2. GPCR/G Protein Metabolic Enzyme/Protease
  3. G protein-coupled Bile Acid Receptor 1 Endogenous Metabolite
  4. Hyodeoxycholic acid

Hyodeoxycholic acid  (Synonyms: HDCA; ヒオデオキシコール酸)

製品番号: HY-N0169 純度: 99.94%
COA 取扱説明書 Technical Support

Hyodeoxycholic acid is a secondary bile acid formed in the small intestine by the gut flora, and acts as a TGR5 (GPCR19) agonist, with an EC50 of 31.6 µM in CHO cells.

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CAS 番号 : 83-49-8

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
100 mg $25 在庫あり
500 mg $45 在庫あり
1 g $60 在庫あり
5 g $96 在庫あり
10 g $125 在庫あり
25 g $200 在庫あり
50 g $300 在庫あり
> 100 g   お問い合わせ  

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カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of Hyodeoxycholic acid:

Hyodeoxycholic acid 関連抗体

Top Publications Citing Use of Products

    Hyodeoxycholic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Jul 11:S2090-1232(25)00543-0.  [Abstract]

    Representative immunoblots treated with Hyodeoxycholic acid (HDCA) (20 mg/kg, i.g.).

    Hyodeoxycholic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Jul 11:S2090-1232(25)00543-0.  [Abstract]

    PWT and PWL of mice following Hyodeoxycholic acid (HDCA) (20 mg/kg, i.g.) treatment, relative expression levels of FXR mRNA.

    Hyodeoxycholic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Jul 11:S2090-1232(25)00543-0.  [Abstract]

    Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum treated with Hyodeoxycholic acid (HDCA) (20 mg/kg, i.g.).

    Hyodeoxycholic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Jul 11:S2090-1232(25)00543-0.  [Abstract]

    Immunofluorescence staining and mean fluorescence intensity of Mucin-2 treated with Hyodeoxycholic acid (HDCA) (20 mg/kg, i.g.).

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Human Endogenous Metabolite Microbial Metabolite

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Hyodeoxycholic acid is a secondary bile acid formed in the small intestine by the gut flora, and acts as a TGR5 (GPCR19) agonist, with an EC50 of 31.6 µM in CHO cells.

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO EC50
    31.6 μM
    Compound: 10, HDCA
    Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
    Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
    		[PMID: 18307294]
    HEK-293T EC50
    >150 μM
    Compound: HDCA
    Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
    Agonist activity at VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
    		[PMID: 26774929]
    HEK-293T IC50
    >50 μM
    Compound: HDCA
    Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
    Antagonist activity against VP16 tagged-VDR-LBD (unknown origin) expressed in HEK293T cells assessed as inhibition of 1,25-dihydroxyvitamin D3-induced SRC1 coactivator peptide recruitment after 16 hrs by luciferase reporter gene based two hybrid assay
    		[PMID: 26774929]
    HT-29 IC50
    >80 μM
    Compound: 1; HDCA
    Antiproliferative activity against human HT-29 cells after 48 hrs by SRB assay
    Antiproliferative activity against human HT-29 cells after 48 hrs by SRB assay
    		[PMID: 27448915]
    PC-3M IC50
    >80 μM
    Compound: 1; HDCA
    Antiproliferative activity against human PC3M cells after 48 hrs by SRB assay
    Antiproliferative activity against human PC3M cells after 48 hrs by SRB assay
    		[PMID: 27448915]
    体外実験

    Hyodeoxycholic acid is a secondary hydrophilic bile acid formed in the small intestine by the gut flora, and acts as an agonist of TGR5, with an EC50 of 31.6 μM in CHO cells[1]. Hyodeoxycholic acid (50, 100 μM) increases the expression of genes (Abca1, Abcg1, and Apoe) involved in cholesterol efflux in RAW 264.7 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hyodeoxycholic acid 関連抗体
    体内実験

    Hyodeoxycholic acid (HDCA; 1.25% (wt/wt)) obviously decreases fat mass and increases lean mass but does not raise the serum levels of any organ toxicity markers in LDLRKO mice. Hyodeoxycholic acid inhibits atherosclerotic lesion formation in LDLRKO at multiple sites, improves plasma lipoprotein profiles, decreases plasma glucose level and intestinal cholesterol absorption efficiency and increases daily cholesterol excretion through fecal output. Hyodeoxycholic acid also improves HDL function as measured by a cholesterol efflux assay[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    392.57

    分子式

    C24H40O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])C[C@H](O)[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (254.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5473 mL 12.7366 mL 25.4732 mL
    5 mM 0.5095 mL 2.5473 mL 5.0946 mL
    10 mM 0.2547 mL 1.2737 mL 2.5473 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.37 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.94%

    COA (250 KB) RP-HPLC (243 KB)

    取扱説明書 (2659 KB)
    参考文献
    動物実験
    [2]

    Mice[2]
    For atherosclerosis studies, 8-wk-old female LDLRKO mice are fed a Western diet (21% fat, 0.15% cholesterol; TD.88137) for 8 wk. One group of mice (baseline group) is euthanized at this time point for lesion measurement in the aortic root region and in the innominate artery. Atherosclerotic lesion in the whole aorta is not examined in the baseline group. The remaining mice are then divided into 2 groups and fed the following diets for another 15 wk before euthanasia: group 1, chow diet (5% fat, AIN-76A Rodent Diet); and group 2, chow diet + 1.25% (wt/wt) Hyodeoxycholic acid. For other studies, 8-wk-old female LDLRKO mice are fed a chow diet or chow diet + 1.25% Hyodeoxycholic acid for 3 wk before phenotype measurements. Food consumption and body weight are recorded weekly. Animals are measured for total body fat mass and lean mass by magnetic resonance imaging (MRI) using Bruker Minispec with software from Eco Medical Systems[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5473 mL 12.7366 mL 25.4732 mL 63.6829 mL
    5 mM 0.5095 mL 2.5473 mL 5.0946 mL 12.7366 mL
    10 mM 0.2547 mL 1.2737 mL 2.5473 mL 6.3683 mL
    15 mM 0.1698 mL 0.8491 mL 1.6982 mL 4.2455 mL
    20 mM 0.1274 mL 0.6368 mL 1.2737 mL 3.1841 mL
    25 mM 0.1019 mL 0.5095 mL 1.0189 mL 2.5473 mL
    30 mM 0.0849 mL 0.4246 mL 0.8491 mL 2.1228 mL
    40 mM 0.0637 mL 0.3184 mL 0.6368 mL 1.5921 mL
    50 mM 0.0509 mL 0.2547 mL 0.5095 mL 1.2737 mL
    60 mM 0.0425 mL 0.2123 mL 0.4246 mL 1.0614 mL
    80 mM 0.0318 mL 0.1592 mL 0.3184 mL 0.7960 mL
    100 mM 0.0255 mL 0.1274 mL 0.2547 mL 0.6368 mL
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    Hyodeoxycholic acid Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hyodeoxycholic acid83-49-8HDCAG protein-coupled Bile Acid Receptor 1Endogenous MetaboliteG-protein coupled receptor 19GPCR19TGR5GPBAR1Inhibitorinhibitorinhibit

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    製品名:
    Hyodeoxycholic acid
    製品番号:
    HY-N0169
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