2. Anti-infection Stem Cell/Wnt
  3. Bacterial Antibiotic YAP
  4. Thiostrepton

チオストレプトン  (Synonyms: Thiostrepton)

製品番号: HY-B0990 純度: 99.80%
COA 取扱説明書 Technical Support

Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. FOXM1 binds to YAP/TEAD complex. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

カスタムペプチド

CAS 番号 : 1393-48-2

容量 価格（税別） 在庫状況 数量
50 mg $50 在庫あり
100 mg $75 在庫あり
500 mg $150 在庫あり
1 g $225 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 24 publication(s) in Google Scholar

チオストレプトン 関連抗体

Top Publications Citing Use of Products

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    Thiostrepton (TST) (2-5 μM) significantly inhibited the clonogenesis of Panc-1, MIAPaCa-2 and BxPC-3 cells.

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    Thiostrepton (TST) (2-5 μM; 48 h) significantly reduced factor forkhead box M1 (FOXM1) protein expression levels in Panc-1, MIAPaCa-2 and BxPC-3 cells.

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    Thiostrepton (TST) (2 μM; 24 h) significantly increased ROS production in Panc-1, MIA PaCa-2, and BxPC-3 cells.

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    Thiostrepton (TST) (2-5 μM) reduced the expression of STAT3 both in nucleus and cytoplasm of Panc-1, MIA PaCa-2, and BxPC-3 cells.

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    Thiostrepton (TST) (17 mg/kg; i.p.; three times a week) effectively decreased the levels of FOXM1, STAT3, p-STA3, GPX4, and Ki67 proteins in the subcutaneous xenograft model mice.

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Oncogene. 2018 Oct;37(41):5520-5533.  [Abstract]

    Proliferation of CLL cells with lentivirus mediated FoxM1 knockdown or incubation of 1 μM FoxM1 inhibitor Thiostrepton treatment reduces significantly relative to the control group (p<0.05).

    Thiostrepton purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2018 Aug 17;242(Pt B):1535-1545.  [Abstract]

    RWPE-1 cells are pretreated with Thiostrepton at 5 µM for 1 h and subsequently exposed to MC-LR (100 nM) for 48 h; expressions of FOXM1, β-catenin, CyclinD1 and PSA are detected by western blotting. Expression levels of FOXM1 and β-catenin in the nuclei of RWPE-1 cells are examined by western blotting assay.

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    YAP アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Thiostrepton is a thiazole antibiotic which selectively inhibits FOXM1. FOXM1 binds to YAP/TEAD complex. YAP/TEAD/FOXM1 complex binding at regulatory regions of genes governing cell cycle may impact cell proliferation[1].

    IC50 & Target

    YAP/TAZ-TEAD

     

    体外実験

    Thiostrepton (0.01-1000 μM; 48 hours) suppresses cell viability in A2780 and HEC-1A[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    チオストレプトン 関連抗体

    Cell Viability Assay[2]

    Cell Line: A2780 and HEC-1A cells
    Concentration: 0.01, 0.1, 1, 10, 100, 1000 μM
    Incubation Time: 48 hours
    Result: The IC50s are 1.10 μM in A2780 and 2.22 μM in HEC-1A, respectively.
    体内実験

    Thiostrepton (i.p.; 17 mg/kg) reduces the tumorigenicity of Ewing's sarcoma (EWS) cells. Tumor volumes in control mice have increased ~6-fold from the initiation of treatment, while their Thiostrepton-treated counterparts increase only ~1.7-fold, exhibiting a ~3.5-fold reduction, relative to controls[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Athymic (BALB/c nu/nu) nude mice bearing A4573 cells[3]
    Dosage: 17 mg/kg
    Administration: Administered i.p.
    Result: Treatment inhibited the growth of EWS-derived tumors in vivo.
    分子量

    1664.89

    分子式

    C72H85N19O18S5
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Structure Classification
    Initial Source

    bacteria of Streptomyces genus
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Sealed storage, away from moisture
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 125 mg/mL (75.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.6006 mL 3.0032 mL 6.0064 mL
    5 mM 0.1201 mL 0.6006 mL 1.2013 mL
    10 mM 0.0601 mL 0.3003 mL 0.6006 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.80%

    COA (271 KB) LCMS (86 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.6006 mL 3.0032 mL 6.0064 mL 15.0160 mL
    5 mM 0.1201 mL 0.6006 mL 1.2013 mL 3.0032 mL
    10 mM 0.0601 mL 0.3003 mL 0.6006 mL 1.5016 mL
    15 mM 0.0400 mL 0.2002 mL 0.4004 mL 1.0011 mL
    20 mM 0.0300 mL 0.1502 mL 0.3003 mL 0.7508 mL
    25 mM 0.0240 mL 0.1201 mL 0.2403 mL 0.6006 mL
    30 mM 0.0200 mL 0.1001 mL 0.2002 mL 0.5005 mL
    40 mM 0.0150 mL 0.0751 mL 0.1502 mL 0.3754 mL
    50 mM 0.0120 mL 0.0601 mL 0.1201 mL 0.3003 mL
    60 mM 0.0100 mL 0.0501 mL 0.1001 mL 0.2503 mL
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    Thiostrepton Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Thiostrepton1393-48-2BacterialAntibioticYAPYes-associated proteinInhibitorinhibitorinhibit

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