2. Anti-infection Metabolic Enzyme/Protease
  3. HSV Endogenous Metabolite
  4. Guanosine

Guanosine  (Synonyms: DL-Guanosine; Vernine)

Cat. No.: HY-N0097 Purity: 99.31%
COA Handling Instructions

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Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

For research use only. We do not sell to patients.

Guanosine Chemical Structure

Guanosine Chemical Structure

CAS No. : 118-00-3

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10 mM * 1 mL in DMSO
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Based on 8 publication(s) in Google Scholar

Other Forms of Guanosine:

Guanosine Related Antibodies

Top Publications Citing Use of Products

    Guanosine purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2019 Oct;114:226-232.  [Abstract]

    The viral VP1 protein and β-actin are detected by western blot. β-actin was used as an internal control. A serial dilution of guanosine (from 100 μM to 25μM) are added to FMDV infected IBRS-2 cells, when treated with 25 μM merimepodib for 48 h. Cell viability and VP1 protein is analysed as described above.

    Guanosine purchased from MedChemExpress. Usage Cited in: Molecules. 2019 May 3;24(9):1723.  [Abstract]

    Guanosine supplementation attenuated the anti-FMDV effect of mizoribine. Serial dilutions of guanosine (from 100 to 25 μM) are added to FMDV (100 TCID50 O/MY98/BY/2010)-infected IBRS-2 cells, when treated with 75 μM mizoribine for 48 h. (A) The cell viability of cells and (B) FMDV VP1 levels are analyzed.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Guanosine (DL-Guanosine) is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine possesses anti-HSV activity.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Guanosine can be phosphorylated to become guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP). These forms play important roles in various biochemical processes such as synthesis of nucleic acids and proteins, photosynthesis, muscle contraction, and intracellular signal transduction (cGMP).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Guanosine Related Antibodies
    Molecular Weight

    283.24

    Formula

    C10H13N5O5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=C(N)NC3=O)=C3N=C2)O1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 75 mg/mL (264.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 1 mg/mL (3.53 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5306 mL 17.6529 mL 35.3057 mL
    5 mM 0.7061 mL 3.5306 mL 7.0612 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 3.33 mg/mL (11.76 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 3.33 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.65%

    COA (251 KB) HNMR (121 KB) RP-HPLC (191 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 3.5306 mL 17.6529 mL 35.3057 mL 88.2644 mL
    DMSO 5 mM 0.7061 mL 3.5306 mL 7.0611 mL 17.6529 mL
    10 mM 0.3531 mL 1.7653 mL 3.5306 mL 8.8264 mL
    15 mM 0.2354 mL 1.1769 mL 2.3537 mL 5.8843 mL
    20 mM 0.1765 mL 0.8826 mL 1.7653 mL 4.4132 mL
    25 mM 0.1412 mL 0.7061 mL 1.4122 mL 3.5306 mL
    30 mM 0.1177 mL 0.5884 mL 1.1769 mL 2.9421 mL
    40 mM 0.0883 mL 0.4413 mL 0.8826 mL 2.2066 mL
    50 mM 0.0706 mL 0.3531 mL 0.7061 mL 1.7653 mL
    60 mM 0.0588 mL 0.2942 mL 0.5884 mL 1.4711 mL
    80 mM 0.0441 mL 0.2207 mL 0.4413 mL 1.1033 mL
    100 mM 0.0353 mL 0.1765 mL 0.3531 mL 0.8826 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Guanosine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Guanosine118-00-3DL-Guanosine VernineHSVEndogenous MetaboliteHerpes simplex virusInhibitorinhibitorinhibit

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