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  4. Vascular Inflammation Models
  5. L-Homocysteine

L-Homocysteine, an amino acid, is a homocysteine that has L configuration. Homocysteine is an essential intermediate in normal mammalian metabolism of methionine. L-Homocysteine induces upregulation of Cathepsin V that mediates vascular endothelial inflammation in hyperhomocysteinaemia.

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CAS No. : 6027-13-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Solution
10 mM * 1 mL in Water In-stock
Solid
25 mg In-stock
50 mg In-stock
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Based on 7 publication(s) in Google Scholar

Other Forms of L-Homocysteine:

L-Homocysteine Related Antibodies

Top Publications Citing Use of Products

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    CCK-8 assay evaluated the vitality of HUVECs under various concentrations of L-Homocysteine (Hcy; 2.5, 5 or 7.5 mM; 24; 48, 72 h).

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    qPCR evaluated the expression of CNTF in HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    Western blotting evaluated the expression of CNTF in HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    ELISA was used to measure the levels of CNTF in the culture medium of HUVECs treated with L-Homocysteine (Hcy; 7.5 mM; 24 h).

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    Western blotting was employed to analyze the levels of p-JAK2, JAK2, p-STAT3, STAT3, and IL-6 in HUVECs exposed to varying concentrations of L-Homocysteine (Hcy; 7.5 mM; 24 h).

    L-Homocysteine purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2024 Jun 5:114103.  [Abstract]

    Flow cytometry detected the apoptosis of HUVECs induced by L-Homocysteine (Hcy; 7.5 mM; 24 h).

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

    View All Cathepsin Isoform Specific Products:

    View All Isoforms
    Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Homocysteine, an amino acid, is a homocysteine that has L configuration. Homocysteine is an essential intermediate in normal mammalian metabolism of methionine. L-Homocysteine induces upregulation of Cathepsin V that mediates vascular endothelial inflammation in hyperhomocysteinaemia[1][2].

    IC50 & Target[1][2]

    Human Endogenous Metabolite

     

    cathepsin L

     

    In Vitro

    L-Homocysteine (0.5, 1, 2 mM; 24 h) increases the mRNA and protein levels of Cathepsin V in a dose‐dependent manner[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Homocysteine Related Antibodies

    Western Blot Analysis[2]

    Cell Line: HUVECs
    Concentration: 0.5, 1, 2 mM
    Incubation Time: 24 h
    Result: Increased the mRNA and protein levels of Cathepsin V in a dose‐dependent manner.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    L-Homocysteine can be used in animal modeling to construct a model of vascular endothelial inflammation.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    135.19

    Formula

    C4H9NO2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CCS)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (184.92 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.3970 mL 36.9850 mL 73.9700 mL
    5 mM 1.4794 mL 7.3970 mL 14.7940 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (369.85 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (244 KB) HNMR (237 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 7.3970 mL 36.9850 mL 73.9700 mL 184.9249 mL
    5 mM 1.4794 mL 7.3970 mL 14.7940 mL 36.9850 mL
    10 mM 0.7397 mL 3.6985 mL 7.3970 mL 18.4925 mL
    15 mM 0.4931 mL 2.4657 mL 4.9313 mL 12.3283 mL
    20 mM 0.3698 mL 1.8492 mL 3.6985 mL 9.2462 mL
    25 mM 0.2959 mL 1.4794 mL 2.9588 mL 7.3970 mL
    30 mM 0.2466 mL 1.2328 mL 2.4657 mL 6.1642 mL
    40 mM 0.1849 mL 0.9246 mL 1.8492 mL 4.6231 mL
    50 mM 0.1479 mL 0.7397 mL 1.4794 mL 3.6985 mL
    60 mM 0.1233 mL 0.6164 mL 1.2328 mL 3.0821 mL
    80 mM 0.0925 mL 0.4623 mL 0.9246 mL 2.3116 mL
    100 mM 0.0740 mL 0.3698 mL 0.7397 mL 1.8492 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Homocysteine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Homocysteine6027-13-0Endogenous MetaboliteCathepsinhomocysteineSAHendogenousmetaboliteInhibitorinhibitorinhibit

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