2. Neuronal Signaling Metabolic Enzyme/Protease
  3. Monoamine Oxidase Endogenous Metabolite
  4. Norharmane

ノルハルマン  (Synonyms: Norharmane; Norharman; β-Carboline)

製品番号: HY-W008566 純度: 99.93%
COA 取扱説明書 Technical Support

Norharmane (Norharman), a β-carboline alkaloid, is a potent and reversible monoamine oxidase inhibitor, with IC50 values of 6.5 and 4.7 μM for MAO-A and MAO-B, respectively. Norharmane causes antidepressant responses. Norharmane is also a prospective anti-cancer photosensitizer. Norharmane alters polar auxin transport (PAT) by inhibiting PIN2, PIN3 and PIN7 transport proteins, thus causing a significant inhibitory effect on the growth of Arabidopsis thaliana seedlings.

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Norharmane

ノルハルマン 構造式

CAS 番号 : 244-63-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
100 mg $30 在庫あり
500 mg $90 在庫あり
1 g $130 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of Norharmane:

ノルハルマン 関連抗体

Top Publications Citing Use of Products

Monoamine Oxidase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
MAO-A MAO-B

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Human Endogenous Metabolite Microbial Metabolite

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製品説明

Norharmane (Norharman), a β-carboline alkaloid, is a potent and reversible monoamine oxidase inhibitor, with IC50 values of 6.5 and 4.7 μM for MAO-A and MAO-B, respectively. Norharmane causes antidepressant responses. Norharmane is also a prospective anti-cancer photosensitizer. Norharmane alters polar auxin transport (PAT) by inhibiting PIN2, PIN3 and PIN7 transport proteins, thus causing a significant inhibitory effect on the growth of Arabidopsis thaliana seedlings[1][2][3][4][5][6].

IC50 & Target[1]

MAO-B

4.7 μM (IC50)

Microbial Metabolite

 

MAO-A

6.5 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
A549 IC50
> 100 3
Compound: 4
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
A549 IC50
> 100 3
Compound: 4
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
A549 IC50
>100 3
Compound: 4
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
Antiproliferative activity against human A549 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
HeLa IC50
> 100 3
Compound: 4
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
10.1039/C5MD00581G
H9 EC50
44.1 3
Compound: 7
Antiviral activity against HIV1 in human H9 cells assessed as inhibition of viral replication
Antiviral activity against HIV1 in human H9 cells assessed as inhibition of viral replication
[PMID: 11473435]
HeLa S3 IC50
> 100 3
Compound: norharman
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
[PMID: 21194810]
H9 IC50
141.7 3
Compound: 7
Cytotoxicity against human H9 cells
Cytotoxicity against human H9 cells
[PMID: 11473435]
HeLa IC50
>100 3
Compound: 4
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
10.1039/C5MD00581G
HL-60 IC50
> 100 3
Compound: 4
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
MCF7 IC50
> 100 3
Compound: 4
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
H9 EC50
44.1 3
Compound: 7
Antiviral activity against HIV1 in human H9 cells assessed as inhibition of viral replication
Antiviral activity against HIV1 in human H9 cells assessed as inhibition of viral replication
[PMID: 11473435]
HeLa IC50
15 3
Compound: 2
Inhibitory activity against IkappaB kinase(IKK) isolated from HeLa cells activated with recombinant MEEK1 in an ELISA phosphorylation assay.
Inhibitory activity against IkappaB kinase(IKK) isolated from HeLa cells activated with recombinant MEEK1 in an ELISA phosphorylation assay.
[PMID: 12824047]
SGC-7901 IC50
> 100 3
Compound: 4
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
HeLa S3 IC50
>100 3
Compound: norharman
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
[PMID: 21194810]
H9 IC50
141.7 3
Compound: 7
Cytotoxicity against human H9 cells
Cytotoxicity against human H9 cells
[PMID: 11473435]
HL-60 IC50
>100 3
Compound: 4
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
MCF7 IC50
>100 3
Compound: 4
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
HL-60 IC50
> 100 3
Compound: 4
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
P388 IC50
25000 14
Compound: 10
Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
[PMID: 1791472]
HeLa IC50
15 3
Compound: 2
Inhibitory activity against IkappaB kinase(IKK) isolated from HeLa cells activated with recombinant MEEK1 in an ELISA phosphorylation assay.
Inhibitory activity against IkappaB kinase(IKK) isolated from HeLa cells activated with recombinant MEEK1 in an ELISA phosphorylation assay.
[PMID: 12824047]
Platelet IC50
130 3
Compound: Norharmane
Antiplatelet activity in rabbit platelets assessed as inhibition of collagen-induced platelet aggregation
Antiplatelet activity in rabbit platelets assessed as inhibition of collagen-induced platelet aggregation
[PMID: 21983333]
HeLa IC50
> 100 3
Compound: 4
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay
10.1039/C5MD00581G
Platelet IC50
>130 3
Compound: Norharmane
Antiplatelet aggregation activity against collagen-induced platelet
Antiplatelet aggregation activity against collagen-induced platelet
[PMID: 20329729]
HeLa S3 IC50
> 100 3
Compound: norharman
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
Antitumor activity against human HeLaS3 cells after 24 to 72 hrs by MTT assay
[PMID: 21194810]
SGC-7901 IC50
>100 3
Compound: 4
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
MCF7 IC50
> 100 3
Compound: 4
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
P388 IC50
25000 14
Compound: 10
Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
[PMID: 1791472]
Platelet IC50
130 3
Compound: Norharmane
Antiplatelet activity in rabbit platelets assessed as inhibition of collagen-induced platelet aggregation
Antiplatelet activity in rabbit platelets assessed as inhibition of collagen-induced platelet aggregation
[PMID: 21983333]
Platelet IC50
> 130 3
Compound: Norharmane
Antiplatelet aggregation activity against collagen-induced platelet
Antiplatelet aggregation activity against collagen-induced platelet
[PMID: 20329729]
SGC-7901 IC50
> 100 3
Compound: 4
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 24 hrs by MTT assay
10.1039/C5MD00581G
体外実験

