Guanine hydrochloride

Name: Guanine hydrochloride
Brand: MedChemExpress
SKU: HY-Y1055A
Rating: 5.0 (3 reviews)

Cat. No.: HY-Y1055A Purity: 98.78%: Data Sheet
COA

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Guanine hydrochloride is one of the fundamental components of nucleic acids (DNA and RNA). Guanine hydrochloride is a purine derivative, consisting of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine hydrochloride has the potential to serve as a large-capacity N pool. Guanine hydrochloride has cytotoxic, antinociceptive and neuroprotective effects.

For research use only. We do not sell to patients.

Guanine hydrochloride Chemical Structure

CAS No. : 635-39-2

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Guanine hydrochloride:

Guanine In-stock

3 Publications Citing Use of MCE Guanine hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Human Endogenous Metabolite

Microbial Metabolite

Fungal Metabolite

In Vitro

Guanine (1 h) hydrochloride inhibits Shigella dysenteriae type 1 Shiga toxin A subunit in African green monkey Vero cells, with IC₅₀ of 11.5 μM^[2].

Guanine (24 h) hydrochloride shows cytotoxicity against African green monkey Vero cells, with CC₅₀ of 136.5 μM^[2].
Guanine (10 μM, 16-40 h) hydrochloride partially antagonizes the cytostatic effect of Mycophenolic acid (HY-B0421) on LAN5 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Guanine (600 nmol; i.t.) hydrochloride inhibits spinal glutamate- and AMPA-induced biting behaviour in mice^[4].
Guanine (200-600 nmol; i.t.) hydrochloride produces A1 adenosine receptor-mediated antinociception against intraplantar glutamate-, Capsaicin (HY-10448)-, and Acetic acid (HY-Y0319)-induced pain in mice, with ID50 values of 107, 84, and 48 nmol and maximal inhibitions of 48%, 58%, and 56%, respectively^[4].
Guanine (200-600 nmol; i.t.) hydrochloride produces dose-dependent antinociception against tail-flick test thermal nociception in mice, with peak efficacy at 400 nmol yielding ~78% MPE^[4].
Guanine (200-600 nmol; i.t.) hydrochloride dose-dependently increases CSF levels of inosine, xanthine, and uric acid, with a significant increase in AMP at the highest dose^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Swiss albino (male, 3-4 months of age, 30-40 g)^[4]
Dosage:	200 nmol; 400 nmol; 600 nmol
Administration:	i.t.
Result:	Produced dose-dependent antinociception with maximal inhibition of 48% (ID₅₀ = 107 nmol), 58% (ID₅₀ = 84 nmol), and 56% (ID₅₀ = 48 nmol); 200-600 nmol elicited significant MPE (56%-78%). Dose-dependently elevated CSF inosine (200 nmol: 5.12 μM; 400 nmol: 11.43 μM; 600 nmol: 13.60 μM), AMP (600 nmol: 1.49 μM), xanthine (400 nmol: 7.20 μM; 600 nmol: 8.38 μM) and uric acid (200 nmol: 6.85 μM; 400 nmol: 6.57 μM; 600 nmol: 5.54 μM) levels.

Animal Model:	Swiss albino (male, 3-4 months of age, 30-40 g)^[4]
Dosage:	600 nmol
Administration:	i.t.
Result:	Significantly reduced the duration of biting behaviour induced by spinal glutamate. Significantly reduced the duration of biting behaviour induced by spinal AMPA.

Molecular Weight

187.59

Formula

C₅H₆ClN₅O

CAS No.

635-39-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(N)NC2=C1NC=N2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (266.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3308 mL	26.6539 mL	53.3077 mL
5 mM	1.0662 mL	5.3308 mL	10.6615 mL
10 mM	0.5331 mL	2.6654 mL	5.3308 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.78%

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Data Sheet (281 KB) SDS (394 KB)

COA (251 KB) HNMR (247 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Mojzeš P, et al. Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc Natl Acad Sci U S A. 2020;117(51):32722-32730. [Content Brief]

[2]. Chauhan V, et al. In Silico Discovery and Validation of Amide Based Small Molecule Targeting the Enzymatic Site of Shiga Toxin. J Med Chem. 2016;59(23):10763-10773. [Content Brief]

[3]. Messina E, et al. Guanine nucleotide depletion triggers cell cycle arrest and apoptosis in human neuroblastoma cell lines. Int J Cancer. 2004;108(6):812-817. [Content Brief]

[4]. de Oliveira ED, et al. Mechanisms involved in the antinociception induced by spinal administration of inosine or guanine in mice. Eur J Pharmacol. 2016;772:71-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.3308 mL	26.6539 mL	53.3077 mL	133.2694 mL
	5 mM	1.0662 mL	5.3308 mL	10.6615 mL	26.6539 mL
	10 mM	0.5331 mL	2.6654 mL	5.3308 mL	13.3269 mL
	15 mM	0.3554 mL	1.7769 mL	3.5538 mL	8.8846 mL
	20 mM	0.2665 mL	1.3327 mL	2.6654 mL	6.6635 mL
	25 mM	0.2132 mL	1.0662 mL	2.1323 mL	5.3308 mL
	30 mM	0.1777 mL	0.8885 mL	1.7769 mL	4.4423 mL
	40 mM	0.1333 mL	0.6663 mL	1.3327 mL	3.3317 mL
	50 mM	0.1066 mL	0.5331 mL	1.0662 mL	2.6654 mL
	60 mM	0.0888 mL	0.4442 mL	0.8885 mL	2.2212 mL
	80 mM	0.0666 mL	0.3332 mL	0.6663 mL	1.6659 mL
	100 mM	0.0533 mL	0.2665 mL	0.5331 mL	1.3327 mL