ヘスペリジン (Synonyms: Hesperidin; Hesperetin 7-rutinoside)

Name: Hesperidin
Brand: MedChemExpress
SKU: HY-15337
Rating: 5.0 (30 reviews)

製品番号: HY-15337 純度: 99.14%: Data Sheet SDS COA 取扱説明書
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Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 520-26-3

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 56	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 56	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$42	在庫あり	Estimated Time of Arrival: December 31
10 mg	$66	在庫あり	Estimated Time of Arrival: December 31
25 mg	$95	在庫あり	Estimated Time of Arrival: December 31
50 mg	$119	在庫あり	Estimated Time of Arrival: December 31
100 mg	$158	在庫あり	Estimated Time of Arrival: December 31
200 mg	$211	在庫あり	Estimated Time of Arrival: December 31
500 mg	$251	在庫あり	Estimated Time of Arrival: December 31
1 g	$383	在庫あり	Estimated Time of Arrival: December 31
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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 30 publication(s) in Google Scholar

Other Forms of Hesperidin:

Hesperidin (Standard) 在庫あり

顧客検証

In Vivo Efficacy Study

Histological Imaging/Staining

RT-PCR

: Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]; Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Average daily food intake (n = 6/group). Fecal TAG levels (n = 6/group). Liver organ index (n = 6/group). Energy expenditure (n = 6/group). Fasting blood glucose levels, fasting insulin levels, and HOMA–IR scores (n = 6/group).

: Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]; Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Western blot analysis of AKT phosphorylation in mouse livers (n = 2/group).

: Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]; Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. H & E staining of mouse liver sections (n = 6/group). Oil Red O depicting hepatic lipid droplets; scale bar, 50 μm.

: Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]; Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. RT–qPCR analysis of the mRNA levels of genes involved in hepatic inflammation (n = 6/group).

: Hesperidin purchased from MedChemExpress. Usage Cited in: Metabolism. 2025 Apr 23:169:156271. [Abstract]; Eight–week–old NTG and OE–WWP1 C57 BL/6 J mice were intragastrically administered with the novel WWP1 inhibitor hesperidin (150 mg/kg body weight/day) and further fed an ND or HFHC for another 16 weeks. Western blot detecting expressions of NF–κB pathways in livers (n = 2/group).

: Hesperidin purchased from MedChemExpress. Usage Cited in: Cell Rep. 2017 Nov 21;21(8):2147-2159. [Abstract]; U2OS cells expressing Myc-tagged PLK1 WT, K492R, or SUMO-1-K492R are treated with NSC 125973 for 16 hr. Half of the cells are then released in the presence of Hesperidin for 45 min to enrich cells at the later stage of M phase. Cell lysates are subject to IP, followed by western blot.

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	311 3 Compound: 11	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
A549	IC₅₀	> 100 3 Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
DLD-1	IC₅₀	> 100 3 Compound: 12	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
A549	IC₅₀	>100 3 Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
A549	IC₅₀	> 100 3 Compound: 12	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
H9	EC₅₀	> 164 3 Compound: 21	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
DLD-1	IC₅₀	>100 3 Compound: 12	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
H9	IC₅₀	> 164 3 Compound: 21	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
H9	EC₅₀	>164 3 Compound: 21	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
HT-29	IC₅₀	142 3 Compound: 11	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
H9	IC₅₀	>164 3 Compound: 21	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
Monocyte	IC₅₀	> 200 3 Compound: Hesperidin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]
HT-29	IC₅₀	142 3 Compound: 11	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
Monocyte	IC₅₀	>200 3 Compound: Hesperidin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]
DLD-1	IC₅₀	> 100 3 Compound: 12	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction assay	[PMID: 20413315]
H9	EC₅₀	> 164 3 Compound: 21	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	> 164 3 Compound: 21	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
HT-29	IC₅₀	142 3 Compound: 11	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
Monocyte	IC₅₀	> 200 3 Compound: Hesperidin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

体外実験

Hesperidin (5-200 μM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells^[1].
Hesperidin (5-150 μM; 48 h) induces early and late apoptosis in MG-63 cells^[1].
Hesperidin (10-30 μM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS^[2].
Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MG-63 cells
Concentration:	0, 5, 25, 50, 100, 150, 200 µM
Incubation Time:	24, 48, 72 hours
Result:	Led to time-dependent and concentration-dependent cytotoxic effects, with IC₅₀s of 94.3, 78.6 and 63.3 µM at 24, 48 and 72 h, respectively.

