6-(Dimethylamino)purine
Based on 1 Customer Validation
6-(Dimethylamino) purine (6-Dimethylaminopurine) is a serine threonine protein kinase inhibitor. 6-(Dimethylamino) purine can inhibit prolactin induced expression of lactoprotein genes in rabbit mammary gland cells. 6-(Dimethylamino) purine can affect the maturation of mammalian oocytes. 6-(Dimethylamino) purine can lead to downregulation of genes related to cell proliferation and cell cycle progression, such as proliferating cell nuclear antigen, insulin-like gene 1, and serine protease inhibitor 2 genes, and induce apoptosis in lymphoma cells (apoptosis).
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 938-55-6
- Formula: C7H9N5
- Molecular Weight:163.18
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
protein kinase[1].
6-(Dimethylamino) purine (2.5 mM. 3 h) accelerates the transition to interphase in activated mouse oocytes by inhibiting protein kinases[1]. 6-(Dimethylamino) purine blocks DNA synthesis in activated mammalian oocytes[2]. 6-(Dimethylamino) purine inhibits prolactin induced expression of milk protein genes in rabbit mammary gland cells[3]. 6-(Dimethylamino) purine (0.5 mM, 6 h) affects sperm motility and vitality in mammals and insects by regulating protein phosphorylation non phosphorylation changes[4]. 6-(Dimethylamino) purine (5 mM, 16 h) induces apoptosis in lymphoma cells by inhibiting protein kinases, leading to a significant downregulation of all genes related to cell proliferation and cell cycle progression, such as proliferating cell nuclear antigen, insulin-derived gene 1, and serine protease inhibitor 2 genes[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Oocytes
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Concentration:2.5 mM
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Incubation Time:3 h
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Result:Induced protein dephosphorylation.
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Cell Line:The fly and mouse sperm
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Concentration:0.5 mM
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Incubation Time:6 h
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Result:Reduced the production of dynein and β - actin in fly sperm, while increasing the levels of both proteins in mice.
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Cell Line:Human lymphoma U937 cell
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Concentration:5 mM
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Incubation Time:16 h
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Result:Induced apparent DNA fragmentation, phosphatidylserine externalization and lowering of the mitochondrial membrane potential and cell apoptosis.
Chemical Information
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CAS No. 938-55-6
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Appearance Solid
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Molecular Weight 163.18
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Formula C7H9N5
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Color Off-white to light yellow
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SMILES
CN(C)C1=C2NC=NC2=NC=N1
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Synonyms
6-Dimethylaminopurine; 6-DMAP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 33.33 mg/mL (204.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (12.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (12.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Szöllösi MS, et al. Inhibition of protein kinases by 6-dimethylaminopurine accelerates the transition to interphase in activated mouse oocytes. J Cell Sci. 1993 Mar;104 ( Pt 3):861-72. [Content Brief]
[2]. Ledda S, et al. The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes. Zygote. 1996 Feb;4(1):7-9. [Content Brief]
[3]. Bayat-Sarmadi M, et al. 6-Dimethyl amino purine and 2-amino purine inhibit the induction of expression of milk protein genes by prolactin. Biosci Rep. 1992 Jun;12(3):189-97. [Content Brief]
[4]. Ogneva IV. Mouse and Fly Sperm Motility Changes Differently under Modelling Microgravity. Curr Issues Mol Biol. 2021 Jul 5;43(2):590-604. [Content Brief]
[5]. Nakashima M, et al. Apoptosis induced by an alkylated purine, 6-dimethylaminopurine, and changes in gene expression in human lymphoma U937 cells. Anticancer Res. 2008 Mar-Apr;28(2A):609-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.1282 mL | 30.6410 mL | 61.2820 mL | 153.2051 mL |
| 5 mM | 1.2256 mL | 6.1282 mL | 12.2564 mL | 30.6410 mL | |
| 10 mM | 0.6128 mL | 3.0641 mL | 6.1282 mL | 15.3205 mL | |
| 15 mM | 0.4085 mL | 2.0427 mL | 4.0855 mL | 10.2137 mL | |
| 20 mM | 0.3064 mL | 1.5321 mL | 3.0641 mL | 7.6603 mL | |
| 25 mM | 0.2451 mL | 1.2256 mL | 2.4513 mL | 6.1282 mL | |
| 30 mM | 0.2043 mL | 1.0214 mL | 2.0427 mL | 5.1068 mL | |
| 40 mM | 0.1532 mL | 0.7660 mL | 1.5321 mL | 3.8301 mL | |
| 50 mM | 0.1226 mL | 0.6128 mL | 1.2256 mL | 3.0641 mL | |
| 60 mM | 0.1021 mL | 0.5107 mL | 1.0214 mL | 2.5534 mL | |
| 80 mM | 0.0766 mL | 0.3830 mL | 0.7660 mL | 1.9151 mL | |
| 100 mM | 0.0613 mL | 0.3064 mL | 0.6128 mL | 1.5321 mL |
6-(Dimethylamino)purine Related Classifications
HY-W010128 Related Classifications
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