5-Ethynyl-2'-deoxyuridine (Synonyms: EdU)

Name: 5-Ethynyl-2'-deoxyuridine
Brand: MedChemExpress
SKU: HY-118411
Rating: 5.0 (32 reviews)

Cat. No.: HY-118411 Purity: 99.78%: Data Sheet
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5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

CAS No. : 61135-33-9

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Based on 32 publication(s) in Google Scholar

Other Forms of 5-Ethynyl-2'-deoxyuridine:

5-Ethynyl-2'-deoxyuridine-¹³C Get quote
5-Ethynyl-2'-deoxyuridine-¹³C₂ Get quote

32 Publications Citing Use of MCE 5-Ethynyl-2'-deoxyuridine

Histological Imaging/Staining

: 5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]; Ki67 and EdU staining of distal jejunum of mice treated. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. P. g: Live P. goldsteinii. Red/white dotted line marks single intestinal crypt. Ten random points of one sample were picked and the average value for each mouse was calculated as one single point. Red: EdU; Blue: DAPI. n=10 replicates/group.

: 5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]; EdU (upper set) and TUNEL (bottom set) staining of organoids after being cocultured with HPG or LPG. White dotted line marks a single organoid. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. Scale bars: 20 μm. Red: EdU; Green: TUNEL; Blue: DAPI. n=20 biological replicates/group.

: 5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2024 Feb 14;32(2):191-208.e9. [Abstract]; Statistical analysis of EdU (Left) and TUNEL (right) staining of organoids generated from mice treated. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-20-deoxyuridine at 100 mg/kg was intraperitoneally injected into mice 12 h prior to sacrifice. n=20 biological replicates/group.

: 5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]; Representative Edu and Olfm4 staining and quantitation in intestine of CA-treated colitic mice. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-2’-deoxyuridine (Edu) at 100 mg/kg was intraperitoneally injected into mice 2 h prior to killing.

: 5-Ethynyl-2'-deoxyuridine purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]; Representative Muc2 or Lyz with Edu staining of intestine sections. To measure the proliferation of intestinal epithelial cells, 5-ethynyl-2’-deoxyuridine (Edu) at 100 mg/kg was intraperitoneally injected into mice 2 h prior to killing.

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Biological Activity
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Description

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation^[1]. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[2]. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

Alkyl-Chain

Cellular Effect

Cell Line	Type	Value	Description	References
E6SM	EC₅₀	0.1 μg/mL Compound: 7, EDU	Antiviral activity against Vaccinia virus in E6SM cells assessed as reduction of virus-induced cytopathogenicity Antiviral activity against Vaccinia virus in E6SM cells assessed as reduction of virus-induced cytopathogenicity	[PMID: 17518459]
E6SM	EC₅₀	0.7 μg/mL Compound: 7, EDU	Antiviral activity against HSV1 KOS in E6SM cells assessed as reduction of virus-induced cytopathogenicity Antiviral activity against HSV1 KOS in E6SM cells assessed as reduction of virus-induced cytopathogenicity	[PMID: 17518459]
E6SM	EC₅₀	0.7 μg/mL Compound: 7, EDU	Antiviral activity against thymidine kinase-deficient HSV1 B2006 in E6SM cells assessed as reduction of virus-induced cytopathogenicity Antiviral activity against thymidine kinase-deficient HSV1 B2006 in E6SM cells assessed as reduction of virus-induced cytopathogenicity	[PMID: 17518459]
E6SM	EC₅₀	2 μg/mL Compound: 7, EDU	Antiviral activity against HSV2 G in E6SM cells assessed as reduction of virus-induced cytopathogenicity Antiviral activity against HSV2 G in E6SM cells assessed as reduction of virus-induced cytopathogenicity	[PMID: 17518459]
E6SM	EC₅₀	4 μg/mL Compound: 7, EDU	Antiviral activity against thymidine kinase deficient HSV1 VMW1837 in E6SM cells assessed as reduction of virus-induced cytopathogenicity Antiviral activity against thymidine kinase deficient HSV1 VMW1837 in E6SM cells assessed as reduction of virus-induced cytopathogenicity	[PMID: 17518459]
FM3A	IC₅₀	0.13 μM Compound: 7, EDU	Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV1 thymidine kinase Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV1 thymidine kinase	[PMID: 17518459]
FM3A	IC₅₀	0.39 μM Compound: 7, EDU	Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV2 thymidine kinase Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells expressing HSV2 thymidine kinase	[PMID: 17518459]
FM3A	IC₅₀	>= 250 μM Compound: 7, EDU	Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells Inhibition of proliferation of thymidine kinase deficient mouse FM3A cells	[PMID: 17518459]
HEL	CC₅₀	2.5 μM Compound: 7, EDU	Cytotoxicity against HEL cells Cytotoxicity against HEL cells	[PMID: 17518459]
HEL	EC₅₀	0.11 μM Compound: 7, EDU	Antiviral activity against TK+ VZV YS in HEL cells assessed as reduction of virus plaque formation Antiviral activity against TK+ VZV YS in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
HEL	EC₅₀	0.16 μM Compound: 7, EDU	Antiviral activity against TK+ VZV OKA in HEL cells assessed as reduction of virus plaque formation Antiviral activity against TK+ VZV OKA in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
HEL	EC₅₀	0.75 μM Compound: 7, EDU	Antiviral activity against thymidine kinase deficient VZV YS in HEL cells assessed as reduction of virus plaque formation Antiviral activity against thymidine kinase deficient VZV YS in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
HEL	EC₅₀	0.81 μM Compound: 7, EDU	Antiviral activity against thymidine kinase deficient VZV 07/1 in HEL cells assessed as reduction of virus plaque formation Antiviral activity against thymidine kinase deficient VZV 07/1 in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
HEL	EC₅₀	0.85 μM Compound: 7, EDU	Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation Antiviral activity against HCMV Davis in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
HEL	EC₅₀	1.2 μM Compound: 7, EDU	Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation Antiviral activity against HCMV AD169 in HEL cells assessed as reduction of virus plaque formation	[PMID: 17518459]
MCF7	IC₅₀	0.4 μM Compound: 2h	Cytotoxicity against human MCF7 cells after 72 hrs Cytotoxicity against human MCF7 cells after 72 hrs	[PMID: 17336074]
MDA-MB-231	IC₅₀	4.4 μM Compound: 2h	Cytotoxicity against human MDA-MB-231 cells after 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs	[PMID: 17336074]
OST	IC₅₀	23 μM Compound: 7, EDU	Inhibition of proliferation of thymidine kinase deficient OST cells Inhibition of proliferation of thymidine kinase deficient OST cells	[PMID: 17518459]
S49	EC₅₀	0.12 μM Compound: EdUrd	Effective concentration to inhibit the growth of Wild-type S49 cells Effective concentration to inhibit the growth of Wild-type S49 cells	[PMID: 6796687]

