Bruceine D

Name: Bruceine D
Brand: MedChemExpress
SKU: HY-N3014
Rating: 5.0 (4 reviews)

Cat. No.: HY-N3014 Pureté: 99.50%: Fiche technique
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SDS
Instruction de manipulation
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Bruceine D is a Notch inhibitor with anti-cancer activity and induces apoptosis in several human cancer cells. Bruceine D is an effective botanical insect antifeedant with outstanding systemic properties, causing potent pest growth inhibitory activity. Bruceine D has strong anthelmintic activity against D. intermedius with an EC₅₀ value of 0.57 mg/L.

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CAS No. : 21499-66-1

Size	Stock	Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtenir un devis
500 mg	Obtenir un devis

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Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE Bruceine D

Cell Proliferation/Viability Assay

Flow Cytometry

Apoptosis Analysis

: Bruceine D purchased from MedChemExpress. Usage Cited in: ACS Omega. 2022 Aug 5;7(32):28334-28341.; MTT assays performed to measure the anti-proliferative potency of Bruceine D and phorbol 12-myristate 13-acetate against MDA-MB-231 cell.

: Bruceine D purchased from MedChemExpress. Usage Cited in: ACS Omega. 2022 Aug 5;7(32):28334-28341.; Immunofluorescence confocal microscopy images of MDA-MB-231 cells treated with 10 μM Bruceine D and 10 μM phorbol 12-myristate 13-acetate for 24 h, respectively.

: Bruceine D purchased from MedChemExpress. Usage Cited in: ACS Omega. 2022 Aug 5;7(32):28334-28341.; MDA-MB-231 cell treated with 10 μM Bruceine D and 10 μM phorbol 12-myristate 13-acetate for 24 h, respectively. Cell cycle analysis was performed with propidium iodide.

: Bruceine D purchased from MedChemExpress. Usage Cited in: ACS Omega. 2022 Aug 5;7(32):28334-28341.; Western blot analysis of CDK1, CDK2, and cyclin E in MDA-MB-231 cell treated with 10 μM Bruceine D and 10 μM phorbol 12-myristate 13-acetate for 24 h, respectively. Relative CDK1, CDK2, and cyclin E expression levels were quantified by normalization to β-actin.

: Bruceine D purchased from MedChemExpress. Usage Cited in: ACS Omega. 2022 Aug 5;7(32):28334-28341.; MDA-MB-231 cells were treated with 10 μM Bruceine D and 10 μM phorbol 12-myristate 13-acetate for 24 h, respectively. Apoptosis ratios were determined by flow cytometry analysis of annexin V/PI double staining.

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Description

Bruceine D is a Notch inhibitor with anti-cancer activity and induces apoptosis in several human cancer cells. Bruceine D is an effective botanical insect antifeedant with outstanding systemic properties, causing potent pest growth inhibitory activity^[1]^[2]. Bruceine D has strong anthelmintic activity against D. intermedius with an EC₅₀ value of 0.57 mg/L^[3].

IC₅₀ & Target

Notch^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	19 μM Compound: 9j	Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]
MDA-MB-231	IC₅₀	24 μM Compound: 9j	Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]
PANC-1	IC₅₀	1.3 μM Compound: 9j	Cytotoxicity against human Panc-1 cells incubated for 72 hrs by sulphorhodamine B assay Cytotoxicity against human Panc-1 cells incubated for 72 hrs by sulphorhodamine B assay	[PMID: 33289552]
SW1990	IC₅₀	2.1 μM Compound: 9j	Antiproliferative activity against human SW1990 incubated for 72 hrs by sulphorhodamine B assay Antiproliferative activity against human SW1990 incubated for 72 hrs by sulphorhodamine B assay	[PMID: 33289552]

In Vitro

Bruceine D inhibits the cell viability of H460 and A549 cells in a dose-dependent manner with IC₅₀ values of 0.5 and 0.6?μM, respectively, at 48?h of treatment^[1].
? Bruceine D (0.125-1.0?μM) dose-dependently promotes chromatin condensation, Annexin V-positive cell population and caspase-dependent apoptosis in H460 and A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

410.42

Formule

C₂₀H₂₆O₉

CAS No.

21499-66-1

Appearance

Solid

Color

White to off-white

SMILES

O[C@@]1([C@H]2O)[C@@]34[C@@]([C@@H](O)[C@H](O)[C@]1(OC4)C)([H])[C@]([C@@](C(C)=CC5=O)([H])C[C@@]3([H])OC2=O)([C@@H]5O)C

Structure Classification

Initial Source

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (243.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4365 mL	12.1826 mL	24.3653 mL
5 mM	0.4873 mL	2.4365 mL	4.8731 mL
10 mM	0.2437 mL	1.2183 mL	2.4365 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.50%

Select Batch:

Fiche technique (283 KB) SDS (393 KB)

COA (259 KB) HNMR (137 KB) CNMR (144 KB) RP-HPLC (226 KB) MS (168 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Mao G,et al. Bruceine D Isolated from Brucea Javanica (L.) Merr. as a Systemic Feeding Deterrent for Three Major Lepidopteran Pests. J Agric Food Chem. 2019 Apr 17;67(15):4232-4239. [Content Brief]

[2]. Cheng Z, et al. Bruceine D inhibits hepatocellular carcinoma growth by targeting β-catenin/jagged1 pathways. Cancer Lett. 2017 Sep 10;403:195-205. [Content Brief]

[3]. Yong Wang, et al. In vivo anthelmintic activity of bruceine A and bruceine D from Brucea javanica against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Vet Parasitol. 2011 Apr 19;177(1-2):127-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4365 mL	12.1826 mL	24.3653 mL	60.9132 mL
	5 mM	0.4873 mL	2.4365 mL	4.8731 mL	12.1826 mL
	10 mM	0.2437 mL	1.2183 mL	2.4365 mL	6.0913 mL
	15 mM	0.1624 mL	0.8122 mL	1.6244 mL	4.0609 mL
	20 mM	0.1218 mL	0.6091 mL	1.2183 mL	3.0457 mL
	25 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4365 mL
	30 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0304 mL
	40 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	50 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2183 mL
	60 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7614 mL
	100 mM	0.0244 mL	0.1218 mL	0.2437 mL	0.6091 mL

Bruceine D Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bruceine D21499-66-1NotchApoptosisParasiteInhibitorinhibitorinhibit

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Bruceine D

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Bruceine D Related Antibodies

4 Publications Citing Use of MCE Bruceine D

Recommandations en vedette

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Parasite:

Activité biologique

Pureté et documentation

Références

Avis client

Bruceine D Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Bruceine D Related Classifications

Keywords:

Vos produits récemment consultés:

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