Cat. No.: HY-N0097

Guanosine

(Synonyms: DL-Guanosine; Vernine)

11 Cited Publications

Customer Review

Based on 11 publication(s) in Google Scholar

Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

For research use only. We do not sell to patients.

Purity: 99.23%
CAS No.: 118-00-3
Formula: C₁₀H₁₃N₅O₅
Molecular Weight:283.24

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Guanosine Chemical Structure

CAS No.: 118-00-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (11)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

IC₅₀ & Target

Human Endogenous Metabolite

All HSV Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
T-cell	IC₅₀	0.1 μM Compound: 1(a)	Compound was tested for its cytotoxicity against T- cell line Compound was tested for its cytotoxicity against T- cell line	[PMID: 3783596]
Vero	EC₅₀	0.03 μM Compound: guanosine	Antiviral activity against Vero cells, infected with HSV-1 Antiviral activity against Vero cells, infected with HSV-1	[PMID: 1720175]
Vero	IC₅₀	>100 μM Compound: guanosine	The compound was tested for toxicity in Vero cells The compound was tested for toxicity in Vero cells	[PMID: 1720175]

In Vitro

Guanosine can be phosphorylated to become guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP). These forms play important roles in various biochemical processes such as synthesis of nucleic acids and proteins, photosynthesis, muscle contraction, and intracellular signal transduction (cGMP).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 118-00-3
Appearance Solid
Molecular Weight 283.24
Formula C₁₀H₁₃N₅O₅
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)NC3=O)=C3N=C2)O1
Synonyms

DL-Guanosine; Vernine
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (11)

All Cell Imaging/StainingWBCell Proliferation/Viability Assay

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Journal Impact Factor

Mol Immunol

Antiviral activity of merimepodib against foot and mouth disease virus in vitro and in vivo. [Abstract]

2019 Oct;114:226-232. PMID: 31386979

Guanosine purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2019 Oct;114:226-232. [Abstract]

The viral VP1 protein and β-actin are detected by western blot. β-actin was used as an internal control. A serial dilution of Guanosine (from 100 μM to 25μM) are added to FMDV infected IBRS-2 cells, when treated with 25 μM merimepodib for 48 h.
Nat Commun

ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation. [Abstract]

2025 Feb 28;16(1):2071. PMID: 40021646
Nat Commun

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions. [Abstract]

2022 Oct 26;13(1):6350. PMID: 36289222
Cell Death Differ

CARM1-mediated hypoxanthine-enriched exosomes rewire inosine metabolism and impair CD8+ T cell antitumor function. [Abstract]

2026 Jan 24. PMID: 41580530
Cell Death Dis

NEK6 dampens FOXO3 nuclear translocation to stabilize C-MYC and promotes subsequent de novo purine synthesis to support ovarian cancer chemoresistance. [Abstract]

2024 Sep 10;15(9):661. PMID: 39256367

Guanosine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Sep 10;15(9):661. [Abstract]

Guanosine (60 μM; 24 h). Representative purinosome images and quantitative analysis after purine supplementation and depletion in OVCAR8 and NCI/ADR-RES cells.
Cell Rep

IMPDH2 dephosphorylation under FGFR signaling promotes S-phase progression and tumor growth. [Abstract]

2024 Dec 30;44(1):115116. PMID: 39739531

Guanosine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2024 Dec 30;44(1):115116. [Abstract]

SW620 cells with co-depletion of HPRT1 and IMPDH2 were treated with growth factors and exogenous Guanosine (25–100 μM; 72 h) as indicated. Guanosine partially restored the growth-enhancing effects of FGF2 and EGF in IMPDH2/HPRT1 co-depleted cells.
Talanta

A simple, rapid and sensitive HILIC LC-MS/MS method for simultaneous determination of 16 purine metabolites in plasma and urine. [Abstract]

2024 Jan 15:267:125171. PMID: 37696233
Molecules

In Vitro and in Vivo Antiviral Activity of Mizoribine Against Foot-And-Mouth Disease Virus. [Abstract]

2019 May 3;24(9):1723. PMID: 31058822

Guanosine purchased from MedChemExpress. Usage Cited in: Molecules. 2019 May 3;24(9):1723. [Abstract]

Guanosine supplementation attenuated the anti-FMDV effect of mizoribine. Serial dilutions of Guanosine (from 100 to 25 μM) are added to FMDV (100 TCID₅₀ O/MY98/BY/2010)-infected IBRS-2 cells, when treated with 75 μM mizoribine for 48 h. (A) The cell viability of cells and (B) FMDV VP1 levels are analyzed.
Front Cardiovasc Med

Chick early amniotic fluid component improves heart function and protects against inflammation after myocardial infarction in mice. [Abstract]

2022 Nov 16:9:1042852. PMID: 36465449
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biomed Pharmacother

Antiviral effects of selected IMPDH and DHODH inhibitors against foot and mouth disease virus. [Abstract]

2019 Oct:118:109305. PMID: 31545264

Guanosine purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Oct:118:109305. [Abstract]

FMDV-infected IBRS-2 cells were treated with AVN-944 (50 μM) or mycophenolate mofetil (50 μM) in the presence of exogenous Guanosine (50-200 μM). FMDV VP1 protein levels recovered following Guanosine supplementation.

Solvent & Solubility

In Vitro:

DMSO : ≥ 75 mg/mL (264.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (3.53 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5306 mL

17.6529 mL

35.3057 mL

5 mM

0.7061 mL

3.5306 mL

7.0612 mL

10 mM

0.3531 mL

1.7653 mL

3.5306 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 3.33 mg/mL (11.76 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. De Clercq E1. Guanosine analogues as anti-herpesvirus agents. Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1531-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.5306 mL	17.6529 mL	35.3057 mL	88.2644 mL
DMSO	5 mM	0.7061 mL	3.5306 mL	7.0611 mL	17.6529 mL
	10 mM	0.3531 mL	1.7653 mL	3.5306 mL	8.8264 mL
	15 mM	0.2354 mL	1.1769 mL	2.3537 mL	5.8843 mL
	20 mM	0.1765 mL	0.8826 mL	1.7653 mL	4.4132 mL
	25 mM	0.1412 mL	0.7061 mL	1.4122 mL	3.5306 mL
	30 mM	0.1177 mL	0.5884 mL	1.1769 mL	2.9421 mL
	40 mM	0.0883 mL	0.4413 mL	0.8826 mL	2.2066 mL
	50 mM	0.0706 mL	0.3531 mL	0.7061 mL	1.7653 mL
	60 mM	0.0588 mL	0.2942 mL	0.5884 mL	1.4711 mL
	80 mM	0.0441 mL	0.2207 mL	0.4413 mL	1.1033 mL
	100 mM	0.0353 mL	0.1765 mL	0.3531 mL	0.8826 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Guanosine

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Publications Citing Use of MedChemExpress (MCE) Guanosine

All HSV Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (11)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Guanosine Related Classifications

HY-N0097 Related Classifications