1. Metabolic Enzyme/Protease
  2. Adenosine Deaminase
  3. Pentostatin

Pentostatin (Synonyms: CI-825; Deoxycoformycin)

Cat. No.: HY-A0006 Purity: 99.93%
Handling Instructions

Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with Ki of 2.5 pM.

For research use only. We do not sell to patients.

Pentostatin Chemical Structure

Pentostatin Chemical Structure

CAS No. : 53910-25-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 120 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 120 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 109 In-stock
Estimated Time of Arrival: December 31
50 mg USD 493 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Pentostatin purchased from MCE. Usage Cited in: Virulence. 2018;9(1):1509-1520.

    Western blot analysis shows that the band of active caspase-3 is detected in RAW 264.7 cells treated with 55 μM dAdo and 100 μM dCF for 12 h, 16 h, or 24 h.
    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with Ki of 2.5 pM.

    IC50 & Target

    Ki: 2.5 pM (adenosine deaminase)

    In Vivo

    In ECP and Pentostatin (CI-825; Deoxycoformycin; 4 mg/m2, i.v.) treatment, all dogs develop granulocytopenia with <500 granulocytes/μL from day 4. Thrombocytopenia (<20,000 platelets/μL) occurs from day 7 after HCT with nadirs of 3000 to 14000 platelets/μL[1]. Pentostatin (2 mg/kg) in combination with cordycepin (2 mg/kg) is 100% effective in the T. evansi-infected mice. There is an increase in levels of some biochemical parameters, especially on liver enzymes, which are accompanied by histological lesions in the liver and kidneys. Pentostatin individually has no curative effect on infected groups[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    268.27

    Formula

    C₁₁H₁₆N₄O₄

    CAS No.
    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (372.76 mM; Need ultrasonic)

    DMSO : ≥ 50 mg/mL (186.38 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7276 mL 18.6379 mL 37.2759 mL
    5 mM 0.7455 mL 3.7276 mL 7.4552 mL
    10 mM 0.3728 mL 1.8638 mL 3.7276 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [1]

    All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linear accelerator. Dogs in group A1receive ECP administered on days −2 and −1 with TBI on day 0 and dogs in group A2receive ECP on days −6 and −5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m2 on days −4 and −3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV into recipients at a median dose of 2.9 (range, 1.9 to 6.1) ×108 total nucleated cells (TNC)/kg. The day of marrow grafting is designated as day 0. In addition to marrow graft, recipients are given IV infusions of peripheral blood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median dose of 2.3 (range, 1.2 to 6.9) ×108 TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of 0.4 mg/kg intravenously is used as postgrafting immunosuppression and administered on days +1, +3, +6 and +11, then weekly thereafter until day 102.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.93%

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    Keywords:

    PentostatinCI-825 DeoxycoformycinCI825CI 825Adenosine DeaminaseInhibitorinhibitorinhibit

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    Cat. No.:
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