Lonidamine (Synonyms: DICA; AF-1890; ロニダミン; Diclondazolic Acid)

Name: Lonidamine
Brand: MedChemExpress
SKU: HY-B0486
Rating: 5.0 (17 reviews)

製品番号: HY-B0486 純度: 99.91%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Lonidamine (AF-1890) is a hexokinase and mitochondrial pyruvate carrier inhibitor (K_i: 2.5 μM). Lonidamine also inhibits aerobic glycolysis in cancer cells. Lonidamine can be used in the research of mitochondrial metabolism and inflammation, such as pulmonary fibrosis.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 50264-69-2

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 72	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$66	在庫あり	Estimated Time of Arrival: December 31
10 mg	$79	在庫あり	Estimated Time of Arrival: December 31
25 mg	$118	在庫あり	Estimated Time of Arrival: December 31
50 mg	$158	在庫あり	Estimated Time of Arrival: December 31
100 mg	$190	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of Lonidamine:

Lonidamine (Standard) 在庫あり

顧客検証

IHC

RT-PCR

: Lonidamine purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Sep 26;10(1):310. [Abstract]; Lonidamine (1 μM; 3-24 h) treatment led to an initial reduction of the HK2 level in TNBC cells followed by eventual regeneration.

: Lonidamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Sep 6;34(9):1312-1324.e6. [Abstract]; Lonidamine (100 mg/kg; i.p.; every two days) treatment reduced HK2-mediated IkBα T291 phosphorylation and PD-L1 expression, accompanied by increased CD8⁺ T cell tumor infiltration and enhanced granzyme B expression in C57BL/6 mice.

: Lonidamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Sep 6;34(9):1312-1324.e6. [Abstract]; Lonidamine (200 μM; 12 h) partially blocked the increase in PD-L1 expression induced by expression of activated EGFR variant III (EGFRvIII) mutant in LN229 GBM cells.

: Lonidamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):1999-2017.e10. [Abstract]; Combination treatment with Rimegepant and Lonidamine, Anlotinib, or Bevacizumab led to a reduced proportion of Ki67+ cancer cells, reduce LC3B puncta, and the accumulation of p62 puncta in cancer cells, indicating impaired cell proliferation and autophagic flux.

: Lonidamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):1999-2017.e10. [Abstract]; Lonidamine (100 μM; 24 h) in vitro significantly increased NGF and Calca levels in Cal27 cells.

: Lonidamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):1999-2017.e10. [Abstract]; Lonidamine (100 μM; 24 h) activated the JNK-c-Jun pathway in Cal27 cells.

Parasite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

Ki: 2.5 μM (Mitochondrial pyruvate carrier)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	152.9 3 Compound: LND	Antiproliferative activity against mouse 4T1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against mouse 4T1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
786-0	IC₅₀	120.43 3 Compound: LND	Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
HGC-27	IC₅₀	77.62 3 Compound: LND	Antiproliferative activity against human HGC-27 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
MCF7	IC₅₀	197.45 3 Compound: LND	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
PANC-1	IC₅₀	164.51 3 Compound: LND	Antiproliferative activity against human PANC-1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human PANC-1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
4T1	IC₅₀	152.9 3 Compound: LND	Antiproliferative activity against mouse 4T1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against mouse 4T1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
786-0	IC₅₀	120.43 3 Compound: LND	Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human 786-0 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
HGC-27	IC₅₀	77.62 3 Compound: LND	Antiproliferative activity against human HGC-27 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
MCF7	IC₅₀	197.45 3 Compound: LND	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]
PANC-1	IC₅₀	164.51 3 Compound: LND	Antiproliferative activity against human PANC-1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay Antiproliferative activity against human PANC-1 cells assessed as cell growth inhibition measured for 72 hrs by MTT assay	[PMID: 37376788]

