2'-Deoxyguanosine
Based on 6 publication(s) in Google Scholar
2’-Deoxyguanosine (Deoxyguanosine) is a purine nucleoside with a variety of biological activities. 2’-Deoxyguanosine can induce DNA division in mouse thymus cells. 2’-Deoxyguanosine is a potent cell division inhibitor in plant cells.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 961-07-9
- Formula: C10H13N5O4
- Molecular Weight:267.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2'-Deoxyguanosine
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Customer Validation & Images
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Microbiological Assay
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WB
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ELISA
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
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| C6 | IC50 |
>250 μM
Compound: 2'-Deoxyguanosine
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Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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10.1039/C3MD00159H |
| CCRF-HSB-2 | IC50 |
2.8 μg/mL
Compound: araG
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Antiproliferative effects on CCRF-HSB-2 (human leukemia) cells.
Antiproliferative effects on CCRF-HSB-2 (human leukemia) cells.
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[PMID: 9216836] |
| DU-145 | IC50 |
>250 μM
Compound: 2'-Deoxyguanosine
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Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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10.1039/C3MD00159H |
| HaCaT | IC50 |
>250 μM
Compound: 2'-Deoxyguanosine
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Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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10.1039/C3MD00159H |
| HEL | ED50 |
10.1 μg/mL
Compound: araG
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Antiviral activity against varicella zoster virus (VZV) Oka strain in HEL (human erythroleukemia) cells.
Antiviral activity against varicella zoster virus (VZV) Oka strain in HEL (human erythroleukemia) cells.
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[PMID: 9216836] |
| HEL | ED50 |
16.8 μg/mL
Compound: araG
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Antiviral activity against human cytomegalovirus (HCMV) AD 169 strain HEL (human erythroleukemia) cells.
Antiviral activity against human cytomegalovirus (HCMV) AD 169 strain HEL (human erythroleukemia) cells.
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[PMID: 9216836] |
| HEL | ED50 |
55 μg/mL
Compound: araG
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Antiviral activity against herpes simplex virus-1 VR-3 strain in HEL (human erythroleukemia) cells.
Antiviral activity against herpes simplex virus-1 VR-3 strain in HEL (human erythroleukemia) cells.
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[PMID: 9216836] |
| HEL | ED50 |
78 μg/mL
Compound: araG
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Antiviral activity against herpes simplex virus-2 (HSV-2) Ms strain in HEL (human erythroleukemia) cells.
Antiviral activity against herpes simplex virus-2 (HSV-2) Ms strain in HEL (human erythroleukemia) cells.
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[PMID: 9216836] |
| L6 | IC50 |
>250 μM
Compound: 2'-Deoxyguanosine
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Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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10.1039/C3MD00159H |
| MCF7 | IC50 |
>250 μM
Compound: 2'-Deoxyguanosine
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Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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10.1039/C3MD00159H |
| MT4 | ED50 |
158 μM
Compound: 11c
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Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells.
Dose required to inhibit cytopathic effect of human immunodeficiency virus replication in MT-4 cells.
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[PMID: 3172142] |
2'-Deoxyguanosine inhibits the clonal growth of HL-60 and K562 leukemia cells with IC50 values of 50 and 80 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 961-07-9
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Appearance Solid
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Molecular Weight 267.25
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Formula C10H13N5O4
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Color White to off-white
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SMILES
OC[C@@H](O[C@H](C1)N2C3=C(N=C2)C(NC(N)=N3)=O)[C@H]1O
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Synonyms
Deoxyguanosine; Guanine deoxyriboside
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell Rep
SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTP. [Abstract]2020 May 12;31(6):107640. PMID: 32402273 -
Cell Rep
SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation. [Abstract]2020 Mar 31;30(13):4370-4385.e7. PMID: 32234474
2'-Deoxyguanosine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Mar 31;30(13):4370-4385.e7. [Abstract]
THP-1PMA 167 cells were infected by SFTSV (MOI = 10) for 24 hours in the presence of 2'-Deoxyguanosine (dG) or 8-OH-dG. P17 in the supernatants or pro-IL-1β in the cell lysate was determined by western blot.