Apoptosis Analysis^[1]

Cell Line:	MG-63 cells
Concentration:	0, 5, 50, 150 µM
Incubation Time:	48 hours
Result:	Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 µM, respectively.

体内実験

Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice^[1].
Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

610.56

分子式

C₂₈H₃₄O₁₅

CAS 番号

520-26-3

Appearance

Solid

Color

Light yellow to khaki

SMILES

OC1=C(OC)C=CC([C@@H]2CC(C3=C(C=C(O[C@@H]4O[C@H](CO[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)C=C3O)O2)=O)=C1

Structure Classification

Initial Source

Endogenous metabolite

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (163.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6378 mL	8.1892 mL	16.3784 mL
5 mM	0.3276 mL	1.6378 mL	3.2757 mL
10 mM	0.1638 mL	0.8189 mL	1.6378 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.14%

Select Batch:

データシート (281 KB) SDS (393 KB)

COA (253 KB) HNMR (221 KB) RP-HPLC (206 KB) MS (70 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Du GY, et, al. Hesperidin exhibits in vitro and in vivo antitumor effects in human osteosarcoma MG-63 cells and xenograft mice models via inhibition of cell migration and invasion, cell cycle arrest and induction of mitochondrial-mediated apoptosis. Oncol Lett. 2018 Nov;16(5):6299-6306. [Content Brief]

[2]. Tejada S, et, al. Potential Anti-inflammatory Effects of Hesperidin from the Genus Citrus. Curr Med Chem. 2018;25(37):4929-4945. [Content Brief]

[3]. Gao G, et, al. Effects of Hesperidin on H₂O₂-Treated Chondrocytes and Cartilage in a Rat Osteoarthritis Model. Med Sci Monit. 2018 Dec 17;24:9177-9186. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6378 mL	8.1892 mL	16.3784 mL	40.9460 mL
	5 mM	0.3276 mL	1.6378 mL	3.2757 mL	8.1892 mL
	10 mM	0.1638 mL	0.8189 mL	1.6378 mL	4.0946 mL
	15 mM	0.1092 mL	0.5459 mL	1.0919 mL	2.7297 mL
	20 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	25 mM	0.0655 mL	0.3276 mL	0.6551 mL	1.6378 mL
	30 mM	0.0546 mL	0.2730 mL	0.5459 mL	1.3649 mL
	40 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	50 mM	0.0328 mL	0.1638 mL	0.3276 mL	0.8189 mL
	60 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6824 mL
	80 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL
	100 mM	0.0164 mL	0.0819 mL	0.1638 mL	0.4095 mL

Hesperidin Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hesperidin520-26-3Hesperetin 7-rutinosideApoptosisAutophagyReactive Oxygen Species (ROS)Endogenous MetaboliteflavanoneglycosidecitrusinflammatoryantioxidantantiallergicantitumorInhibitorinhibitorinhibit

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備考

ヘスペリジン (Synonyms: Hesperidin; Hesperetin 7-rutinoside)

カスタマーレビュー

Other Forms of Hesperidin:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hesperidin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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ヘスペリジン (Synonyms: Hesperidin; Hesperetin 7-rutinoside)

カスタマーレビュー

Other Forms of Hesperidin:

ヘスペリジン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

ヘスペリジン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hesperidin Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report

ヘスペリジン関連抗体

ヘスペリジン関連抗体