2'-Deoxyadenosine monohydrate
Based on 1 Customer Validation
2′-Deoxyadenosine monohydrate is an adenine nucleoside that inhibits glucose-stimulated insulin release. 2′-Deoxyadenosine monohydrate inhibits glucose-stimulated increases seen in islet cyclic AMP (cAMP) accumulation. 2'-Deoxyadenosine monohydrate activates caspase-3 and promotes apoptosis. 2'-Deoxyadenosine monohydrate inhibits the activity of S-adenosyl-L-homocysteine hydrolase (SAHH). 2'-Deoxyadenosine monohydrate inhibits the growth of various cells. 2'-Deoxyadenosine monohydrate has an anticancer effect on colon cancer.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 16373-93-6
- Formula: C10H15N5O4
- Molecular Weight:269.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Microbial Metabolite |
Human Endogenous Metabolite |
2′-Deoxyadenosine (10 mM) monohydrate does not inhibit insulin biosynthesis or [U-14C]glucose oxidation at concentrations of the nucleosides that gave maximal inhibition of insulin release. 2'-Deoxyadenosine monohydrate produces marked inhibition of the glucose-stimulated increases seen in islet cyclic AMP accumulation[1].
2'-Deoxyadenosine (0.01-0.5 mM; 24-72 h) monohydrate inhibits the growth of LoVo human colon carcinoma cells in a concentration- and time-dependent manner and promotes apoptosis, as indicated by characteristic apoptotic morphology[2].
2'-Deoxyadenosine monohydrate inhibits S-adenosyl-L-homocysteine hydrolase activity in crude extracts of L1210 leukemia cells in a time-dependent manner when assays are conducted in the presence of Deoxycoformycin (HY-A0006)[3].
2'-Deoxyadenosine (0.1-2 mM; up to 3 h) monohydrate inhibits pig keratinocyte outgrowth, [3H] thymidine uptake, and mitosis[4].
2'-Deoxyadenosine (0.1 mM; 4 days) monohydrate in combination with 1 μM dCF inhibits the growth of LoVo cells in a cell-density-dependent manner[5].
2'-Deoxyadenosine (100 μM; 3 days) monohydrate in the presence of 3 μM Deoxycoformycin (HY-A0006) is toxic to rat chromaffin cells, causing apoptosis-like cell death, as shown by morphological changes, DNA fragmentation, and increased dATP levels[6].
2'-Deoxyadenosine (0.1 mM) monohydrate in the presence of 0.01 mM dCF in vitro leads to a significant increase in dATP levels in mouse embryos[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LoVo human colon carcinoma cell line
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Concentration:0.01 mM, 0.02 mM, 0.05 mM, 0.1 mM, 0.2 mM, 0.5 mM24 h, 48 h, 72 h
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Incubation Time:24 h, 48 h, 72 h
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Result:Increased the percentage of apoptotic cells, reaching a maximum of approximately 25% after 72 h (0.5 mM).
Processed procaspase-3 and released cytochrome c.
Chemical Information
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CAS No. 16373-93-6
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Appearance Solid
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Molecular Weight 269.26
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Formula C10H15N5O4
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Color White to off-white
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SMILES
O[C@H]1C[C@H](N2C(N=CN=C3N)=C3N=C2)O[C@@H]1CO.O
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Structure Classification
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Initial Source
Streptomyces jiujiangensis
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (185.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 7.14 mg/mL (26.52 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Iain L. Campbell, et al. Effects of adenosine, 2-deoxyadenosine and N6-phenylisopropyladenosine on rat islet function and metabolism. Biochem J (1982) 204 (3): 689-696. [Content Brief]
[2]. Giannecchini M, et al. 2'-Deoxyadenosine causes apoptotic cell death in a human colon carcinoma cell line. J Biochem Mol Toxicol. 2003;17(6):329-37. [Content Brief]
[3]. Cass CE, et al. Effects of 2'-deoxyadenosine, 9-beta-D-arabinofuranosyladenine, and related compounds on S-adenosyl-L-homocysteine hydrolase activity in synchronous and asynchronous cultured cells. Cancer Res. 1982 Dec;42(12):4991-8. [Content Brief]
[4]. Iizuka H, et al. Effects of adenosine and 2'-deoxyadenosine on epidermal keratinocyte proliferation: its relation to cyclic AMP formation. J Invest Dermatol. 1984 Jun;82(6):608-12. [Content Brief]
[5]. Bemi V, et al. Deoxyadenosine metabolism in a human colon-carcinoma cell line (LoVo) in relation to its cytotoxic effect in combination with deoxycoformycin. Int J Cancer. 1998 Mar 2;75(5):713-20. [Content Brief]
[6]. Wakade AR, et al. 2'-deoxyadenosine induces apoptosis in rat chromaffin cells. J Neurochem. 1996 Dec;67(6):2273-81. [Content Brief]
[7]. Gao X, et al. Activation of apoptosis in early mouse embryos by 2'-deoxyadenosine exposure. Teratology. 1994 Jan;49(1):1-12. [Content Brief]
[8]. Kuttesch JF Jr, et al. Renal handling of 2'-deoxyadenosine and adenosine in humans and mice. Cancer Chemother Pharmacol. 1982;8(2):221-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.7139 mL | 18.5694 mL | 37.1388 mL | 92.8471 mL |
| 5 mM | 0.7428 mL | 3.7139 mL | 7.4278 mL | 18.5694 mL | |
| 10 mM | 0.3714 mL | 1.8569 mL | 3.7139 mL | 9.2847 mL | |
| 15 mM | 0.2476 mL | 1.2380 mL | 2.4759 mL | 6.1898 mL | |
| 20 mM | 0.1857 mL | 0.9285 mL | 1.8569 mL | 4.6424 mL | |
| 25 mM | 0.1486 mL | 0.7428 mL | 1.4856 mL | 3.7139 mL | |
| DMSO | 30 mM | 0.1238 mL | 0.6190 mL | 1.2380 mL | 3.0949 mL |
| 40 mM | 0.0928 mL | 0.4642 mL | 0.9285 mL | 2.3212 mL | |
| 50 mM | 0.0743 mL | 0.3714 mL | 0.7428 mL | 1.8569 mL | |
| 60 mM | 0.0619 mL | 0.3095 mL | 0.6190 mL | 1.5475 mL | |
| 80 mM | 0.0464 mL | 0.2321 mL | 0.4642 mL | 1.1606 mL | |
| 100 mM | 0.0371 mL | 0.1857 mL | 0.3714 mL | 0.9285 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2'-Deoxyadenosine monohydrate Related Classifications
HY-W011683 Related Classifications
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