Guanine

Name: Guanine
Brand: MedChemExpress
SKU: HY-Y1055
Rating: 5.0 (3 reviews)

Art. -Nr.: HY-Y1055 Reinheit: 98.0%: Data Sheet
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Guanine is one of the fundamental components of nucleic acids (DNA and RNA). Guanine is a purine derivative, consisting of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine has the potential to serve as a large-capacity N pool. Guanine has cytotoxic, antinociceptive and neuroprotective effects.

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Guanine Chemische Struktur

CAS. Nr. : 73-40-5

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Based on 3 publication(s) in Google Scholar

Other Forms of Guanine:

Guanine-¹³C In-stock
Guanine-¹³C,¹⁵N₂ In-stock
Guanine-¹⁵N₅ In-stock
Guanine-¹³C₅ In-stock
Guanine hydrochloride In-stock
Guanine (Standard) In-stock
Guanine-¹³C₂,¹⁵N Angebot einholen
Guanine-¹³C₂,7-¹⁵N Angebot einholen
Guanine-¹³C₅,¹⁵N₅ Angebot einholen
Guanine-d₅ Angebot einholen

3 Publications Citing Use of MCE Guanine

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Human Endogenous Metabolite Microbial Metabolite

Biologische Aktivität
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Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Microbial Metabolite

Fungal Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	> 100 μg/mL Compound: guanine	Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting Inhibition of phosphatidylinositol 4-kinase in human A431 cell membrane by liquid scintillation counting	[PMID: 1666120]
CCRF-CEM	IC₅₀	64.3 μM Compound: Guanine	Cytotoxicity was determined in CEM cells, relative to RVT Cytotoxicity was determined in CEM cells, relative to RVT	[PMID: 15081000]
Vero	CC₅₀	136.5 μM Compound: R7	Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay	[PMID: 27933947]
Vero	IC₅₀	11.5 μM Compound: R7	Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay Inhibition of Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells assessed as inhibition of Stx-induced cytotoxicity pre-treated with compound for 1 hr followed by Stx exposure for 24 hrs by neutral red uptake assay	[PMID: 27933947]
Vero	IC₅₀	54.9 μM Compound: Guanine	Cytotoxicity was determined in Vero cells, relative to RVT Cytotoxicity was determined in Vero cells, relative to RVT	[PMID: 15081000]

In Vitro

Guanine (1 h) inhibits Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells, with IC₅₀ of 11.5 μM^[2].
Guanine (24 h) shows cytotoxicity against African green monkey Vero cells, with CC₅₀ of 136.5 μM^[2].
Guanine (10 μM, 16-40 h) partially antagonizes the cytostatic effect of Mycophenolic acid (HY-B0421) on LAN5 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Guanine (600 nmol; i.t.) inhibits spinal glutamate- and AMPA-induced biting behaviour in mice^[4].
Guanine (200-600 nmol; i.t.) produces A1 adenosine receptor-mediated antinociception against intraplantar glutamate-, Capsaicin (HY-10448)-, and Acetic acid (HY-Y0319)-induced pain in mice, with ID50 values of 107, 84, and 48 nmol and maximal inhibitions of 48%, 58%, and 56%, respectively^[4].
Guanine (200-600 nmol; i.t.) produces dose-dependent antinociception against tail-flick test thermal nociception in mice, with peak efficacy at 400 nmol yielding ~78% MPE^[4].
Guanine (200-600 nmol; i.t.) dose-dependently increases CSF levels of inosine, xanthine, and uric acid, with a significant increase in AMP at the highest dose^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Swiss albino (male, 3-4 months of age, 30-40 g)^[4]
Dosage:	200 nmol; 400 nmol; 600 nmol
Administration:	i.t.
Result:	Produced dose-dependent antinociception with maximal inhibition of 48% (ID₅₀ = 107 nmol), 58% (ID₅₀ = 84 nmol), and 56% (ID₅₀ = 48 nmol); 200-600 nmol elicited significant MPE (56%-78%). Dose-dependently elevated CSF inosine (200 nmol: 5.12 μM; 400 nmol: 11.43 μM; 600 nmol: 13.60 μM), AMP (600 nmol: 1.49 μM), xanthine (400 nmol: 7.20 μM; 600 nmol: 8.38 μM) and uric acid (200 nmol: 6.85 μM; 400 nmol: 6.57 μM; 600 nmol: 5.54 μM) levels.

Animal Model:	Swiss albino (male, 3-4 months of age, 30-40 g)^[4]
Dosage:	600 nmol
Administration:	i.t.
Result:	Significantly reduced the duration of biting behaviour induced by spinal glutamate. Significantly reduced the duration of biting behaviour induced by spinal AMPA.

Molekulargewicht

151.13

Formel

C₅H₅N₅O

CAS. Nr.

73-40-5

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(N)NC2=C1NC=N2

Structure Classification

Others

Initial Source

Microorganisms

Endogenous metabolite

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

0.5 M NaOH : 10 mg/mL (66.17 mM; ultrasonic and adjust pH to 12 with NaOH)

DMSO : 0.15 mg/mL (0.99 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6168 mL	33.0841 mL	66.1682 mL
5 mM	1.3234 mL	6.6168 mL	13.2336 mL
10 mM	0.6617 mL	3.3084 mL	6.6168 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Reinheit & Dokumentation

Purity: 98.0%

Select Batch:

Data Sheet (280 KB) SDS (394 KB)

COA (249 KB) HNMR (245 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Mojzeš P, et al. Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32722-32730. [Content Brief]

[2]. Chauhan V, et al. In Silico Discovery and Validation of Amide Based Small Molecule Targeting the Enzymatic Site of Shiga Toxin. J Med Chem. 2016 Dec 8;59(23):10763-10773. [Content Brief]

[3]. Messina E, et al. Guanine nucleotide depletion triggers cell cycle arrest and apoptosis in human neuroblastoma cell lines. Int J Cancer. 2004 Mar 1;108(6):812-7. [Content Brief]

[4]. de Oliveira ED, et al. Mechanisms involved in the antinociception induced by spinal administration of inosine or guanine in mice. Eur J Pharmacol. 2016 Feb 5;772:71-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

0.5 M NaOH	1 mM	6.6168 mL	33.0841 mL	66.1682 mL	165.4205 mL
	5 mM	1.3234 mL	6.6168 mL	13.2336 mL	33.0841 mL
	10 mM	0.6617 mL	3.3084 mL	6.6168 mL	16.5420 mL
	15 mM	0.4411 mL	2.2056 mL	4.4112 mL	11.0280 mL
	20 mM	0.3308 mL	1.6542 mL	3.3084 mL	8.2710 mL
	25 mM	0.2647 mL	1.3234 mL	2.6467 mL	6.6168 mL
	30 mM	0.2206 mL	1.1028 mL	2.2056 mL	5.5140 mL
	40 mM	0.1654 mL	0.8271 mL	1.6542 mL	4.1355 mL
	50 mM	0.1323 mL	0.6617 mL	1.3234 mL	3.3084 mL
	60 mM	0.1103 mL	0.5514 mL	1.1028 mL	2.7570 mL

Guanine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guanine73-40-5DNA/RNA SynthesisEndogenous MetaboliteDNARNAnucleic acidspurinederivativeN poolInhibitorinhibitorinhibit

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Guanine

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Other Forms of Guanine:

Guanine Related Antibodies

3 Publications Citing Use of MCE Guanine

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen:

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Guanine Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Guanine Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

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SAFETY DATA SHEET (SDS)

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