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  4. 5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2'-deoxyuridine 

Cat. No.: HY-118411 Purity: 99.50%
COA Handling Instructions

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

5-Ethynyl-2'-deoxyuridine Chemical Structure

5-Ethynyl-2'-deoxyuridine Chemical Structure

CAS No. : 61135-33-9

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Based on 10 publication(s) in Google Scholar

5-Ethynyl-2'-deoxyuridine Related Antibodies

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Description

5-Ethynyl-2'-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation[1]. 5-Ethynyl-2'-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs[2]. 5-Ethynyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

Alkyl-Chain

 

In Vitro

5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5-Ethynyl-2'-deoxyuridine Related Antibodies
In Vivo

The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two-month old female mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Two month old female mice[1]
Dosage: 10, 20, 50, 100 or 200 mg/kg
Administration: Single injection; intraperitoneally; 4 hours after EdU injection, brains were processed for EdU staining.
Result: EdU positive cell numbers slightly increased in a dose-dependent manner both in control and running mice.
Molecular Weight

252.22

Appearance

Solid

Formula

C11H12N2O5
CAS No.
SMILES

O[C@H]1C[C@H](N2C(NC(C(C#C)=C2)=O)=O)O[C@@H]1CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (198.24 mM; Need ultrasonic)

H2O : 25 mg/mL (99.12 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9648 mL 19.8240 mL 39.6479 mL
5 mM 0.7930 mL 3.9648 mL 7.9296 mL
10 mM 0.3965 mL 1.9824 mL 3.9648 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 4 mg/mL (15.86 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.77%

COA (255 KB) HNMR (257 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References
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5-Ethynyl-2'-deoxyuridine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

5-Ethynyl-2'-deoxyuridine61135-33-9PROTAC LinkersInhibitorinhibitorinhibit

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