Cat. No.: HY-A0006

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Pentostatin

(Synonyms: CI-825; Deoxycoformycin)

10 Cited Publications

Customer Review

Based on 10 publication(s) in Google Scholar

Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with K_i of 2.5 pM.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 53910-25-1
Formula: C₁₁H₁₆N₄O₄
Molecular Weight:268.27

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Pentostatin Chemical Structure

CAS No.: 53910-25-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
- Clinical Trial
Chemical Information
Publications (10)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with K_i of 2.5 pM.

IC₅₀ & Target

Ki: 2.5 pM (adenosine deaminase)

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	IC₅₀	0.25 μM Compound: pentostatin	Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin	[PMID: 3489838]
L1210	IC₅₀	1.9 μM Compound: pentostatin	Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin	[PMID: 3489838]

In Vivo

In ECP and Pentostatin (CI-825; Deoxycoformycin; 4 mg/m², i.v.) treatment, all dogs develop granulocytopenia with <500 granulocytes/μL from day 4. Thrombocytopenia (<20,000 platelets/μL) occurs from day 7 after HCT with nadirs of 3000 to 14000 platelets/μL^[1]. Pentostatin (2 mg/kg) in combination with cordycepin (2 mg/kg) is 100% effective in the T. evansi-infected mice. There is an increase in levels of some biochemical parameters, especially on liver enzymes, which are accompanied by histological lesions in the liver and kidneys. Pentostatin individually has no curative effect on infected groups^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 53910-25-1
Appearance Solid
Molecular Weight 268.27
Formula C₁₁H₁₆N₄O₄
Color White to off-white
SMILES

O[C@H]1C(N=CN2[C@H](O[C@@H]3CO)C[C@@H]3O)=C2NC=NC1
Synonyms

CI-825; Deoxycoformycin
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (10)

All In Vivo Efficacy StudyCell Proliferation/Viability AssayWB

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Journal Impact Factor

Sci Transl Med

Chemical perturbations reveal that RUVBL2 regulates the circadian phase in mammals. [Abstract]

2020 May 6;12(542):eaba0769. PMID: 32376767
Adv Sci (Weinh)

Targeting Adenosine A2b Receptor Promotes Penile Rehabilitation of Refractory Erectile Dysfunction. [Abstract]

2024 Jun 14:e2306514. PMID: 38874549

Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514. [Abstract]

Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated ICPs of C57BL/6 mice.

Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514. [Abstract]

Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated AUCs of C57BL/6 mice.
J Nanobiotechnology

Role of A2B adenosine receptor-dependent adenosine signaling in multi-walled carbon nanotube-triggered lung fibrosis in mice. [Abstract]

2019 Mar 29;17(1):45. PMID: 30922349
Virulence

Streptococcus suis synthesizes deoxyadenosine and adenosine by 5'-nucleotidase to dampen host immune responses. [Abstract]

2018;9(1):1509-1520. PMID: 30221577

Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]

The viability of RAW 264.7 cells treated with or without Pentostatin (Deoxycoformycin; dCF) (100 μM).

Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]

Pentostatin (Deoxycoformycin; dCF) (100 μM; 12-24 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with dAdo.

Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]

Pentostatin (Deoxycoformycin; dCF) (100 μM; 12 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with tNT+dAMP.
PLoS Pathog

Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus-induced lung injury. [Abstract]

2020 Mar 16;16(3):e1008341. PMID: 32176725
J Neurochem

Metabolomics-driven identification of adenosine deaminase as therapeutic target in a mouse model of Parkinson's disease. [Abstract]

2019 Aug;150(3):282-295. PMID: 31121068
Neurobiol Stress

Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1. [Abstract]

2021 Sep 11;15:100396. PMID: 34568523
J Immunol

Adenosine Alleviates Necrotizing Enterocolitis by Enhancing the Immunosuppressive Function of Myeloid-Derived Suppressor Cells in Newborns. [Abstract]

2022 Jul 15;209(2):401-411. PMID: 35777852
Authorea

2025 Oct 7.
bioRxiv

Multiplexed single-cell lineage tracing of mitotic kinesin inhibitor resistance in glioblastoma. [Abstract]

2023 Sep 12:2023.09.09.557001. PMID: 37745469

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (372.75 mM; Need ultrasonic)

DMSO : ≥ 50 mg/mL (186.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7275 mL

18.6377 mL

37.2755 mL

5 mM

0.7455 mL

3.7275 mL

7.4551 mL

10 mM

0.3728 mL

1.8638 mL

3.7275 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline

Solubility: 100 mg/mL (372.75 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protocol

Animal Administration^[1]

All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linear accelerator. Dogs in group A1receive ECP administered on days −2 and −1 with TBI on day 0 and dogs in group A2receive ECP on days −6 and −5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m² on days −4 and −3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV into recipients at a median dose of 2.9 (range, 1.9 to 6.1) ×10⁸ total nucleated cells (TNC)/kg. The day of marrow grafting is designated as day 0. In addition to marrow graft, recipients are given IV infusions of peripheral blood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median dose of 2.3 (range, 1.2 to 6.9) ×10⁸ TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of 0.4 mg/kg intravenously is used as postgrafting immunosuppression and administered on days +1, +3, +6 and +11, then weekly thereafter until day 102.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bethge WA, et al. Extracorporeal photopheresis combined with pentostatin in the conditioning regimen for canine hematopoietic cell transplantation does not prevent GVHD. Bone Marrow Transplant. 2014 Sep;49(9):1198-204. [Content Brief]

[2]. Dalla Rosa L, et al. Cordycepin (3'-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi. Parasitology. 2013 Apr;140(5):663-71. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	3.7275 mL	18.6377 mL	37.2755 mL	93.1887 mL
	5 mM	0.7455 mL	3.7275 mL	7.4551 mL	18.6377 mL
	10 mM	0.3728 mL	1.8638 mL	3.7275 mL	9.3189 mL
	15 mM	0.2485 mL	1.2425 mL	2.4850 mL	6.2126 mL
	20 mM	0.1864 mL	0.9319 mL	1.8638 mL	4.6594 mL
	25 mM	0.1491 mL	0.7455 mL	1.4910 mL	3.7275 mL
	30 mM	0.1243 mL	0.6213 mL	1.2425 mL	3.1063 mL
	40 mM	0.0932 mL	0.4659 mL	0.9319 mL	2.3297 mL
	50 mM	0.0746 mL	0.3728 mL	0.7455 mL	1.8638 mL
	60 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5531 mL
	80 mM	0.0466 mL	0.2330 mL	0.4659 mL	1.1649 mL
	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pentostatin

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Publications Citing Use of MedChemExpress (MCE) Pentostatin

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (10)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pentostatin Related Classifications

Cat. No.: HY-A0006