[2]
    Dosage: 2% solution; 4 mL/kg
    Administration: Jugular vein or intraperitoneal injection; once
    Result: Showed the amount of stain accumulated in the brains.
    臨床実験
    分子量

    960.81

    分子式

    C34H24N6Na4O14S4
    CAS 番号
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    CC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(C)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 31.25 mg/mL (32.52 mM; Need ultrasonic)

    DMSO : ≥ 25 mg/mL (26.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0408 mL 5.2039 mL 10.4079 mL
    5 mM 0.2082 mL 1.0408 mL 2.0816 mL
    10 mM 0.1041 mL 0.5204 mL 1.0408 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.86%

    COA (250 KB) HNMR (354 KB) RP-HPLC (230 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.0408 mL 5.2039 mL 10.4079 mL 26.0197 mL
    5 mM 0.2082 mL 1.0408 mL 2.0816 mL 5.2039 mL
    10 mM 0.1041 mL 0.5204 mL 1.0408 mL 2.6020 mL
    15 mM 0.0694 mL 0.3469 mL 0.6939 mL 1.7346 mL
    20 mM 0.0520 mL 0.2602 mL 0.5204 mL 1.3010 mL
    25 mM 0.0416 mL 0.2082 mL 0.4163 mL 1.0408 mL
    H2O 30 mM 0.0347 mL 0.1735 mL 0.3469 mL 0.8673 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Evans Blue Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Evans Blue314-13-6Direct Blue 53 T-1824 C.I. 23860Direct Blue53Direct Blue-53T1824T 1824T-1824EAATiGluRExcitatory amino acid transporter Glutamate transporterIonotropic glutamate receptorsInhibitorinhibitorinhibit

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    製品名:
    Evans Blue
    製品番号:
    HY-B1102
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