2. Anti-infection Apoptosis Autophagy
  3. Bacterial Antibiotic Apoptosis Autophagy
  4. Nitroxoline

Nitroxoline  (Synonyms: 8-Hydroxy-5-nitroquinoline; 5-Nitro-8-quinolinol; 8-ヒドロキシ-5-ニトロキノリン)

製品番号: HY-B1159 純度: 99.90%
COA 取扱説明書 Technical Support

Nitroxoline (8-Hydroxy-5-nitroquinoline), an antibiotic, is an orally active antibiofilm agent. Nitroxoline reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc. Nitroxoline can be used for the urinary tract infections and cancer research.

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Nitroxoline

Nitroxoline 構造式

CAS 番号 : 4008-48-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
500 mg $25 在庫あり
1 g $40 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of Nitroxoline:

Nitroxoline 関連抗体

Top Publications Citing Use of Products

Antibiotic アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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  • 純度とドキュメンテーション

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製品説明

Nitroxoline (8-Hydroxy-5-nitroquinoline), an antibiotic, is an orally active antibiofilm agent. Nitroxoline reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc. Nitroxoline can be used for the urinary tract infections and cancer research[1][2][3].

Cellular Effect
Cell Line Type Value Description References
HUVEC IC50
1.9 3
Compound: 10; APL-1202
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
[PMID: 30939017]
BTI-TN-5B1-4 IC50
55 1
Compound: 10; APL-1202
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
[PMID: 30939017]
BTI-TN-5B1-4 IC50
55 1
Compound: 10; APL-1202
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
[PMID: 30939017]
HUVEC IC50
283 6
Compound: 4a
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
[PMID: 34865870]
HUVEC IC50
1.9 3
Compound: 10; APL-1202
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
[PMID: 30939017]
HUVEC IC50
1.9 3
Compound: 10; APL-1202
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against HUVEC measured after 24 hrs by [3H]-thymidine incorporation assay
[PMID: 30939017]
BTI-TN-5B1-4 IC50
55 1
Compound: 10; APL-1202
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
Inhibition of recombinant human N-terminal His-tagged MetAP2 expressed in baculovirus infected BTI-TN-5B1-4 insect cells using Met-Pro-pNA as substrate preincubated for 20 mins followed by substrate addition and measured after 30 mins
[PMID: 30939017]
HUVEC IC50
283 6
Compound: 4a
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
[PMID: 34865870]
MV4-11 IC50
0.98 3
Compound: 11
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
[PMID: 30529546]
MV4-11 IC50
4.9 3
Compound: 11
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
[PMID: 30529546]
HUVEC IC50
283 6
Compound: 4a
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
Cytotoxicity against human HUVEC cells assessed as metabolic activity measured after 24 hrs by cell-titer blue assay
[PMID: 34865870]
MV4-11 IC50
4.9 3
Compound: 11
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
[PMID: 30529546]
MV4-11 IC50
0.98 3
Compound: 11
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
[PMID: 30529546]
MV4-11 IC50
0.98 3
Compound: 11
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
Inhibition of BRD4 in human MV4-11 cells using biotin labelled N-C:SGRG-K(Ac)-GG-K(Ac)-GLG-K(Ac)-GGA-K(Ac)-RHRKVGG-K as substrate preincubated for 15 mins followed by substrate addition measured after 5 mins by alpha screen assay
[PMID: 30529546]
MV4-11 IC50
4.9 3
Compound: 11
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter Glo luminescent assay
[PMID: 30529546]
体外実験

Biofilm mass synthesis is reduced by up to 80% at sub-MIC Nitroxoline (0.25-16 μg/mL) concentrations in Pseudomonas aeruginosa, and structures formed are reticulate rather than compact. In preformed biofilms, viable cell counts are reduced by 4 logs at therapeutic concentrations[1].
Nitroxoline (2.5-20 μM; 24 hours) effectively inhibits cell survival of small-cell lung cancer (SCLC) cells, and induces SCLC cell apoptosis by suppressing antiapoptotic proteins (such as Bcl-2 and MCL1) and upregulating proapoptotic protein Bim. Nitroxoline is found to downregulate MDM2 expression by inducing its proteasomal degradation, and thus upregulates p53 expression, which is a substrate protein of MDM2[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nitroxoline 関連抗体

Cell Viability Assay[2]

Cell Line: H446, H1882, H1417, and H1688 cells
Concentration: 2.5 μM, 5 μM, 10 μM, 20 μM
Incubation Time: 24 hours
Result: Effectively inhibit cell survival of small-cell lung cancer (SCLC) cells.

Western Blot Analysis[2]

Cell Line: H446, H1882, H1417 cells
Concentration: 5 μM, 10 μM, 20 μM
Incubation Time: 24 hours
Result: Suppressed antiapoptotic proteins (such as Bcl-2 and MCL1) and upregulated proapoptotic protein Bim.
体内実験

Nitroxoline (15-60mg/kg; orally gavage) results in significant inhibition of tumor growth in the C3H/He mice bladder cancer subcutaneous model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C3H/He mice bladder injected with MBT-2 cells[3]
Dosage: 15 mg/kg or 60mg/kg
Administration: Orally gavage; five times a week on days 1, 2, 4, 5, 7, 8, 10, and 11.
Result: Resulted in significant inhibition of tumor growth.
分子量

190.16

分子式

C9H6N2O3
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=C2N=CC=CC2=C([N+]([O-])=O)C=C1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (262.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.2588 mL 26.2939 mL 52.5878 mL
5 mM 1.0518 mL 5.2588 mL 10.5176 mL
10 mM 0.5259 mL 2.6294 mL 5.2588 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 99.90%

COA (253 KB) LCMS (92 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2588 mL 26.2939 mL 52.5878 mL 131.4696 mL
5 mM 1.0518 mL 5.2588 mL 10.5176 mL 26.2939 mL
10 mM 0.5259 mL 2.6294 mL 5.2588 mL 13.1470 mL
15 mM 0.3506 mL 1.7529 mL 3.5059 mL 8.7646 mL
20 mM 0.2629 mL 1.3147 mL 2.6294 mL 6.5735 mL
25 mM 0.2104 mL 1.0518 mL 2.1035 mL 5.2588 mL
30 mM 0.1753 mL 0.8765 mL 1.7529 mL 4.3823 mL
40 mM 0.1315 mL 0.6573 mL 1.3147 mL 3.2867 mL
50 mM 0.1052 mL 0.5259 mL 1.0518 mL 2.6294 mL
60 mM 0.0876 mL 0.4382 mL 0.8765 mL 2.1912 mL
80 mM 0.0657 mL 0.3287 mL 0.6573 mL 1.6434 mL
100 mM 0.0526 mL 0.2629 mL 0.5259 mL 1.3147 mL
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Nitroxoline Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitroxoline4008-48-48-Hydroxy-5-nitroquinoline 5-Nitro-8-quinolinolBacterialAntibioticApoptosisAutophagyInhibitorinhibitorinhibit

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