2. Immunology/Inflammation Neuronal Signaling GPCR/G Protein MAPK/ERK Pathway Stem Cell/Wnt
  3. COX Melanocortin Receptor ERK
  4. Fenoprofen Calcium hydrate

Fenoprofen Calcium hydrate  (Synonyms: LILLY-53858 Calcium hydrate)

Cat. No.: HY-B0288B Pureza: 99.52%
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Fenoprofen (LILLY-53858) Calcium hydrate is a nonsteroidal anti-inflammatory agent (NSAID) and inhibits cyclooxygenase (COX). Fenoprofen Calcium hydrate is a melanocortin receptors (MCRs) positive allosteric modulator (PAM). Fenoprofen Calcium hydrate also increases ERK1/2 activation in HEK293T cells. Fenoprofen Calcium hydrate has anti-arthritic activities and can be used for the study of rheumatoid arthritis and osteoarthritis.

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Fenoprofen Calcium hydrate

Fenoprofen Calcium hydrate Estructura química

No. CAS : 71720-56-4

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
500 mg En stock
1 g En stock
5 g En stock
10 g   Obtener un presupuesto  
50 g   Obtener un presupuesto  

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Revisión del cliente

Based on 2 publication(s) in Google Scholar

Other Forms of Fenoprofen Calcium hydrate:

Fenoprofen Calcium hydrate Related Antibodies

Top Publications Citing Use of Products

Ver todos los productos específicos de isoformas COX:

Ver todas las isoformas
COX COX-1 COX-2 COX-3

Ver todos los productos específicos de isoformas Melanocortin Receptor:

Ver todas las isoformas
MC1R MC3R MC2R MC4R MC5R

Ver todos los productos específicos de isoformas ERK:

Ver todas las isoformas
ERK ERK1 ERK2 ERK5 ERK8

  • Actividad biológica

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Descripciòn

Fenoprofen (LILLY-53858) Calcium hydrate is a nonsteroidal anti-inflammatory agent (NSAID) and inhibits cyclooxygenase (COX). Fenoprofen Calcium hydrate is a melanocortin receptors (MCRs) positive allosteric modulator (PAM). Fenoprofen Calcium hydrate also increases ERK1/2 activation in HEK293T cells. Fenoprofen Calcium hydrate has anti-arthritic activities and can be used for the study of rheumatoid arthritis and osteoarthritis[1][2].

IC50 & Target[1]

COX

 

In Vitro

In HEK293T cells, Fenoprofen Calcium hydrate stimulates ERK1/2 activation at MC3-5R in a biphasic manner, with the highest ERK1/2 activation observed at concentrations of 3 and 10 µM for MC3R, 1 µM for MC4R, and 3 µM for MC5R. No significant difference of Fenoprofen Calcium hydrate-induced pERK1/2 levels was observed in cells transfected with the empty vector pcDNA3.1 as well as MC1R. Fenoprofen Calcium hydrate induces biased signaling at MC3-5R, as it selectively activated ERK1/2 cascade but not the canonical cAMP signaling[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fenoprofen Calcium hydrate Related Antibodies
In Vivo

Fenoprofen (10 mg/kg; i.p.; twice per day from day 2; for 7 days) Calcium hydrate presents anti-arthritic actions on cartilage integrity and synovitis, effects markedly attenuated in Mc3r-/- mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6J wild-type (WT) mice (7-8 weeks old) and Mc3r-/- mice injected with the K/BxN serum[2]
Dosage: 10 mg/kg
Administration: i.p.; twice per day from day 2; for 7 days
Result: Reduced paw volume and reduced disease incidence.
Peso molecular

279.32

Fòrmula

C15H14O3.1/2Ca.H2O
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

CC(C([O-])=O)C1=CC(OC2=CC=CC=C2)=CC=C1.O.[0.5Ca2+]
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad
In Vitro: 

DMSO : ≥ 100 mg/mL (358.01 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (3.58 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5801 mL 17.9006 mL 35.8012 mL
5 mM 0.7160 mL 3.5801 mL 7.1602 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.5801 mL 17.9006 mL 35.8012 mL 89.5031 mL
DMSO 5 mM 0.7160 mL 3.5801 mL 7.1602 mL 17.9006 mL
10 mM 0.3580 mL 1.7901 mL 3.5801 mL 8.9503 mL
15 mM 0.2387 mL 1.1934 mL 2.3867 mL 5.9669 mL
20 mM 0.1790 mL 0.8950 mL 1.7901 mL 4.4752 mL
25 mM 0.1432 mL 0.7160 mL 1.4320 mL 3.5801 mL
30 mM 0.1193 mL 0.5967 mL 1.1934 mL 2.9834 mL
40 mM 0.0895 mL 0.4475 mL 0.8950 mL 2.2376 mL
50 mM 0.0716 mL 0.3580 mL 0.7160 mL 1.7901 mL
60 mM 0.0597 mL 0.2983 mL 0.5967 mL 1.4917 mL
80 mM 0.0448 mL 0.2238 mL 0.4475 mL 1.1188 mL
100 mM 0.0358 mL 0.1790 mL 0.3580 mL 0.8950 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Fenoprofen Calcium hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fenoprofen Calcium71720-56-4LILLY-53858 CalciumCOXMelanocortin ReceptorERKCyclooxygenaseMC ReceptorExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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Nombre del producto:
Fenoprofen Calcium hydrate
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