2. Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Endogenous Metabolite Apoptosis Parasite
  4. 5'-Methylthioadenosine

5'-Methylthioadenosine  (Synonyms: 5'-(Methylthio)-5'-deoxyadenosine; 5'-Deoxy-5'-(methylthio)adenosine; 5'-S-Methyl-5'-thioadenosine)

Cat. No.: HY-16938 Pureté: 99.75%
COA
SDS
Instruction de manipulation Technical Support

5'-Methylthioadenosine (5'-(Methylthio)-5'-deoxyadenosine) is a nucleoside generated from S-adenosylmethionine (SAM) during polyamine synthesis. 5'-Methylthioadenosine suppresses tumors by inhibiting tumor cell proliferation, invasion, and the induction of apoptosis while controlling the inflammatory micro-environments of tumor tissue. 5'-Methylthioadenosine and its associated materials have striking regulatory effects on tumorigenesis.

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CAS No. : 2457-80-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
25 mg En stock
50 mg En stock
100 mg En stock
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Based on 8 publication(s) in Google Scholar

Other Forms of 5'-Methylthioadenosine:

5'-Methylthioadenosine Related Antibodies

Top Publications Citing Use of Products

    5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Feb 11;58(2):381-396.e9.  [Abstract]

    BMDMs were treated or not with 5'-Methylthioadenosine (MTA, 500 μM) 1 h before plating in trehalose-6,6-dibehenate (TDB)-coated wells. After 24 h, cells were re-plated in non-TDB-coated plates and rested for 5 days before stimulation with CpG for 24 h. TNF (left) and IL-6 (right) production measured by ELISA in culture supernatant of BMDMs from indicated treatments and stimulations.

    5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: J Mol Cell Cardiol. 2023 Jan:174:88-100.  [Abstract]

    qPCR analysis of the mRNA level of Irf4 in BMDMs pretreated with medium or 5'-Methylthioadenosine (MTA, 1 mM) after IL-4 (10 ng/mL) treatment for 6 h. n = 3–4 per group.

    5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: J Mol Cell Cardiol. 2023 Jan:174:88-100.  [Abstract]

    Immunoblot analysis of IRF4 and H3K4me3 levels in BMDMs pretreated with medium or 5'-Methylthioadenosine (MTA, 1 mM) after IL-4 (10 ng/mL) treatment for 24 h.

    5'-Methylthioadenosine purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2020 Feb;35(2):277-291.  [Abstract]

    The expression of H3K4me3 and apoptosis-related genes in cocultured SD rat Sertoli-germ cells after exposed to 0, 9, 18, 36 (μg/mL) MC-LR or MC-LR (36 μg/mL) + 5'-Methylthioadenosine (MTA, 1 mmoL/L). The mRNA levels of apoptosis-related genes in the apoptosis signaling pathway.

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    Description

    5'-Methylthioadenosine (5'-(Methylthio)-5'-deoxyadenosine) is a nucleoside generated from S-adenosylmethionine (SAM) during polyamine synthesis[1]. 5'-Methylthioadenosine suppresses tumors by inhibiting tumor cell proliferation, invasion, and the induction of apoptosis while controlling the inflammatory micro-environments of tumor tissue. 5'-Methylthioadenosine and its associated materials have striking regulatory effects on tumorigenesis[2].

