2. Anti-infection Metabolic Enzyme/Protease Apoptosis
  3. Bacterial MMP Apoptosis Fungal
  4. Methylisothiazolinone hydrochloride

2-メチル-4-イソチアゾリン-3-オン 塩酸塩  (Synonyms: Methylisothiazolinone hydrochloride)

製品番号: HY-W010243 純度: 99.93%
COA 取扱説明書 Technical Support

Methylisothiazolinone hydrochloride is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone hydrochloride can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone hydrochloride can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone hydrochloride can cause mitochondrial damage in the endothelium of rat cerebral blood vessels.

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Methylisothiazolinone hydrochloride

2-メチル-4-イソチアゾリン-3-オン 塩酸塩 構造式

CAS 番号 : 26172-54-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 38 在庫あり
Solution
10 mM * 1 mL in DMSO USD 38 在庫あり
Solid
1 g $35 在庫あり
5 g $70 在庫あり
25 g $140 在庫あり
100 g $260 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 0 publication(s) in Google Scholar

Other Forms of Methylisothiazolinone hydrochloride:

2-メチル-4-イソチアゾリン-3-オン 塩酸塩 関連抗体

Top Publications Citing Use of Products

MMP アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

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  • 純度とドキュメンテーション

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製品説明

Methylisothiazolinone hydrochloride is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone hydrochloride can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone hydrochloride can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone hydrochloride can cause mitochondrial damage in the endothelium of rat cerebral blood vessels[1][2][3][4].
体外実験

Methylisothiazolinone (0-8 μg/mL, 24 h) hydrochloride induces cell apoptosis and inflammatory response by activating matrix metalloproteinases (MMPs) in human bronchial epithelial cells[1].
Methylisothiazolinone (0-2.5 μg/mL, 1 h) hydrochloride can cause mitochondrial damage in brain endothelial cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-メチル-4-イソチアゾリン-3-オン 塩酸塩 関連抗体

Cell Viability Assay[1]

Cell Line: Bronchial epithelial cells (BEAS-2B cells)
Concentration: 0, 2, 4, 8 μg/mL
Incubation Time: 24 h
Result: Caused a decrease in cell viability to 81.0%, 67.9%, and 35.1% at doses of 2, 4, and 8 μg/mL, respectively.

Western Blot Analysis[1]

Cell Line: bEND.3 cell
Concentration: 0, 1, 2.5 μg/mL CMIT/MIT
Incubation Time: 1 h
Result: Increased the level of Drp-1 phosphorylated protein and decreased the level of Mfn-1 phosphorylated protein.
体内実験

Methylisothiazolinone (0.15 mg/kg; once; i.v.) hydrochloride can increase the susceptibility of rat cerebral vascular endothelium to endogenous pathological stimuli and lead to mitochondrial damage[2].
Methylisothiazolinone (20 or 40 μL; handling skin (20 μL), days 1 and 8; intranasal instillation (40 μL), day 15) hydrochloride can cause sensitization in asthmatic mice when handled on the skin[3].
Methylisothiazolinone (0.1875 mg/kg CMIT/MIT; once daily; 15 days; epidermal contact) hydrochloride promotes the development of atopic dermatitis in mice through Th2/Th17 related immune response dysregulation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male SD rats (230-300 g and 7-8 weeks of age)[2]
Dosage: 0.15 mg/kg
Administration: Intravenous injection (i.v.); once
Result: Resulted in mitochondrial damage and a decrease in tight junction proteins.
Animal Model: Male C57Bl/6 and BALB/c mouse asthma models induced by chemistry (approximately 20 g, 6 weeks old)[3]
Dosage: 20 or 40 μL
Administration: Handled skin (20 μL), on days 1 and 8; intranasal instillation (40 μL), on day 15
Result: Induced an increase in T and B cell proliferation in ear lymph nodes, while producing IFN-γ.
Animal Model: Specific dermatitis (AD) female BALB/c mice established by sensitization of ovalbumin (OVA) epidermis (5 weeks old, n = 5 per group) weighing 16-20 g[4]
Dosage: 0.1875 mg/kg chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT)
Administration: Epidermal contact; once daily; 15 days
Result: Augmented the mRNA expression level of T helper (Th) 2-related cytokines (thymic stromal lymphopoietin, interleukin (IL)-6 and IL-13), Th2 chemokine (chemokine (C-C motif) ligand 17) and the population of CD4IL-4 cells in the skin.
分子量

151.61

分子式

C4H6ClNOS
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C)SC=C1.[H]Cl
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (659.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5959 mL 32.9794 mL 65.9587 mL
5 mM 1.3192 mL 6.5959 mL 13.1917 mL
10 mM 0.6596 mL 3.2979 mL 6.5959 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.93%

COA (255 KB) HNMR (117 KB) RP-HPLC (223 KB) LCMS (193 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.5959 mL 32.9794 mL 65.9587 mL 164.8968 mL
5 mM 1.3192 mL 6.5959 mL 13.1917 mL 32.9794 mL
10 mM 0.6596 mL 3.2979 mL 6.5959 mL 16.4897 mL
15 mM 0.4397 mL 2.1986 mL 4.3972 mL 10.9931 mL
20 mM 0.3298 mL 1.6490 mL 3.2979 mL 8.2448 mL
25 mM 0.2638 mL 1.3192 mL 2.6383 mL 6.5959 mL
30 mM 0.2199 mL 1.0993 mL 2.1986 mL 5.4966 mL
40 mM 0.1649 mL 0.8245 mL 1.6490 mL 4.1224 mL
50 mM 0.1319 mL 0.6596 mL 1.3192 mL 3.2979 mL
60 mM 0.1099 mL 0.5497 mL 1.0993 mL 2.7483 mL
80 mM 0.0824 mL 0.4122 mL 0.8245 mL 2.0612 mL
100 mM 0.0660 mL 0.3298 mL 0.6596 mL 1.6490 mL
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Methylisothiazolinone hydrochloride Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylisothiazolinone26172-54-3BacterialMMPApoptosisFungalMatrix metalloproteinasessterilizationallergymitochondrial damagedermatitisInhibitorinhibitorinhibit

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製品名:
Methylisothiazolinone hydrochloride
製品番号:
HY-W010243
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