2. Membrane Transporter/Ion Channel Neuronal Signaling Anti-infection
  3. Dengue Virus TRP Channel Flavivirus
  4. ML-SA1

ML-SA1, as a selective TRPML agonist, inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 value of ML-SA1 against DENV2 RNA and ZIKV RNA is 8.3 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

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CAS No. : 332382-54-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg   견적 받기  
200 mg   견적 받기  

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고객리뷰

Based on 9 publication(s) in Google Scholar

Other Forms of ML-SA1:

ML-SA1 Related Antibodies

Top Publications Citing Use of Products

    ML-SA1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 25:e12407.  [Abstract]

    Representative images of Fluo 8‐AM staining (arrowheads) in HGC‐27 cells stably transfected withscramble shRNA (sh‐Scb), sh‐PITX2 #1, or sh‐PITX2 #2 under SD condition, and those treated with ML‐SA1 (20 µM), with nuclei staining by DAPI.

    ML-SA1 purchased from MedChemExpress. Usage Cited in: Engineering. 2025 Nov 14.

    ML-SA1 (20 μM; 24 h). Lv-CMV-Mito-Gcamp6f-WPRE was used to measure mitochondrial Ca2+ in NMCMs after treatment with Ang II and ML-SA1 for 24 h.

    ML-SA1 purchased from MedChemExpress. Usage Cited in: Engineering. 2025 Nov 14.

    ML-SA1 (20 μM; 24 h). Western blot analysis of Mfn2, OPA1, total-dynamin-related protein 1 (T-Drp1), p-Drp1Ser637, and p-Drp1Ser616 expression in NMCMs treated with Ang II and ML-SA1 for 24 h.

    ML-SA1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Apr;19(4):1128-1143.  [Abstract]

    ML-SA1 (20 nM; 10 days). The abundance of RBSDV, Atg8, and ref(2)P/SQTSM1 was analyzed by western blotting.

    ML-SA1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Apr;19(4):1128-1143.  [Abstract]

    ML-SA1 inhibited the lysosome-autophagosome fusion in Sf9 cells. Sf9 cells were infected with RFP-eGFP-Atg8 bacmids for 6 h, treated with Rapamycin (20 μM) for 6 h, and then incubated with DMSO or ML-SA1 (10 nM) for 24 h. The cells were analyzed by confocal microscopy.

    View All TRP Channel Isoform Specific Products:

    View All Isoforms
    TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    ML-SA1, as a selective TRPML agonist, inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 value of ML-SA1 against DENV2 RNA and ZIKV RNA is 8.3 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral[1].

    IC50 & Target

    IC50: 8.3 μM (DENV2)[1].
    IC50: 52.99 μM (ZIKV)[1]
    In Vitro

    ML-SA1 (25 μM; 0~14 hours; A549 cells) possibly affects the entry of DENV2 into host cells[1].
    ML-SA1 (0~200 μM; A549 cells) shows that there is no cytotoxicity to the cell line observed, even at concentrations up to 200 μM. ML-SA1 (0~50 μM; A549 cells) significantly suppresses DENV2 at the RNA levels and the IC50 is 8.93 μM[1]. ML-SA1 results in a dose-dependent decrease in ZIKV in A549 cells at both the RNA and protein levels, and the IC50 value of ML-SA1 against ZIKV RNA is 52.99 μM. ML-SA1, as an activator of TRPMLs, appears to be a potent inhibitor of DENV2 and ZIKV in vitro. ML-SA1 promotes lysosomal acidification and protease activity to inhibit viral infection. ML-SA1 can induce autophagy in Huh7 cells or A549 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML-SA1 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: A549 cells
    Concentration: 25 μM
    Incubation Time: 0~14 hours
    Result: Possibly affected the entry of DENV2 into host cells.
    분자량

    362.42

    화학식

    C22H22N2O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1N(CC(N2C(C)(C)CC(C)C3=C2C=CC=C3)=O)C(C4=C1C=CC=C4)=O
    선적

    Room temperature in continental US; may vary elsewhere.
    보관
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    용액&용해도
    In Vitro: 

    DMSO : 20.83 mg/mL (57.47 mM; ultrasonic and warming and heat to 65°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7592 mL 13.7961 mL 27.5923 mL
    5 mM 0.5518 mL 2.7592 mL 5.5185 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
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    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    순도&문서
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7592 mL 13.7961 mL 27.5923 mL 68.9807 mL
    5 mM 0.5518 mL 2.7592 mL 5.5185 mL 13.7961 mL
    10 mM 0.2759 mL 1.3796 mL 2.7592 mL 6.8981 mL
    15 mM 0.1839 mL 0.9197 mL 1.8395 mL 4.5987 mL
    20 mM 0.1380 mL 0.6898 mL 1.3796 mL 3.4490 mL
    25 mM 0.1104 mL 0.5518 mL 1.1037 mL 2.7592 mL
    30 mM 0.0920 mL 0.4599 mL 0.9197 mL 2.2994 mL
    40 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7245 mL
    50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3796 mL
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    ML-SA1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML-SA1332382-54-4Dengue VirusTRP ChannelFlavivirusDENVTransient receptor potential channelsselectiveDenguevirusZikalysosomalacidificationproteaseautophagybroad-spectrumantiviralInhibitorinhibitorinhibit

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