WAY-100635

Cat. No.: HY-10349 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

WAY-100635 is a potent and selective 5-HT_1A Receptor antagonist with a pIC₅₀ of 8.87, an apparent pA₂ of 9.71. WAY-100635 is a potent and selective 5-hydroxytryptamine 1A (5-HT1A) receptor antagonist with an IC₅₀ value of 0.91 nM and K_i value of 0.39 nM. WAY-100635 has pIC₅₀ values for 5-HT1A and α1-adrenergic receptors of 8.9 and 6.6, respectively. WAY-100635 is also a potent dopamine D₄ receptor agonist.

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CAS No. : 162760-96-5

사이즈	재고	수량
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid or liquid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 12 publication(s) in Google Scholar

Other Forms of WAY-100635:

WAY-100635 maleate In-stock
WAY-100635 (Standard) 견적 받기

12 Publications Citing Use of MCE WAY-100635

In Vivo Efficacy Study

RT-PCR

: WAY-100635 purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 25;28(12):114211. [Abstract]; Experimental design for in vivo functional validation of 5-HT1A. 5-HT1A activity was inhibited by injection of 5-HT1A antagonist, WAY-100635.

: WAY-100635 purchased from MedChemExpress. Usage Cited in: Biomedicines. 2025 Sep 23;13(10):2319.; Effects of 5-HTP (10 mg/kg) and WAY100635 (1 mg/kg) on cognitive behavior and motor functions in healthy mice. The BM performance (learning and probe trials) across groups.

: WAY-100635 purchased from MedChemExpress. Usage Cited in: Biomedicines. 2025 Sep 23;13(10):2319.; Effects of 5-HTP (10 mg/kg) and WAY100635 (1 mg/kg) on cognitive behavior and motor functions in healthy mice. NORT discrimination index across group.

: WAY-100635 purchased from MedChemExpress. Usage Cited in: Biomedicines. 2025 Sep 23;13(10):2319.; Impact of WAY100635 pretreatment on 5-HTP's effects in LPS-induced SAE model. Performance during learning phase of BM between groups.

: WAY-100635 purchased from MedChemExpress. Usage Cited in: Chin Med. 2024 Jun 15;19(1):87. [Abstract]; 5-HT promoted the inflammation in H9 cells. NC, negative control; 5-HT: 5-HT (10 μM) treatment; Way: 5-HT (10 μM) and Way100635 (10 μM) treatment; Tro: 5-HT (10 μM) and Tropisetron (25 μM) treatment; Way + Tro: 5-HT (10 μM), Way100635 (10 μM) and Tropisetron (25 μM) treatment.

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View All Isoforms

5-HT Receptor 5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor

View All Dopamine Receptor Isoform Specific Products:

View All Isoforms

Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

D₄ Receptor

5-HT_1A Receptor

8.87 (pIC₅₀)

5-HT_1A Receptor

9.71 (pA2)

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	7.1 nM Compound: 1	Effective concentration against binding of radioligand [35S]GTP-gamma-S in CHO cells expressing human 5-hydroxytryptamine 1A receptor Effective concentration against binding of radioligand [35S]GTP-gamma-S in CHO cells expressing human 5-hydroxytryptamine 1A receptor	[PMID: 15239661]
CHO	EC₅₀	7.1 nM Compound: 1	Effective concentration against human 5-hydroxytryptamine 1A receptor using cAMP as radioligand in CHO cells Effective concentration against human 5-hydroxytryptamine 1A receptor using cAMP as radioligand in CHO cells	[PMID: 15239661]
CHO	IC₅₀	7.1 nM Compound: 4, WAY-100635	Antagonist activity at human 5HT1A receptor expressed in CHO cells assessed as blockade of 8-OH-DPAT-induced inhibition of forskolin-stimulated increase in cAMP level Antagonist activity at human 5HT1A receptor expressed in CHO cells assessed as blockade of 8-OH-DPAT-induced inhibition of forskolin-stimulated increase in cAMP level	[PMID: 20443629]
HEK293-EBNA	IC₅₀	0.91 nM Compound: 1, WAY-100635	Displacement of [3H]8-OH-DPAT from human cloned 5HT1A receptor expressed in HEK293 EBNA cells after 1 hr by liquid scintillation counting Displacement of [3H]8-OH-DPAT from human cloned 5HT1A receptor expressed in HEK293 EBNA cells after 1 hr by liquid scintillation counting	[PMID: 21520940]
JAR	IC₅₀	> 4500 nM Compound: 1	Inhibition concentration against [3H]5-HT uptake by human serotonin transporter in JAR cells Inhibition concentration against [3H]5-HT uptake by human serotonin transporter in JAR cells	[PMID: 15239661]

