2. Membrane Transporter/Ion Channel PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway
  3. Piezo Channel Akt ERK Potassium Channel
  4. Yoda 1

Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation.

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CAS 番号 : 448947-81-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 118 在庫あり
Solution
10 mM * 1 mL in DMSO USD 118 在庫あり
Solid
1 mg $51 在庫あり
5 mg $108 在庫あり
10 mg $144 在庫あり
25 mg $350 在庫あり
50 mg $576 在庫あり
100 mg $1020 在庫あり
250 mg $1500 在庫あり
500 mg $2100 在庫あり
1 g $2950 在庫あり
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カスタマーレビュー

Based on 100 publication(s) in Google Scholar

Yoda 1 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Imaging/Staining
IF
WB
Cell Proliferation/Viability Assay

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620.  [Abstract]

    Pre-treatment with Dooku1 slowed the rate of spheroid formation.

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Dec 3:36:102620.  [Abstract]

    IF staining confirmed the on-target activity of Dooku1, as it significantly suppressed the Yoda1 (0.5 μM)-induced upregulation of Piezo1 itself

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Sep 11:e2409081.  [Abstract]

    Expression levels of Piezo1/integrin β1/YAP signal in T24 cells exposed to Yoda1 or GsMTx4.

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 7:76:103346.  [Abstract]

    Protein levels of GPX4 and LOX in the control, OGD/R-induced, BAPN-treated OGD/R, and BAPN combined with Yoda1 (5 nM) treatment groups of HT22 cells.

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Small Sci. 2024 Jun 30;4(9):2400061.  [Abstract]

    Cell viability curve of Yoda1 (0.5-16 μM; MSCs)

    Yoda 1 purchased from MedChemExpress. Usage Cited in: Small Sci. 2024 Jun 30;4(9):2400061.  [Abstract]

    Western blot images of CaMKII, p-CaMKII, ERK, and p-Eek in rMSCs after 0–60 min 5 μm Yoda1 treatment.

    Yoda 1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 Aug:62:105-117.  [Abstract]

    Western blot of p-PI3K, PI3K, p-AKT and AKT in OA HACs treated with si-PIEZO1 for 48 h.

    Akt アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Akt Akt1 Akt2 Akt3

    ERK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ERK ERK1 ERK2 ERK5 ERK8

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation[1][2][3].

    IC50 & Target

    ERK1

     

    ERK2

     

    体外実験

    Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1[2].
    Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Yoda 1 関連抗体

    Western Blot Analysis

    Cell Line: human coronary artery endothelial cells (HCAECs)[2], A431 cells[3]
    Concentration: 0, 1.5, 3.0, and 6.0 μM
    Incubation Time: 5 min
    Result: Induced the activation of both Akt and ERK1/2, and increased the phosphorylation levels of both Akt and ERK1/2 in a dose-dependent manner. Inhibited EGF-induced increase in the amount of Rac1-GTP, and inhibited Rac1 activation
    分子量

    355.27

    分子式

    C13H8Cl2N4S2
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 15.62 mg/mL (43.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 5 mg/mL (14.07 mM; Need ultrasonic)

    Methanol : 2.5 mg/mL (7.04 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8148 mL 14.0738 mL 28.1476 mL
    5 mM 0.5630 mL 2.8148 mL 5.6295 mL
    10 mM 0.2815 mL 1.4074 mL 2.8148 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.56 mg/mL (4.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 1.56 mg/mL (4.39 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL Corn oil, and mix evenly.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  15% Cremophor EL    85% Saline

      Solubility: 0.62 mg/mL (1.75 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.97%

    COA (250 KB) HNMR (257 KB) RP-HPLC (244 KB) LCMS (97 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Methanol / Ethanol / DMSO 1 mM 2.8148 mL 14.0738 mL 28.1476 mL 70.3690 mL
    5 mM 0.5630 mL 2.8148 mL 5.6295 mL 14.0738 mL
    Ethanol / DMSO 10 mM 0.2815 mL 1.4074 mL 2.8148 mL 7.0369 mL
    DMSO 15 mM 0.1877 mL 0.9383 mL 1.8765 mL 4.6913 mL
    20 mM 0.1407 mL 0.7037 mL 1.4074 mL 3.5185 mL
    25 mM 0.1126 mL 0.5630 mL 1.1259 mL 2.8148 mL
    30 mM 0.0938 mL 0.4691 mL 0.9383 mL 2.3456 mL
    40 mM 0.0704 mL 0.3518 mL 0.7037 mL 1.7592 mL
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    Yoda 1 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Yoda 1448947-81-7Yoda1Yoda-1Piezo ChannelAktERKPotassium ChannelPKBProtein kinase BExtracellular signal regulated kinasesKcsAA431 cellsHCAECsPiezo1Endothelial cellsERK1/2Inhibitorinhibitorinhibit

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    製品名:
    Yoda 1
    製品番号:
    HY-18723
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