Daptomycin (Synonyms: LY146032)

Name: Daptomycin
Brand: MedChemExpress
SKU: HY-B0108
Rating: 5.0 (25 reviews)

製品番号: HY-B0108 純度: 99.55%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Daptomycin is a lipopeptide antibiotic with rapid in vitro bactericidal activity against gram-positive organisms.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

カスタムペプチド

CAS 番号 : 103060-53-3

容量	価格（税別）	在庫状況	数量
>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
5 mg	$30	在庫あり	Estimated Time of Arrival: December 31
10 mg	$45	在庫あり	Estimated Time of Arrival: December 31
25 mg	$78	在庫あり	Estimated Time of Arrival: December 31
50 mg	$119	在庫あり	Estimated Time of Arrival: December 31
100 mg	$217	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 25 publication(s) in Google Scholar

Other Forms of Daptomycin:

Daptomycin-d₅ TFA お問い合わせ
Daptomycin-d₅ お問い合わせ

顧客検証

Bio/Physico-chemical Assay

RT-PCR

Microbiological Assay

Cell Imaging/Staining

: Daptomycin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Sep 30:e0212425. [Abstract]; Kupffer cell metabolism upon Daptomycin exposure in the absence of infection. KCs were treated with antibiotics at reported human Cmax concentrations for 10 h. M1 (Il6, Tnf, and Nos2) and M2 (Il10 and Arg1) polarization, as well as metabolic genes (Irg1, Hif1α, and Sdhb) were assessed by RT-qPCR.

: Daptomycin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Sep 30:e0212425. [Abstract]; Kupffer cell metabolism upon Daptomycin exposure in the absence of infection. NAD(P)H fluorescence lifetime was determined by TPE-FLIM.

: Daptomycin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Sep 30:e0212425. [Abstract]; Kupffer cell metabolism upon Daptomycin exposure in the absence of infection. Fluorescence decay curves from generated NAD(P)H data acquired by TPE-FLIM from each region of interest were fitted to a biexponential decay function and exported to ImageJ for single-cell NAD(P)H lifetime analysis. Spatial NAD(P)H lifetime data were further processed, then overlaid with associated transmitted light images to generate representative FLIM images for each treatment group.

: Daptomycin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 May 2;15(1):15346.; Population analysis profiles of MRSA isolates XF1 and XF2 in the presence of daptomycin.

: Daptomycin purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Oct 23:e0157625. [Abstract]; Twenty-four-hour survival curves of six MRSA isolates and one MSSA isolate (STA 29213) at mid-exponential phase treated with steady-state concentrations of daptomycin (DAP, 0.5-1 mg/L). Each time point represents the mean values of the two replicates with standard deviations.

Antibiotic アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Daptomycin is a lipopeptide antibiotic with rapid in vitro bactericidal activity against gram-positive organisms.

IC₅₀ & Target

Lipopeptide

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	CC₅₀	> 100 3 Compound: Daptomycin	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue reporter assay	[PMID: 30392371]
MDCK	EC₅₀	0.18 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
MDCK	EC₅₀	0.62 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs	[PMID: 17548493]
MDCK	EC₅₀	0.7 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
MDCK	EC₅₀	2.67 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 at 1 mg/liter after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 at 1 mg/liter after 24 hrs	[PMID: 17548493]
THP-1	EC₅₀	0.27 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 0.5 uM elacridar Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 0.5 uM elacridar	[PMID: 17548493]
THP-1	EC₅₀	0.31 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
THP-1	EC₅₀	1.14 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs	[PMID: 17548493]
MDCK	EC₅₀	0.18 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
MDCK	EC₅₀	0.62 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in wild type MDCK cells after 24 hrs	[PMID: 17548493]
MDCK	EC₅₀	0.7 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
MDCK	EC₅₀	2.67 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 at 1 mg/liter after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in MDCK cells over expressing human MADR1 at 1 mg/liter after 24 hrs	[PMID: 17548493]
THP-1	EC₅₀	0.27 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 0.5 uM elacridar Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 0.5 uM elacridar	[PMID: 17548493]
THP-1	EC₅₀	0.31 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 100 uM verapamil Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs in presence of 100 uM verapamil	[PMID: 17548493]
THP-1	EC₅₀	1.14 10 Compound: Daptomycin	Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs Antibacterial activity against methicillin-sensitive Staphylococcus aureus ATCC 25923 phagocytized in human THP1 cells after 24 hrs	[PMID: 17548493]

体外実験

Daptomycin has excellent in-vitro inhibitory and bactericidal activity against nafcillin-susceptible and resistant staphylococci (MIC₉₀ less than or equal to 0.5 mg/L) and against enterococci (MIC₉₀ less than or equal to 2.0 mg/L). Daptomycin is more active than vancomycin against the majority of isolated tested. With the exception of trimethoprim-sulphamethoxazole, daptomycin is the most active agent in vitro against enterococci, and is the most active against nafcillin-resistant staphylococci. Daptomycin and vancomycin show a marked increase in MIC when the inoculum is increased from 10⁵ to 10⁷ cfu/mL^[1]. Daptomycin is effective within a very narrow range of drug concentrations (from 0.125 to 2.0 tLg/mL) and is more active than other agents tested against S. faecalis^[2]. Daptomycin inhibits the formation of these nucleotide-linked intermediates^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

At a dose of 10 mg/kg given twice daily, daptomycin reduces the number of organisms per kidney significantly compared with that in infected untreated controls within 48 h after the initiation of therapy. At 20 mg/kg given once a day, daptomycin is less effective but reduces colony counts significantly after 4 days of therapy, and its activity is comparable to that of vancomycin or vancomycin-gentamicin given twice daily^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

