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  3. 5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2'-deoxyuridine 

Cat. No.: HY-118411 Purity: 99.68%
Handling Instructions

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

5-Ethynyl-2'-deoxyuridine Chemical Structure

5-Ethynyl-2'-deoxyuridine Chemical Structure

CAS No. : 61135-33-9

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Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 45 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 45 In-stock
Estimated Time of Arrival: December 31
Solid
50 mg USD 41 In-stock
Estimated Time of Arrival: December 31
100 mg USD 72 In-stock
Estimated Time of Arrival: December 31
250 mg USD 143 In-stock
Estimated Time of Arrival: December 31
500 mg USD 264 In-stock
Estimated Time of Arrival: December 31
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5 g   Get quote  

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Based on 4 publication(s) in Google Scholar

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Description

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation[1]. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs[2].

IC50 & Target

Alkyl-Chain

 

In Vitro

5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[1].
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two month old female mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Two month old female mice[1]
Dosage: 10, 20, 50, 100 or 200 mg/kg
Administration: Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result: EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.
Molecular Weight

252.22

Formula

C11H12N2O5

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (198.24 mM; Need ultrasonic)

H2O : 25 mg/mL (99.12 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9648 mL 19.8240 mL 39.6479 mL
5 mM 0.7930 mL 3.9648 mL 7.9296 mL
10 mM 0.3965 mL 1.9824 mL 3.9648 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.25 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (8.25 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.44 mg/mL (5.71 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.5 mg/mL (1.98 mM); Clear solution

  • 5.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (1.98 mM); Clear solution

  • 6.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 0.5 mg/mL (1.98 mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: 99.77%

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Product Name:
5-Ethynyl-2'-deoxyuridine
Cat. No.:
HY-118411
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