2. Others Neuronal Signaling Membrane Transporter/Ion Channel Cell Cycle/DNA Damage Cytoskeleton Autophagy
  3. Biochemical Assay Reagents TRP Channel Microtubule/Tubulin Autophagy
  4. Estradiol 3-methyl ether

Estradiol 3-methyl ether  (Synonyms: 3-O-Methyl estradiol; 17β-Estradiol 3-methyl ether; 3-Methoxyestradiol)

Cat. No.: HY-W008581 Purity: 99.07%
COA
SDS
Handling Instructions Technical Support

Estradiol 3-methyl ether (EDME) is a highly selective TRPML1 ion channel antagonist and microtubule (microtubule) depolymerizing agent, with IC50 values of 0.22 μM and 3.8 μM against TRPML1 and TRPML2, respectively; it shows no activity against TRPML3. Estradiol 3-methyl ether induces the disruption of cytoplasmic microtubule networks in mammalian cells, with an EC50 of 9 μM. Independent of estrogen receptors, Estradiol 3-methyl ether blocks autophagy (autophagy), TFEB nuclear translocation, and inhibits the migration and invasion of triple-negative breast cancer cells by suppressing TRPML1. Estradiol 3-methyl ether is applicable for relevant research on triple-negative breast cancer.

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Estradiol 3-methyl ether

Estradiol 3-methyl ether 화학구조

CAS No. : 1035-77-4

사이즈 가격 재고 수량
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg   견적 받기  
200 mg   견적 받기  

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Estradiol 3-methyl ether Related Antibodies

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TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Estradiol 3-methyl ether (EDME) is a highly selective TRPML1 ion channel antagonist and microtubule (microtubule) depolymerizing agent, with IC50 values of 0.22 μM and 3.8 μM against TRPML1 and TRPML2, respectively; it shows no activity against TRPML3. Estradiol 3-methyl ether induces the disruption of cytoplasmic microtubule networks in mammalian cells, with an EC50 of 9 μM. Independent of estrogen receptors, Estradiol 3-methyl ether blocks autophagy (autophagy), TFEB nuclear translocation, and inhibits the migration and invasion of triple-negative breast cancer cells by suppressing TRPML1. Estradiol 3-methyl ether is applicable for relevant research on triple-negative breast cancer[1][2].

In Vitro

Estradiol 3-methyl ether (1 μM; 3 h) inhibits TFEB nuclear translocation and blocks the induction of autophagy[1].
Estradiol 3-methyl ether (0.1-10 μM) significantly inhibits the invasive ability of MDA-MB-231 WT cells in a dose-dependent manner[1].
Estradiol 3-methyl ether (9-100 μM; 1 h) induces microtubule breakage in Chinese hamster V79 cells, with an EC50 of 9 μM after 1 h of incubation, and completely abolishes the normal microtubule network at a concentration of 100 μM[2].
Estradiol 3-methyl ether (20-50 μM; 2-4 days) inhibits the growth of Chinese hamster V79 cells, and treatment with 20 μM for 2 days causes 92% growth inhibition[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Estradiol 3-methyl ether Related Antibodies

Cell Proliferation Assay[2]

Cell Line: Chinese hamster V79 cells
Concentration: 20 μM (2-day incubation); 50 μM (4-day incubation)
Incubation Time: 2 days (20 μM); 4 days (50 μM)
Result: Caused 92% inhibition of cell growth at 20 μM after 2 days of treatment.
Exhibited more potent cell growth-inhibitory activity than 17β-estradiol (HY-B0141) at 50 μM over 4 days of treatment.
분자량

286.41

화학식

C19H26O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12CC[C@]3([H])C4=CC=C(OC)C=C4CC[C@@]3([H])[C@]1([H])CC[C@]2([H])O
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 100 mg/mL (349.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4915 mL 17.4575 mL 34.9150 mL
5 mM 0.6983 mL 3.4915 mL 6.9830 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4915 mL 17.4575 mL 34.9150 mL 87.2875 mL
5 mM 0.6983 mL 3.4915 mL 6.9830 mL 17.4575 mL
10 mM 0.3491 mL 1.7457 mL 3.4915 mL 8.7287 mL
15 mM 0.2328 mL 1.1638 mL 2.3277 mL 5.8192 mL
20 mM 0.1746 mL 0.8729 mL 1.7457 mL 4.3644 mL
25 mM 0.1397 mL 0.6983 mL 1.3966 mL 3.4915 mL
30 mM 0.1164 mL 0.5819 mL 1.1638 mL 2.9096 mL
40 mM 0.0873 mL 0.4364 mL 0.8729 mL 2.1822 mL
50 mM 0.0698 mL 0.3491 mL 0.6983 mL 1.7457 mL
60 mM 0.0582 mL 0.2910 mL 0.5819 mL 1.4548 mL
80 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0911 mL
100 mM 0.0349 mL 0.1746 mL 0.3491 mL 0.8729 mL
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Estradiol 3-methyl ether Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Estradiol 3-methyl ether1035-77-43-O-Methyl estradiol 17β-Estradiol 3-methyl ether 3-MethoxyestradiolBiochemical Assay ReagentsTRP ChannelMicrotubule/TubulinAutophagyTransient receptor potential channelsTPC2triple-negative breast cancer cellsTRPML1 channelTFEBChinese hamster V79 cellsestrogen receptor alphamammalian cellsTRPML2TRPML3human ERαInhibitorinhibitorinhibit

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상품명:
Estradiol 3-methyl ether
Cat. No.:
HY-W008581
수량:
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