In Vitro

5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two-month old female mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Two month old female mice^[1]
Dosage:	10, 20, 50, 100 or 200 mg/kg
Administration:	Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result:	EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.

Clinical Trial

Molecular Weight

252.23

Formula

C₁₁H₁₂N₂O₅

CAS No.

61135-33-9

Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1C[C@H](N2C(NC(C(C#C)=C2)=O)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (198.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (99.12 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9646 mL	19.8232 mL	39.6464 mL
5 mM	0.7929 mL	3.9646 mL	7.9293 mL
10 mM	0.3965 mL	1.9823 mL	3.9646 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4 mg/mL (15.86 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (278 KB) SDS (418 KB)

COA (255 KB) HNMR (249 KB) RP-HPLC (253 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Chenbo Zeng, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32. [Content Brief]

[2]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.9646 mL	19.8232 mL	39.6464 mL	99.1159 mL
	5 mM	0.7929 mL	3.9646 mL	7.9293 mL	19.8232 mL
	10 mM	0.3965 mL	1.9823 mL	3.9646 mL	9.9116 mL
	15 mM	0.2643 mL	1.3215 mL	2.6431 mL	6.6077 mL
	20 mM	0.1982 mL	0.9912 mL	1.9823 mL	4.9558 mL
	25 mM	0.1586 mL	0.7929 mL	1.5859 mL	3.9646 mL
	30 mM	0.1322 mL	0.6608 mL	1.3215 mL	3.3039 mL
	40 mM	0.0991 mL	0.4956 mL	0.9912 mL	2.4779 mL
	50 mM	0.0793 mL	0.3965 mL	0.7929 mL	1.9823 mL
	60 mM	0.0661 mL	0.3304 mL	0.6608 mL	1.6519 mL
	80 mM	0.0496 mL	0.2478 mL	0.4956 mL	1.2389 mL
DMSO	100 mM	0.0396 mL	0.1982 mL	0.3965 mL	0.9912 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

5-Ethynyl-2'-deoxyuridine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Ethynyl-2'-deoxyuridine61135-33-9EdUPROTAC LinkersInhibitorinhibitorinhibit

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5-Ethynyl-2'-deoxyuridine (Synonyms: EdU)

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Other Forms of 5-Ethynyl-2'-deoxyuridine:

5-Ethynyl-2'-deoxyuridine Related Antibodies

32 Publications Citing Use of MCE 5-Ethynyl-2'-deoxyuridine

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All PROTAC Linkers Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

5-Ethynyl-2'-deoxyuridine Related Antibodies

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Complete Stock Solution Preparation Table

5-Ethynyl-2'-deoxyuridine Related Classifications

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