Bakuchiol (Synonyms: (S)-(+)-Bakuchiol)

Name: Bakuchiol
Brand: MedChemExpress
SKU: HY-N0235
Rating: 5.0 (2 reviews)

製品番号: HY-N0235 純度: 98.08%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Bakuchiol is a phytoestrogen that can be obtained from psoralen seeds. Bakuchiol has been proven to be a non-competitive inhibitor of multiple enzymes, including UDP-glucuronosyltransferase 2B7 (UGT2B7) and human carboxylesterase 2 (hCE2) , with IC₅₀s values of 40.9 μM and 7.28 μM, respectively. Bakuchiol exhibits significant research and application potential in areas such as anti-inflammatory, antibacterial, antitumor therapies, as well as drug metabolism regulation.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Bakuchiol 構造式

CAS 番号 : 10309-37-2

容量	価格（税別）	在庫状況	数量
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	在庫あり	Estimated Time of Arrival: December 31
Liquid
5 mg	$50	在庫あり	Estimated Time of Arrival: December 31
10 mg	$80	在庫あり	Estimated Time of Arrival: December 31
25 mg	$160	在庫あり	Estimated Time of Arrival: December 31
50 mg	$260	在庫あり	Estimated Time of Arrival: December 31
100 mg	$360	在庫あり	Estimated Time of Arrival: December 31
500 mg	$720	在庫あり	Estimated Time of Arrival: December 31
1 g		お問い合わせ
5 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of Bakuchiol:

Bakuchiol (Standard) お問い合わせ

顧客検証

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

CES1 CES2 CES3

p38 MAPK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Bakuchiol is a phytoestrogen that can be obtained from psoralen seeds. Bakuchiol has been proven to be a non-competitive inhibitor of multiple enzymes, including UDP-glucuronosyltransferase 2B7 (UGT2B7) ^[2] and human carboxylesterase 2 (hCE2) ^[3], with IC₅₀s values of 40.9 μM and 7.28 μM, respectively. Bakuchiol exhibits significant research and application potential in areas such as anti-inflammatory^[5], antibacterial^[4], antitumor^[1] therapies, as well as drug metabolism regulation.

IC₅₀ & Target

IC₅₀:40.9 nM (UGT2B7) ; IC₅₀:7.28 nM (hCE2)

Cellular Effect

Cell Line	Type	Value	Description	References
AGS	IC₅₀	15.3 μM Compound: 1	Cytotoxicity against human AGS cells by MTT assay Cytotoxicity against human AGS cells by MTT assay	[PMID: 18359631]
AGS	IC₅₀	6.1 μM Compound: 1	Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay	[PMID: 18359631]
BJ	EC₅₀	>36.63 μM Compound: 1	Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
HEK293	EC₅₀	>36.63 μM Compound: 1	Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
HeLa	IC₅₀	11 μM Compound: 1	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 18359631]
HeLa	IC₅₀	6.9 μM Compound: 1	Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells	[PMID: 18359631]
HepG2	EC₅₀	>52.0833 μM Compound: 1	Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
Jurkat	EC₅₀	>49.0196 μM Compound: 1	Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
MDA-MB-231	IC₅₀	8.29 x 10^-3mol/L Compound: 1	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 22245048]
MDA-MB-231	IC₅₀	8.29*10^-3M Compound: 1	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 22245048]
NALM-6	EC₅₀	38 μM Compound: 1	Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
Raji	EC₅₀	>26.0417 μM Compound: 1	Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
RAW264.7	IC₅₀	97.23 μM Compound: BAK	Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay	[PMID: 35000392]
SEM	EC₅₀	15.2866 μM Compound: 1	Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay	[PMID: 26922230]
T47D	IC₅₀	2.89 x 10^-5mol/L Compound: 1	Cytotoxicity against human T47D cells Cytotoxicity against human T47D cells	[PMID: 22245048]
T47D	IC₅₀	2.89*10^-5M Compound: 1	Cytotoxicity against human T47D cells Cytotoxicity against human T47D cells	[PMID: 22245048]

体外実験

Bakuchiol (5-20 μM, 72 h) exhibits selected cytotoxicity in A549 cells, as determined by the MTT assay. Bakuchiol displays stronger effect than its analogue Resveratrol (HY-16561) in reducing the viability of A549 cells with IC₅₀s vales of 9.58 μM and 33.02 μM, respectively^[1].
Bakuchiol (5-20 μM, 24 h or 36h) blocks cell cycle progression at S phase and induction of Reactive Oxygen species (ROS) -related apoptosis (more importantly) in A549 cells in a concentration-dependent manner^[1].
Bakuchiol (0-80 μM, 120 min) is a non-competitive inhibitor of UDP-glucuronosyltransferase 2B7 (UGT2B7) with K_i value of 10.7 μM and IC₅₀ value of 40.9 μM^[2].
Bakuchiol (30 min) is a potent non-competitive inhibitor of human carboxylesterase 2 (hCE2) with low K_i value of 2.12 μM and IC₅₀ value of 7.28 μM^[3].
Bakuchiol (0.9775-3.91 μg/mL, 24 h) demonstrates a significant dose-dependent increase in membrane permeability of the fungal conidia of T. mentagrophytes^[4].
Bakuchiol (3.91 μg/mL, 3 h) elicites a 187% elevation in Reactive Oxygen species (ROS) level in fungal cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:	5, 10, and 20 μM
Incubation Time:	24 hours
Result:	Induced caspase 9/3 activaton, p53 and Bax up-regulation, as well as Bcl-2 down-regulation.

