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Phenytoin (sodium)  (Synonyms: 5,5-Diphenylhydantoin (sodium salt))

製品番号: HY-B0448A 純度: 99.78%
COA 取扱説明書 Technical Support

Phenytoin sodium (5,5-Diphenylhydantoin sodium salt) is a potent Voltage-gated Na+ channels (VGSCs) blocker. Phenytoin has antiepileptic activity and reduces breast tumour growth and metastasis in mice.

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CAS 番号 : 630-93-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
500 mg $55 在庫あり
1 g $66 在庫あり
5 g $106 在庫あり
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カスタマーレビュー

Based on 6 publication(s) in Google Scholar

Other Forms of Phenytoin sodium:

Phenytoin (sodium) 関連抗体

Top Publications Citing Use of Products

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    Phenytoin (Isoproterenol, ISO,  1 mg/kg, i.p.). The typical ECG recording showing that ageing increased susceptibility to ISO-induced arrhythmias using PES with PCL from 90 ms to 30 ms.

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    Histogram of the VT occurrence rate in young and old mice with or without treatment of Phenytoin (Isoproterenol, ISO, 1  mg/kg, i.p.) (∗∗P < 0.01 and ∗∗∗P < 0.001).

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    The typical APD80 map and AP trace (young: black line; old: red line) in intact Langendorff perfused young and old mouse hearts before and following Phenytoin (Isoproterenol, ISO,  1μM) challenge in pacing at 10 Hz.

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    Quantitative analysis of APD80 from individual hearts in young and old mice before and following Phenytoin (Isoproterenol, ISO,  1μM) (∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001).

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    Ageing induced fractional increases in APD80 challenged by Phenytoin (Isoproterenol, ISO,  1μM) (ΔAPD80) (P > 0.05).

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    The typical CaTD50 map and calcium transient trace (young: black line; old: red line) in intact Langendorff perfused young and old mouse hearts before and following Phenytoin (Isoproterenol, ISO,  1μM) challenge in pacing at 10 Hz.

    Phenytoin sodium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 28;2022:8538296.  [Abstract]

    Histogram showing the τ30–90 in young and old mice before and following Phenytoin (Isoproterenol, ISO,  1μM) pacing at 10 Hz (∗P < 0.05 and ∗∗P < 0.01). n = 5 (each group).

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    製品説明

    Phenytoin sodium (5,5-Diphenylhydantoin sodium salt) is a potent Voltage-gated Na+ channels (VGSCs) blocker. Phenytoin has antiepileptic activity and reduces breast tumour growth and metastasis in mice[1][2].

    体外実験

    Phenytoin sodium is an antiepileptic agent. It is useful to treat partial seizures and generalized tonic-clonic seizures but not primary generalized seizures such as absence seizures or myoclonic seizures. Phenytoin is believed to protect against seizures by causing voltage-dependent block of voltage-gated sodium channels[2].
    Phenytoin has low affinity for resting sodium channels at hyperpolarized membrane potentials[3].
    When neurons are depolarized and the channels transition into the open and inactivated states, greater binding and block occur. The inhibitory potency is strongly use dependent, so that block accumulates with prolonged or repetitive activation, such as occurs during a seizure discharge. The blocking of sodium channels by phenytoin is of slow onset. The time course of fast sodium currents is therefore not altered in the presence of the drug and action potentials evoked by synaptic depolarizations of ordinary duration are not blocked. Thus phenytoin is able to selectively inhibit pathological hyperexcitability in epilepsy without unduly impairing ongoing activity. Phenytoin also blocks persistent sodium current and this may be of particular importance in seizure control. Phenytoin is a class 1b antiarrhythmic[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Phenytoin (sodium) 関連抗体
    体内実験

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment    .

    Phenytoin (60 mg/kg; once daily for 28 days) sodium reduces MDA-MB-231 cells in six-week-old female Rag2-/- Il2rg-/- mice[1].
    Phenytoin can induce a model of gingival hyperplasia in mice[6].

    Induction of Gingival Hyperplasia in Mice[5]
    Background
    Phenytoin induced gingival overgrowth, a side effect with multifactorial aetiology, is characterized by an increase in the volume of extracellular tissues, particularly collagenous components, with varying degrees of inflammation.
    Specific Modeling Methods
    Mice: BALB/cByJ • male • 8 week old
    Administration: 30 mg/kg • miniosmotic pumps • 0.5-μL/h • 2 weeks
    Note
    (1) Mice are housed for 1 week before any procedure for acclimation.
    (2) Pumps are replaced every 2 weeks throughout the 12-week experimental period.
    (3) Mice are then euthanized and heads are immediately placed in freshly made 4% paraformaldehyde for 24 hours for fixation.
    Modeling Indicators
    Morphology: Gingival overgrowth was apparent in the maxillary anterior gingival tissue of mice; Increased the papillary height and area.
    Histomorphometry: Increased epithelial thickness and area in the maxillary anterior zone.
    Molecular changes: Ccn2 is overexpressed in all regions of the epithelium; TGF-β1 and LOXL2 is overexpressed in both epithelial and connective tissues.
    Correlated Product(s): Cyclosporine A (HY-B0579)
    Opposite Product(s): Lovastatin (HY-N0504)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    274.25

    分子式

    C15H11N2NaO2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1N=C(O[Na])NC1(C2=CC=CC=C2)C3=CC=CC=C3
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (182.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6463 mL 18.2315 mL 36.4631 mL
    5 mM 0.7293 mL 3.6463 mL 7.2926 mL
    10 mM 0.3646 mL 1.8232 mL 3.6463 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

    V1

    =
    濃度 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.78%

    COA (259 KB) HNMR (254 KB) LCMS (90 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6463 mL 18.2315 mL 36.4631 mL 91.1577 mL
    5 mM 0.7293 mL 3.6463 mL 7.2926 mL 18.2315 mL
    10 mM 0.3646 mL 1.8232 mL 3.6463 mL 9.1158 mL
    15 mM 0.2431 mL 1.2154 mL 2.4309 mL 6.0772 mL
    20 mM 0.1823 mL 0.9116 mL 1.8232 mL 4.5579 mL
    25 mM 0.1459 mL 0.7293 mL 1.4585 mL 3.6463 mL
    30 mM 0.1215 mL 0.6077 mL 1.2154 mL 3.0386 mL
    40 mM 0.0912 mL 0.4558 mL 0.9116 mL 2.2789 mL
    50 mM 0.0729 mL 0.3646 mL 0.7293 mL 1.8232 mL
    60 mM 0.0608 mL 0.3039 mL 0.6077 mL 1.5193 mL
    80 mM 0.0456 mL 0.2279 mL 0.4558 mL 1.1395 mL
    100 mM 0.0365 mL 0.1823 mL 0.3646 mL 0.9116 mL
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    Phenytoin sodium Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Phenytoin630-93-35,5-DiphenylhydantoinSodium ChannelVirus ProteaseNa channelsNa+ channelsVoltage-gatedNa+VGSCsantiepilepticbreasttumourmetastasisInhibitorinhibitorinhibit

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    製品名:
    Phenytoin sodium
    製品番号:
    HY-B0448A
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