In Vitro

The functional properties and binding affinities of WAY-100635 are evaluated in HEK 293 cells stably expressing dopamine D_2L or D_4.4 receptors^[1].
WAY-100635 displays 940, 370, and 16 nM binding affinities at D_2L, D₃, and D_4.2 receptors, respectively. Saturation analyses demonstrat that the K_d of [³H] WAY-100635 at D_4.2 receptors is 2.4 nM. WAY-100635 is potent agonist in HEK-D_4.4 cells with EC₅₀ of 9.7 nM. WAY-100635 possesses high affinity for D_4.4 receptor (3.3 nM) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

WAY-100635 (1 mg/kg; subcutaneous injection; male Sprague-Dawley rats) treatment abolishes the reduction of the severity of abstinence signs induced by Rhodiola rosea administration in nicotine-dependent rat^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (220-240 g)^[2]
Dosage:	1 mg/kg
Administration:	Subcutaneous injection (Pharmacokinetic study)
Result:	Reduced total abstinence score, increased immobility time and the burying behavior was increased.

분자량

422.56

화학식

C₂₅H₃₄N₄O₂

CAS No.

162760-96-5

Appearance

Solid-Liquid Mixture

Color

Off-white to light yellow

SMILES

O=C(N(C1=NC=CC=C1)CCN2CCN(CC2)C3=CC=CC=C3OC)C4CCCCC4

선적

Room temperature in continental US; may vary elsewhere.

보관

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 66.67 mg/mL (157.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3665 mL	11.8326 mL	23.6653 mL
5 mM	0.4733 mL	2.3665 mL	4.7331 mL
10 mM	0.2367 mL	1.1833 mL	2.3665 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.97%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (249 KB) HNMR (136 KB) RP-HPLC (169 KB) LCMS (236 KB)

Handling Instructions (2659 KB)

References

[1]. Chemel BR, et al. WAY-100635 is a potent dopamine D4 receptor agonist. Psychopharmacology (Berl). 2006 Oct;188(2):244-51. [Content Brief]

[2]. Mannucci C, et al. Serotonin involvement in Rhodiola rosea attenuation of nicotine withdrawal signs in rats. Phytomedicine. 2012 Sep 15;19(12):1117-24. [Content Brief]

[3]. Al Hussainy R, et al. Design, synthesis, radiolabeling, and in vitro and in vivo evaluation of bridgehead iodinatedanalogues of N-{2-[4-(2-methoxyphenyl)piperazin-1-yl]ethyl}-N-(pyridin-2-yl)cyclohexanecarboxamide (WAY-100635) as potential SPECT ligands for the 5-HT1A receptor. J Med Chem. 2011 May 26;54(10):3480-91. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3665 mL	11.8326 mL	23.6653 mL	59.1632 mL
	5 mM	0.4733 mL	2.3665 mL	4.7331 mL	11.8326 mL
	10 mM	0.2367 mL	1.1833 mL	2.3665 mL	5.9163 mL
	15 mM	0.1578 mL	0.7888 mL	1.5777 mL	3.9442 mL
	20 mM	0.1183 mL	0.5916 mL	1.1833 mL	2.9582 mL
	25 mM	0.0947 mL	0.4733 mL	0.9466 mL	2.3665 mL
	30 mM	0.0789 mL	0.3944 mL	0.7888 mL	1.9721 mL
	40 mM	0.0592 mL	0.2958 mL	0.5916 mL	1.4791 mL
	50 mM	0.0473 mL	0.2367 mL	0.4733 mL	1.1833 mL
	60 mM	0.0394 mL	0.1972 mL	0.3944 mL	0.9861 mL
	80 mM	0.0296 mL	0.1479 mL	0.2958 mL	0.7395 mL
	100 mM	0.0237 mL	0.1183 mL	0.2367 mL	0.5916 mL

WAY-100635 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WAY-100635162760-96-5WAY100635WAY 1006355-HT ReceptorDopamine ReceptorSerotonin Receptor5-hydroxytryptamine ReceptorInhibitorinhibitorinhibit

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WAY-100635

고객리뷰

Other Forms of WAY-100635:

WAY-100635 Related Antibodies

12 Publications Citing Use of MCE WAY-100635

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All 5-HT Receptor Isoform Specific Products:

View All Dopamine Receptor Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

WAY-100635 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

WAY-100635 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report