2'-Deoxyguanosine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2020 Mar 31;30(13):4370-4385.e7. [Abstract]
THP-1PMA 167 cells were infected by SFTSV (MOI = 10) for 24 hours in the presence of 2'-Deoxyguanosine (dG) or 8-OH-dG. IL-1β levels in the supernatants were determined by ELISA.
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Anal Chem
Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]2025 May 13;97(18):9827-9835. PMID: 40302637 -
Talanta
A simple, rapid and sensitive HILIC LC-MS/MS method for simultaneous determination of 16 purine metabolites in plasma and urine. [Abstract]2024 Jan 15:267:125171. PMID: 37696233 -
PLoS Genet
A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]2026 Jan 23;22(1):e1012013. PMID: 41575937
2'-Deoxyguanosine purchased from MedChemExpress. Usage Cited in: PLoS Genet. 2026 Jan 23;22(1):e1012013. [Abstract]
The effect of supplementing OP50-enriched metabolites to HT115 diet on the developmental rate of chca-1 mutant worms. Each metabolite was added to HT115 and seeded on NGM plates at 20 mM or their saturating concentration (final concentration: 20 mM spermidine, 6 mM thymidine, 20 mM GSSG, 20 mM 2’-deoxyuridine, 6 mM N(1)-acetylspermine, 4 mM 2’-Deoxyguanosine, 6 mM adenosine-2’,3’-cyclic monophosphate, 1 mM 2’-deoxyinosine, 20 mM spermine). Synchronized eggs were seeded on the prepared plates to record developmental rate.
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Solvent & Solubility
DMSO : 100 mg/mL (374.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.35 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ross DD, et al. Effects of deoxynucleosides on cultured human leukemia cell growth and deoxynucleotide pools. Cancer Res. 1981 Nov;41(11 Pt 1):4493-8. [Content Brief]
[2]. Kizaki H, et al. Adenosine, deoxyadenosine, and deoxyguanosine induce DNA cleavage in mouse thymocytes. J Immunol. 1988 Sep 1;141(5):1652-7. [Content Brief]
[3]. Brulfert A, et al. Deoxyguanosine, a potent cytokinesis inhibitor in plant cells. Experientia. 1974 Sep 15;30(9):1010-1. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.7418 mL | 18.7091 mL | 37.4181 mL | 93.5454 mL |
| 5 mM | 0.7484 mL | 3.7418 mL | 7.4836 mL | 18.7091 mL | |
| 10 mM | 0.3742 mL | 1.8709 mL | 3.7418 mL | 9.3545 mL | |
| 15 mM | 0.2495 mL | 1.2473 mL | 2.4945 mL | 6.2364 mL | |
| 20 mM | 0.1871 mL | 0.9355 mL | 1.8709 mL | 4.6773 mL | |
| 25 mM | 0.1497 mL | 0.7484 mL | 1.4967 mL | 3.7418 mL | |
| 30 mM | 0.1247 mL | 0.6236 mL | 1.2473 mL | 3.1182 mL | |
| 40 mM | 0.0935 mL | 0.4677 mL | 0.9355 mL | 2.3386 mL | |
| 50 mM | 0.0748 mL | 0.3742 mL | 0.7484 mL | 1.8709 mL | |
| 60 mM | 0.0624 mL | 0.3118 mL | 0.6236 mL | 1.5591 mL | |
| 80 mM | 0.0468 mL | 0.2339 mL | 0.4677 mL | 1.1693 mL | |
| 100 mM | 0.0374 mL | 0.1871 mL | 0.3742 mL | 0.9355 mL |
2'-Deoxyguanosine Related Classifications
HY-17563 Related Classifications
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