    IC50 & Target

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CCRF-CEM IC50
    150 μM
    Compound: MTA
    The concentration required to inhibit cell growth by 50% was measured on human CCRF-CEM cells (96h after exposure)
    The concentration required to inhibit cell growth by 50% was measured on human CCRF-CEM cells (96h after exposure)
    		[PMID: 1908523]
    L1210 IC50
    600 μM
    Compound: 3
    Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L1210 murine leukemia cells
    Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L1210 murine leukemia cells
    		[PMID: 2496231]
    L1210 IC50
    850 μM
    Compound: MTA
    The concentration required to inhibit cell growth by 50% was measured on L1210 Leukemic cell line of mouse(48h after exposure)
    The concentration required to inhibit cell growth by 50% was measured on L1210 Leukemic cell line of mouse(48h after exposure)
    		[PMID: 1908523]
    L5178Y IC50
    200 μM
    Compound: MTA
    The concentration required to inhibit cell growth by 50% was measured on L5178Y Leukemic cell line of mouse(48h after exposure)
    The concentration required to inhibit cell growth by 50% was measured on L5178Y Leukemic cell line of mouse(48h after exposure)
    		[PMID: 1908523]
    L5178Y IC50
    60 μM
    Compound: 3
    Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L5178Y murine leukemia cells
    Compound was evaluated for its ability to inhibit growth by 50% at 48 hr in L5178Y murine leukemia cells
    		[PMID: 2496231]
    MOLT-4 IC50
    11 μM
    Compound: MTA
    The concentration required to inhibit cell growth by 50% was measured on human MOLT-4 cells (96h after exposure)
    The concentration required to inhibit cell growth by 50% was measured on human MOLT-4 cells (96h after exposure)
    		[PMID: 1908523]
    In Vitro

    5'-Methylthioadenosine protects MTAP+ cells significantly better than MTAP cells from 6TG (6′-thioguanine) toxicity[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    5'-Methylthioadenosine Related Antibodies

    Cell Viability Assay[3]

    Cell Line: MTAP+ and MTAP− NIH3T3 cells, isogenic MTAP+ and MTAP− cell line derived from a human HT1080 fibrosarcoma cell line
    Concentration: 6TG and 2FA in combination with 10 μM MTA
    Incubation Time: 48 hours
    Result: Significantly increased IC50 concentration in MTAP+ cells, but no significant change in MTAP− cells.
    In Vivo

    The 2FA (2′-fluoroadenine) + MTA (5'-Methylthioadenosine) combination (100 mg/kg MTA; 20 mg/kg 2FA) inhibits tumor growth of four different MTAP human tumor cell lines in mouse xenograft models[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Masse moléculaire

    297.33

    Formule

    C11H15N5O3S
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CSC)O3
    Structure Classification
    Initial Source
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvant et solubilité
    In Vitro: 

    DMSO : 100 mg/mL (336.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3633 mL 16.8163 mL 33.6327 mL
    5 mM 0.6727 mL 3.3633 mL 6.7265 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation

    Purity: 99.75%

    SDS (394 KB)

    COA (253 KB) HNMR (132 KB) RP-HPLC (87 KB) MS (70 KB)

    Instruction de manipulation (2659 KB)
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3633 mL 16.8163 mL 33.6327 mL 84.0817 mL
    5 mM 0.6727 mL 3.3633 mL 6.7265 mL 16.8163 mL
    10 mM 0.3363 mL 1.6816 mL 3.3633 mL 8.4082 mL
    15 mM 0.2242 mL 1.1211 mL 2.2422 mL 5.6054 mL
    20 mM 0.1682 mL 0.8408 mL 1.6816 mL 4.2041 mL
    25 mM 0.1345 mL 0.6727 mL 1.3453 mL 3.3633 mL
    30 mM 0.1121 mL 0.5605 mL 1.1211 mL 2.8027 mL
    40 mM 0.0841 mL 0.4204 mL 0.8408 mL 2.1020 mL
    50 mM 0.0673 mL 0.3363 mL 0.6727 mL 1.6816 mL
    60 mM 0.0561 mL 0.2803 mL 0.5605 mL 1.4014 mL
    80 mM 0.0420 mL 0.2102 mL 0.4204 mL 1.0510 mL
    100 mM 0.0336 mL 0.1682 mL 0.3363 mL 0.8408 mL
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    5'-Methylthioadenosine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5'-Methylthioadenosine2457-80-95'-(Methylthio)-5'-deoxyadenosine 5'-Deoxy-5'-(methylthio)adenosine 5'-S-Methyl-5'-thioadenosineEndogenous MetaboliteApoptosisParasiteMTASAMcancerfluoroadenineNIH3T3nucleosideMTAP?MTAP+Inhibitorinhibitorinhibit

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