2. GPCR/G Protein
  3. Free Fatty Acid Receptor
  4. Vincamine

Vincamine is a monoterpenoid indole alkaloid extracted from the Madagascar periwinkle. Vincamine is a peripheral vasodilator and exerts a selective vasoregulator action on the brain microcapilar circulation. Vincamine is a GPR40 agonist and acts as a β-cell protector by ameliorating β-cell dysfunction and promoting glucose-stimulated insulin secretion (GSIS). Vincamine improves glucose homeostasis in vivo, and has the potential for the type 2 diabetes mellitus (T2DM) research.

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Vincamine

Vincamine Estructura química

No. CAS : 1617-90-9

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
100 mg En stock
500 mg En stock
1 g En stock
5 g   Obtener un presupuesto  
10 g   Obtener un presupuesto  

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Revisión del cliente

Based on 1 publication(s) in Google Scholar

Other Forms of Vincamine:

Vincamine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Vincamine

Ver todos los productos específicos de isoformas Free Fatty Acid Receptor:

Ver todas las isoformas
FFAR1 FFAR2 FFAR3 FFAR4

  • Actividad biológica

  • Pureza y Documentación

  • Referencias

  • Revisión del cliente

Descripciòn

Vincamine is a monoterpenoid indole alkaloid extracted from the Madagascar periwinkle. Vincamine is a peripheral vasodilator and exerts a selective vasoregulator action on the brain microcapilar circulation[1]. Vincamine is a GPR40 agonist and acts as a β-cell protector by ameliorating β-cell dysfunction and promoting glucose-stimulated insulin secretion (GSIS). Vincamine improves glucose homeostasis in vivo, and has the potential for the type 2 diabetes mellitus (T2DM) research[2].

Cellular Effect
Cell Line Type Value Description References
B16 IC50
68.9 3
Compound: vincamine
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
[PMID: 20576577]
A549 IC50
309.7 3
Compound: 1
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 37579526]
A549 IC50
309.7 3
Compound: 1
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 37579526]
B16 IC50
68.9 3
Compound: vincamine
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
[PMID: 20576577]
INS1(832/13) EC50
11 3
Compound: Vincamine
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
[PMID: 31918073]
HEp-2 IC50
273.7 3
Compound: 1
Cytotoxicity against human HEp-2 cells assessed as reduction in cell growth
Cytotoxicity against human HEp-2 cells assessed as reduction in cell growth
[PMID: 37579526]
B16 IC50
68.9 3
Compound: vincamine
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
Antimelanogenic activity in mouse B16 cells assessed as decrease in intracellular melanin level after 4 days
[PMID: 20576577]
INS1(832/13) EC50
11 3
Compound: Vincamine
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
[PMID: 31918073]
KB IC50
232.5 3
Compound: 1
Cytotoxicity against human KB cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay
Cytotoxicity against human KB cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay
[PMID: 37579526]
HEp-2 IC50
273.7 3
Compound: 1
Cytotoxicity against human HEp-2 cells assessed as reduction in cell growth
Cytotoxicity against human HEp-2 cells assessed as reduction in cell growth
[PMID: 37579526]
INS1(832/13) EC50
11 3
Compound: Vincamine
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
Cytoprotective activity in rat INS-1 832/13 cells assessed as inhibition of STZ-induced apoptosis incubated for 24 hrs by MTT assay
[PMID: 31918073]
KB IC50
232.5 3
Compound: 1
Cytotoxicity against human KB cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay
Cytotoxicity against human KB cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay
[PMID: 37579526]
In Vitro

Vincamine (20, 40 and 80 μM; 24 hours) exerts a significant, concentration-dependent protective effect in LPS-treated human corneal epithelial cells (HCECs) cells[1].
Vincamine (20, 40 and 80 μM; 24 hours) significantly reduces ROS level in a dose-dependent manner in LPS-treated human corneal epithelial cells (HCECs)cells. Additionally,  after Vincamine administration, the levels of MDA is also significantly reduced while the levels of T-AOC, and SOD are increased in a dose-dependent manner[1].
Vincamine (20, 40 and 80 μM; 24 hours) rescues TrxR activity in a dose-dependent manner in HCECs. However, the intracellular activities of Trx, GR and GPx are neither inhibited nor activated by both LPS and Vincaminer[1].
Vincamine could activate GPR40 (EC50=6.28 µM) with DHA (GPR40 ligand) as a positive control (EC50=3.85 µM) in hGPR40-CHO cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Vincamine Related Antibodies
In Vivo

Vincamine (intraperitoneal injection; 15 and 30 mg/kg/day; 6 weeks) improves glucose tolerance in type 2 diabetic model mice. It effectively lowers the levels of fasting blood glucose and glycated hemoglobin. At the same time, it ameliorates oral glucose tolerance and elevated glucose-induced plasma insulin concentration without influence on basal insulin secretion in vivo[2].<.br>

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male and female db/db mice (BKS.Cg-Dock7m+/+Leprdb/J) and HFD/STZ-induced type 2 diabetic model mice
Dosage: 15 and 30 mg/kg/day
Administration: Intraperitoneal injection; 15 and 30 mg/kg/day; 6 weeks
Result: Enhanced glucose tolerance in HFD/STZ and db/db male mice.
Peso molecular

354.44

Fòrmula

C21H26N2O3
No. CAS
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)[C@@]1(O)C[C@@]2(CC)[C@@]3([H])C(N1C4=CC=CC=C54)=C5CCN3CCC2
Structure Classification
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvente y solubilidad
In Vitro: 

DMSO : 25 mg/mL (70.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8214 mL 14.1068 mL 28.2135 mL
5 mM 0.5643 mL 2.8214 mL 5.6427 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: 99.34%

SDS (393 KB)

COA (262 KB) LCMS (93 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8214 mL 14.1068 mL 28.2135 mL 70.5338 mL
5 mM 0.5643 mL 2.8214 mL 5.6427 mL 14.1068 mL
10 mM 0.2821 mL 1.4107 mL 2.8214 mL 7.0534 mL
15 mM 0.1881 mL 0.9405 mL 1.8809 mL 4.7023 mL
20 mM 0.1411 mL 0.7053 mL 1.4107 mL 3.5267 mL
25 mM 0.1129 mL 0.5643 mL 1.1285 mL 2.8214 mL
30 mM 0.0940 mL 0.4702 mL 0.9405 mL 2.3511 mL
40 mM 0.0705 mL 0.3527 mL 0.7053 mL 1.7633 mL
50 mM 0.0564 mL 0.2821 mL 0.5643 mL 1.4107 mL
60 mM 0.0470 mL 0.2351 mL 0.4702 mL 1.1756 mL
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Vincamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vincamine1617-90-9Free Fatty Acid ReceptorFFARGPR40 agonistβ-cell protectorglucose-stimulatedinsulin secretionGSIScardio-cerebrovascular diseasenootropicT2DMdietary supplementInhibitorinhibitorinhibit

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Nombre del producto:
Vincamine
Cat. No.:
HY-B1021
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SAFETY DATA SHEET (SDS)

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