Cat. No.: HY-D0186

2'-Deoxyuridine

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

2’-deoxyuridine is a brain-penetrant pyrimidines nucleotide that is associated with nervous system diseases. 2'-Deoxyuridine could increase chromosome breakage and results in a decreased thymidylate synthetase activity. 2'-Deoxyuridine is a precursor in the synthesis of Edoxudine (HY-B1011) and also an analogue of 5-ethynyl-2'-deoxyuridine, EdU (HY-118411). 2’-deoxyuridine reduces microglial activation and improve oxidative stress damage by modulating glycolytic metabolism on the Aβ25-35-induced brain injury, which is promising for research of Alzheimer’s disease (AD).

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 951-78-0
Formula: C₉H₁₂N₂O₅
Molecular Weight:228.20

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

2'-Deoxyuridine Chemical Structure

CAS No.: 951-78-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-7	CC₅₀	>200 μg/mL Compound: 4	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 20863701]
Vero	IC₅₀	2.2 nM Compound: 2'-Deoxyuridine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]

In Vitro

The interaction between the 2-deoxyuridine and the column increases the duration of retention of 2-deoxyuridine^[2].
Gradient elution with sodium acetate buffer-ACN eluent on two ZIC-HILIC homemade columns separates 2-deoxyuridine in under 9 min^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2'-Deoxyuridine (34.42 ng/mL, gavage, 15 min) passes the blood-brain barrier (BBB) to enter the hippocampus of mice brain^[3].
2'-Deoxyuridine (20 mg/kg, gavage, daily for 4 weeks) improves cognition and memory loss and attenuates the damage to the hippocampus in Aβ25-35-induced mice model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Kunming mice (KM, males, aged 7 weeks, 38–42 g)^[3]
Dosage:

34.42 ng/mL
Administration:

gavage, 15 min
Result:

The concentration of 2'-Deoxyuridine in the hippocampus of mice brain treated with 2'-Deoxyuridine was higher than the control and model groups.

Animal Model:

Aβ25-35-induced mice model^[3]
Dosage:

20 mg/kg
Administration:

gavage, daily for 4 weeks
Result:

Improved cognition and memory loss and attenuated the damage to the hippocampus in Aβ25-35-induced mice model.

Chemical Information

CAS No. 951-78-0
Appearance Solid
Molecular Weight 228.20
Formula C₉H₁₂N₂O₅
Color White to off-white
SMILES

O=C(N1)N([C@H]2C[C@H](O)[C@@H](CO)O2)C=CC1=O
Structure Classification
- Others
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Journal Impact Factor

Mol Med Rep

Causal association between metabolites and upper gastrointestinal tumors: A Mendelian randomization study. [Abstract]

2024 Dec;30(6):212. PMID: 39370813
PLoS Genet

A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]

2026 Jan 23;22(1):e1012013. PMID: 41575937
J Pharm Biomed Anal

A novel UHPLC-MS/MS approach for simultaneous quantification of pyrimidine metabolites in human biofluids. [Abstract]

2025 Nov 15:265:117026. PMID: 40544701

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (438.21 mM; Need ultrasonic)

DMSO : 100 mg/mL (438.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3821 mL

21.9106 mL

43.8212 mL

5 mM

0.8764 mL

4.3821 mL

8.7642 mL

10 mM

0.4382 mL

2.1911 mL

4.3821 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (438.21 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Reidy JA, et al. Deoxyuridine increases folate-sensitive fragile site expression in human lymphocytes. Am J Med Genet. 1987 Jan;26(1):1-5. [Content Brief]

[2]. Ashraf Saad Rasheed, et al. Development of ZIC-HILIC methods using ultraviolet detection for determining 2-deoxyuridine in human serum[J]. Systematic Reviews in Pharmacy. 2020 March; 11(2):388-394.

[3]. Liu M, et al. Thymidine and 2'-deoxyuridine reduce microglial activation and improve oxidative stress damage by modulating glycolytic metabolism on the Aβ25-35-induced brain injury[J]. Arch Biochem Biophys. 2022 Oct 30;729:109377. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.3821 mL	21.9106 mL	43.8212 mL	109.5530 mL
	5 mM	0.8764 mL	4.3821 mL	8.7642 mL	21.9106 mL
	10 mM	0.4382 mL	2.1911 mL	4.3821 mL	10.9553 mL
	15 mM	0.2921 mL	1.4607 mL	2.9214 mL	7.3035 mL
	20 mM	0.2191 mL	1.0955 mL	2.1911 mL	5.4777 mL
	25 mM	0.1753 mL	0.8764 mL	1.7528 mL	4.3821 mL
	30 mM	0.1461 mL	0.7304 mL	1.4607 mL	3.6518 mL
	40 mM	0.1096 mL	0.5478 mL	1.0955 mL	2.7388 mL
	50 mM	0.0876 mL	0.4382 mL	0.8764 mL	2.1911 mL
	60 mM	0.0730 mL	0.3652 mL	0.7304 mL	1.8259 mL
	80 mM	0.0548 mL	0.2739 mL	0.5478 mL	1.3694 mL
	100 mM	0.0438 mL	0.2191 mL	0.4382 mL	1.0955 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

2'-Deoxyuridine

3 Cited Publications

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2'-Deoxyuridine

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Publications Citing Use of MedChemExpress (MCE) 2'-Deoxyuridine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2'-Deoxyuridine Related Classifications

HY-D0186 Related Classifications