1. Leonurine hydrochloride

Leonurine hydrochloride  (Synonyms: SCM-198 hydrochloride)

Cat. No.: HY-N0741A Purity: 99.69%
COA Handling Instructions

Leonurine hydrochloride is an alkaloid isolated from Leonurus artemisia, with anti-oxidative and anti-inflammatory.

For research use only. We do not sell to patients.

Leonurine hydrochloride Chemical Structure

Leonurine hydrochloride Chemical Structure

CAS No. : 24735-18-0

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
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10 mg USD 60 In-stock
25 mg USD 130 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Leonurine hydrochloride:

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  • Biological Activity

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  • Purity & Documentation

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Description

Leonurine hydrochloride is an alkaloid isolated from Leonurus artemisia, with anti-oxidative and anti-inflammatory.

In Vitro

Leonurine (0, 5, 10, 20, 40, 80 μM) causes diminution in lipid accumulation, cellular cholesterol content, including total cholesterol (TC), free cholesterol (FC) and cholesteryl ester (CE), and increase in apoA-I- or HDL-mediated cholesterol efflux after treatment for 24 h. Leonurine also significantly and dose-dependently increases the expressions of ABCA1 and ABCG1 at the mRNA and protein levels in human THP-1 macrophages, and such effect is involved in PPARγ[1]. Leonurine hydrochloride (LH) shows protective effect on cell viability of HepG2 and HL-7702 cells incubated with palmitic acid (PA) of free fatty acid (FFA) for 24 h. Leonurine hydrochloride (125, 250, 500 μM) improves cellular lipid accumulation in HepG2 and HL-7702 cells via activating AMPK/SREBP1 pathway[2]. Leonurine (5, 10, 20 μM) inhibits the expression of iNOS, COX-2, PGE2, NO, TNF-α, and IL-6 in IL-1β-induced human chondrocytes, suppresses ECM degradation in human OA chondrocytes, and blocks IL-1β-induced PI3K and Akt phosphorylation in a dose-dependent manner[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Leonurine (10 mg/kg/d, p.o.) significantly increases the expressions of PPARγ, LXRα, ABCA1 and ABCG1, and decreases both TG and TC levels in serum of mice[1]. Leonurine hydrochloride (50, 100, 200 mg/kg) improves intracellular lipid accumulation via activating AMPK/SREBP1 pathway, enhances biochemical parameters, reduces hepatic lipoperoxide and increases antioxidant levels in mice[2]. Leonurine (20 mg/kg, p.o.) ameliorates osteoarthritis development in mouse DMM model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

347.79

Formula

C14H22ClN3O5

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCCCNC(N)=N)C1=CC(OC)=C(O)C(OC)=C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL (89.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (14.38 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8753 mL 14.3765 mL 28.7530 mL
5 mM 0.5751 mL 2.8753 mL 5.7506 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
Cell Assay
[2]

MTT assay is performed to study the cytotoxic effects of Leonurinein HepG2 and HL-7702 cells. Briefly, HepG2 and HL-7702 cells are seeded for 24 h at the density of 3 × 104 cells/well in 96-well plates. After 24 h incubation, cells are treated with different concentrations of Leonurine (0-1000 μM) and the control group is treated with only DMEM for 24 h at 37°C in 5% CO2 incubator. Then, these cells are treated with MTT solution (5 mg/mL) for further 4 h. After 4 h incubation, DMEM containing MTT solution is discarded. Cells are then dissolved by adding DMSO (200 μL) to each well and the solutions are mixed thoroughly for 5 min. Finally, the absorbance is determined at 570 nm with a microplate reader[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice ApoE-/- mice (male, eight-week old) are fed a chow diet for 2 weeks, apoE-/- mice are randomly divided into several groups (n=15/group). Mice in the Leonurine group are intragastrically administered with Leonurine (10 mg/kg/d) every day and continued for 8 weeks. The control group is fed with an equal volume of PBS. At week 16, mice are euthanized, followed by collecting the blood and tissue samples for further analyses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.8753 mL 14.3765 mL 28.7530 mL 71.8825 mL
5 mM 0.5751 mL 2.8753 mL 5.7506 mL 14.3765 mL
10 mM 0.2875 mL 1.4376 mL 2.8753 mL 7.1882 mL
DMSO 15 mM 0.1917 mL 0.9584 mL 1.9169 mL 4.7922 mL
20 mM 0.1438 mL 0.7188 mL 1.4376 mL 3.5941 mL
25 mM 0.1150 mL 0.5751 mL 1.1501 mL 2.8753 mL
30 mM 0.0958 mL 0.4792 mL 0.9584 mL 2.3961 mL
40 mM 0.0719 mL 0.3594 mL 0.7188 mL 1.7971 mL
50 mM 0.0575 mL 0.2875 mL 0.5751 mL 1.4376 mL
60 mM 0.0479 mL 0.2396 mL 0.4792 mL 1.1980 mL
80 mM 0.0359 mL 0.1797 mL 0.3594 mL 0.8985 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Leonurine hydrochloride
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