1620.67

分子式

C₇₂H₁₀₁N₁₇O₂₆

CAS 番号

103060-53-3

Appearance

Solid

Color

Off-white to light yellow

Sequence

Dec-Trp-{d-Asn}-Asp-Thr-Gly-{Orn}-Asp-{d-Ala}-Asp-Gly-{d-Ser(Me)}-Glu-{Kynurenine} (Lactone: Thr4-Kynurenine13)

Sequence Shortening

Dec-W-{d-Asn}-DTG-{Orn}-D-{d-Ala}-DG-{d-Ser(Me)}-E-{Kynurenine} (Lactone: Thr4-Kynurenine13)

Structure Classification

Initial Source

Microorganisms

soil actinomycete

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Sealed storage, away from moisture
Powder	-80°C	2 years
	-20°C	1 year
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

H₂O : 100 mg/mL (61.70 mM; Need ultrasonic)

DMSO : ≥ 100 mg/mL (61.70 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6170 mL	3.0851 mL	6.1703 mL
5 mM	0.1234 mL	0.6170 mL	1.2341 mL
10 mM	0.0617 mL	0.3085 mL	0.6170 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (1.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (1.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (1.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (61.70 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.55%

Select Batch:

データシート (284 KB) SDS (396 KB)

COA (248 KB) HNMR (140 KB) RP-HPLC (119 KB) LCMS (232 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Benson CA, et al. Comparative in-vitro activity of LY146032 a new peptolide, with vancomycin and eight other agents against gram-positive organisms. J Antimicrob Chemother. 1987 Aug;20(2):191-6. [Content Brief]

[2]. Miniter PM, et al. Activity of LY146032 in vitro and in experimental enterococcal pyelonephritis. Antimicrob Agents Chemother. 1987 Aug;31(8):1199-203. [Content Brief]

[3]. Allen NE, et al. Inhibition of peptidoglycan biosynthesis in gram-positive bacteria by LY146032. Antimicrob Agents Chemother. 1987 Jul;31(7):1093-9. [Content Brief]

動物実験
^[2]

Inocula containing 109 CFU/mL are prepared from an 18-h brain heart infusion broth culture. The exact number in each inoculum is subsequently determined by the standard serial 10-fold dilution agar pour plate technique. Animals are challenged intravenously with a 1.0-mL inoculum. This inoculum is known to infect the renal medulla of normal rats. Twenty-four hours later the animals are divided into eight groups and are given either saline only (controls) or antibiotic therapy initiated with Daptomycin at 10 mg/kg (Daptomycin10), Daptomycin at 20 mg/kg (Daptomycin20), Daptomycin10 plus gentamicin at 1.5 mg/kg, vancomycin at 20 mg/kg, vancomycin at 20 mg/kg plus gentamicin at 1.5 mg/kg, ampicillin at 30 mg per rat per injection, and ampicillin at 30 mg per rat plus gentamicin at 1.5 mg/kg. Animals receive Daptomycin20 once daily; all other drugs are administered twice daily. Vancomycin and Daptomycin are given subcutaneously; ampicillin and gentamicin are given intramuscularly. Drugs are administered for up to 13 days.

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Benson CA, et al. Comparative in-vitro activity of LY146032 a new peptolide, with vancomycin and eight other agents against gram-positive organisms. J Antimicrob Chemother. 1987 Aug;20(2):191-6. [Content Brief]

[2]. Miniter PM, et al. Activity of LY146032 in vitro and in experimental enterococcal pyelonephritis. Antimicrob Agents Chemother. 1987 Aug;31(8):1199-203. [Content Brief]

[3]. Allen NE, et al. Inhibition of peptidoglycan biosynthesis in gram-positive bacteria by LY146032. Antimicrob Agents Chemother. 1987 Jul;31(7):1093-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.6170 mL	3.0851 mL	6.1703 mL	15.4257 mL
	5 mM	0.1234 mL	0.6170 mL	1.2341 mL	3.0851 mL
	10 mM	0.0617 mL	0.3085 mL	0.6170 mL	1.5426 mL
	15 mM	0.0411 mL	0.2057 mL	0.4114 mL	1.0284 mL
	20 mM	0.0309 mL	0.1543 mL	0.3085 mL	0.7713 mL
	25 mM	0.0247 mL	0.1234 mL	0.2468 mL	0.6170 mL
	30 mM	0.0206 mL	0.1028 mL	0.2057 mL	0.5142 mL
	40 mM	0.0154 mL	0.0771 mL	0.1543 mL	0.3856 mL
	50 mM	0.0123 mL	0.0617 mL	0.1234 mL	0.3085 mL
	60 mM	0.0103 mL	0.0514 mL	0.1028 mL	0.2571 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Daptomycin Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Daptomycin103060-53-3LY146032LY 146032LY-146032BacterialAntibioticInhibitorinhibitorinhibit

日本の代理先をお選びくださいナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
製品名			カスタマ需要量 *
お名前 *			タイトル
メールアドレス *			電話番号 *
デパートメント			組纖名 *
市区町村			都道府県
国或いは地域 *
必ず会社名を記載ください。個人への返信は行いません。
備考

製品名			Lot #
お名前 *			メールアドレス *
電話番号 *			部門 *
組纖名 *			国或いは地域 *
備考

Daptomycin (Synonyms: LY146032)

カスタマーレビュー

Other Forms of Daptomycin:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Antibiotic アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Daptomycin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

Daptomycin (Synonyms: LY146032)

カスタマーレビュー

Other Forms of Daptomycin:

Daptomycin 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Antibiotic アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Daptomycin 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Daptomycin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report