体外実験

Lonidamine (100 μM, 24 h) inhibits TGF-β-stimulated lactate production and oxygen consumption rate in AKR-2B and TIG-1 cells^[3].
Lonidamine (100 μM, 24/48 h) inhibits H2030BrM3 and A549 cell proliferation^[4].
Lonidamine (100-200 μM, 24 h) inhibits H2030BrM3 and A549 cell invasion^[4].
Lonidamine (100-1000 μM, 24 h) inhibits mitochondrial complex I and II activities^[4].
Lonidamine (200 μM, 24 h) increases ROS generation in H2030BrM3 lung cancer cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Lonidamine (oral administration, 10-100 mg/kg/day, d10 to d20) improves lung function by inhibiting hexokinase 2 (HK2) activity in BLM-induced pulmonary fibrosis murine model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Lonidamine (oral administration, 10-100 mg/kg/day, d10 to d20) improves lung function by inhibiting hexokinase 2 (HK2) activity in BLM-induced pulmonary fibrosis murine model^[3].
Dosage:	10, 30, 100 mg/kg/day
Administration:	Oral administration, daily, d10 to d20 after BLM treatment.
Result:	Partially or completely reversed the increases in HK2 and lactate induced by BLM and reduced the expression of 10 profibrotic mediators.

臨床実験

分子量

321.16

分子式

C₁₅H₁₀Cl₂N₂O₂

CAS 番号

50264-69-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NN(CC2=CC=C(Cl)C=C2Cl)C3=C1C=CC=C3)O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (155.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1137 mL	15.5686 mL	31.1371 mL
5 mM	0.6227 mL	3.1137 mL	6.2274 mL
10 mM	0.3114 mL	1.5569 mL	3.1137 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.91%

Select Batch:

データシート (281 KB) SDS (393 KB)

COA (256 KB) HNMR (282 KB) RP-HPLC (244 KB) MS (71 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Nancolas B, et al. The anti-tumour agent lonidamine is a potent inhibitor of the mitochondrial pyruvate carrier and plasma membrane monocarboxylate transporters. Biochem J. 2016 Apr 1;473(7):929-36. [Content Brief]

[2]. Ilya A Shutkov, et al. Ru(III) Complexes with Lonidamine-Modified Ligands. Int J Mol Sci. 2021 Dec 15;22(24):13468. [Content Brief]

[3]. Xueqian Yin, et al. Hexokinase 2 couples glycolysis with the profibrotic actions of TGF-β. Sci Signal. 2019 Dec 17;12(612):eaax4067. [Content Brief]

[4]. Gang Cheng, et al. Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis. Nat Commun. 2019 May 17;10(1):2205. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1137 mL	15.5686 mL	31.1371 mL	77.8428 mL
	5 mM	0.6227 mL	3.1137 mL	6.2274 mL	15.5686 mL
	10 mM	0.3114 mL	1.5569 mL	3.1137 mL	7.7843 mL
	15 mM	0.2076 mL	1.0379 mL	2.0758 mL	5.1895 mL
	20 mM	0.1557 mL	0.7784 mL	1.5569 mL	3.8921 mL
	25 mM	0.1245 mL	0.6227 mL	1.2455 mL	3.1137 mL
	30 mM	0.1038 mL	0.5190 mL	1.0379 mL	2.5948 mL
	40 mM	0.0778 mL	0.3892 mL	0.7784 mL	1.9461 mL
	50 mM	0.0623 mL	0.3114 mL	0.6227 mL	1.5569 mL
	60 mM	0.0519 mL	0.2595 mL	0.5190 mL	1.2974 mL
	80 mM	0.0389 mL	0.1946 mL	0.3892 mL	0.9730 mL
	100 mM	0.0311 mL	0.1557 mL	0.3114 mL	0.7784 mL

Lonidamine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

最近チェックした製品:

製品名

カスタマ需要量 *

お名前 *

タイトル

メールアドレス *

電話番号 *

デパートメント

組纖名 *

市区町村

都道府県

国或いは地域 *

必ず会社名を記載ください。個人への返信は行いません。

備考

製品名

Lot #

お名前 *

メールアドレス *

電話番号 *

部門 *

組纖名 *

国或いは地域 *

備考

Lonidamine (Synonyms: DICA; AF-1890; ロニダミン; Diclondazolic Acid)

カスタマーレビュー

Other Forms of Lonidamine:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Parasite アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Lonidamine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

Lonidamine (Synonyms: DICA; AF-1890; ロニダミン; Diclondazolic Acid)

カスタマーレビュー

Other Forms of Lonidamine:

Lonidamine 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Parasite アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Lonidamine 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Lonidamine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report