Norharmane attenuates quinolinic acid formation by interferon-gamma-stimulated monocytes THP-1 cells and attenuates L-kynurenine formation, with IC50 values of 51 μM and 43 μM, respectively[4].
? Norharmane has the potential for use as an antibiotic adjuvant to enhance the efficacy of conventional antibiotics to reduce pathogenic bacterial infections[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ノルハルマン 関連抗体
体内実験

Norharmane (0-10 mg/kg, IP, once) shows an anxiety- and antidepressant-like behavior, and decreased locomotor activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice (22-25 g)[3]
Dosage: 0, 2.5, 5 and 10 mg/kg
Administration: IP, once
Result: Elicited an anxiety- and antidepressant-like behavior, and decreased locomotor activity.
分子量

168.20

分子式

C11H8N2
CAS 番号
Appearance

Solid

Color

Off-white to yellow

SMILES

N1C2=C(C=CC=C2)C2=C1C=NC=C2
Structure Classification
Initial Source

Cyanobacteria
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 11 mg/mL (65.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9453 mL 29.7265 mL 59.4530 mL
5 mM 1.1891 mL 5.9453 mL 11.8906 mL
10 mM 0.5945 mL 2.9727 mL 5.9453 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.1 mg/mL (6.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.93%

COA (253 KB) HNMR (132 KB) RP-HPLC (244 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.9453 mL 29.7265 mL 59.4530 mL 148.6326 mL
5 mM 1.1891 mL 5.9453 mL 11.8906 mL 29.7265 mL
10 mM 0.5945 mL 2.9727 mL 5.9453 mL 14.8633 mL
15 mM 0.3964 mL 1.9818 mL 3.9635 mL 9.9088 mL
20 mM 0.2973 mL 1.4863 mL 2.9727 mL 7.4316 mL
25 mM 0.2378 mL 1.1891 mL 2.3781 mL 5.9453 mL
30 mM 0.1982 mL 0.9909 mL 1.9818 mL 4.9544 mL
40 mM 0.1486 mL 0.7432 mL 1.4863 mL 3.7158 mL
50 mM 0.1189 mL 0.5945 mL 1.1891 mL 2.9727 mL
60 mM 0.0991 mL 0.4954 mL 0.9909 mL 2.4772 mL
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Norharmane Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Norharmane244-63-3Norharman β-CarbolineMonoamine OxidaseEndogenous MetaboliteMAOMonoamineoxidaseCoffeeAlkaloidParkinsondiseaseNeuroprotectionInhibitorinhibitorinhibit

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製品名:
Norharmane
製品番号:
HY-W008566
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