Apoptosis Analysis^[1]

Cell Line:	A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:	5, 10, and 20 μM
Incubation Time:	36 hours
Result:	Resulted in typical apoptotic cells (p < 0.05), as determined by Annexin V/propidium iodide staining and flow cytometry.

Cell Cycle Analysis^[1]

Cell Line:	A549 cells
Concentration:	5, 10, and 20 μM
Incubation Time:	24 hours
Result:	Increased S phase cell population accompanied with a concomitant reduction of cells in the G1 phase. Resulted in a less potent effect in S phase arrest than bakuchiol.

Cell Cytotoxicity Assay^[1]

Cell Line:	A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration:	5, 10, and 20 μM
Incubation Time:	72 hours
Result:	Inhibited the growth of A549 cells, while the growth rate of other cells were not changed. Exhibited concentration-dependent cytotoxicity in A549 cells, while bakuchiol showed a more potent effect than that of resveratrol with IC₅₀s vales of 9.58 μmol/L and 33.02 μmol/ L, respectively.

体内実験

Bakuchiol (10 and 20 mg/kg, p.o.) can attenuate allergic symptoms, decrease the inflammatory response, improve the T-cell balance, reduce oxidative stress and regulated Igs levels in ovalbumin (OVA) -induced allergic rhinitis (AR) mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	OVA -induced AR model (BALB/c mice, 6-week-old) ^[5]
Dosage:	10 and 20 mg/kg
Administration:	Oral route (p.o.)
Result:	Reduced the nasal symptoms and decreased the levels of IL-4, IL-5, IL-13, Igs (IgE and IgG1), histamine, IL-10, IL-33, and TNF-α. Reduced PGDA and LTC-4 levels in the nasal lavage fluid (NLF). Decreased the ROS and MDA levels, whereas boosting SOD activity. Decreased the eosinophil count in the nasal tissues and influenced the Th1 and Th2 cell proportions.

臨床実験

分子量

256.38

分子式

C₁₈H₂₄O

CAS 番号

10309-37-2

Appearance

Liquid (Density: 0.963 g/cm³)

Color

Colorless to yellow

SMILES

OC1=CC=C(/C=C/[C@](C)(C=C)CC/C=C(C)\C)C=C1

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 62.5 mg/mL (243.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9005 mL	19.5023 mL	39.0046 mL
5 mM	0.7801 mL	3.9005 mL	7.8009 mL
10 mM	0.3900 mL	1.9502 mL	3.9005 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (8.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (8.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.25%

Select Batch:

データシート (278 KB) SDS (458 KB)

COA (243 KB) HNMR (176 KB) LCMS (523 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chen Z, et al. Anti-tumor effects of bakuchiol, an analogue of resveratrol, on human lung adenocarcinoma A549 cell line. Eur J Pharmacol. 2010 Sep 25;643(2-3):170-9. [Content Brief]

[2]. Xu Y, et al. In vitro evidence for bakuchiol's influence towards drug metabolism through inhibition of UDP-glucuronosyltransferase (UGT) 2B7. Afr Health Sci. 2014 Sep;14(3):564-9. [Content Brief]

[3]. Li YG, et al. Fructus Psoraleae contains natural compounds with potent inhibitory effects towards human carboxylesterase 2. Fitoterapia. 2015 Jan 13;101C:99-106. [Content Brief]

[4]. Lau KM, et al. Anti-dermatophytic activity of bakuchiol: in vitro mechanistic studies and in vivo tinea pedis-inhibiting activity in a guinea pig model. Phytomedicine. 2014 Jun 15;21(7):942-5. [Content Brief]

[5]. Yao HB, et al. Anti-Allergic and Anti-inflammatory Effects of Bakuchiol on Ovalbumin-Induced Allergic Rhinitis in Mice. Appl Biochem Biotechnol. 2024;196(6):3456-3470. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9005 mL	19.5023 mL	39.0046 mL	97.5115 mL
	5 mM	0.7801 mL	3.9005 mL	7.8009 mL	19.5023 mL
	10 mM	0.3900 mL	1.9502 mL	3.9005 mL	9.7512 mL
	15 mM	0.2600 mL	1.3002 mL	2.6003 mL	6.5008 mL
	20 mM	0.1950 mL	0.9751 mL	1.9502 mL	4.8756 mL
	25 mM	0.1560 mL	0.7801 mL	1.5602 mL	3.9005 mL
	30 mM	0.1300 mL	0.6501 mL	1.3002 mL	3.2504 mL
	40 mM	0.0975 mL	0.4876 mL	0.9751 mL	2.4378 mL
	50 mM	0.0780 mL	0.3900 mL	0.7801 mL	1.9502 mL
	60 mM	0.0650 mL	0.3250 mL	0.6501 mL	1.6252 mL
	80 mM	0.0488 mL	0.2438 mL	0.4876 mL	1.2189 mL
	100 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9751 mL

Bakuchiol Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

日本の代理先をお選びくださいナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
製品名			カスタマ需要量 *
お名前 *			タイトル
メールアドレス *			電話番号 *
デパートメント			組纖名 *
市区町村			都道府県
国或いは地域 *
必ず会社名を記載ください。個人への返信は行いません。
備考

製品名			Lot #
お名前 *			メールアドレス *
電話番号 *			部門 *
組纖名 *			国或いは地域 *
備考

Bakuchiol (Synonyms: (S)-(+)-Bakuchiol)

カスタマーレビュー

Other Forms of Bakuchiol:

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示:

p38 MAPK アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bakuchiol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

お名前
メール *

	Sorry, but the email address you supplied was invalid.

Bakuchiol (Synonyms: (S)-(+)-Bakuchiol)

カスタマーレビュー

Other Forms of Bakuchiol:

Bakuchiol 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Carboxylesterase (CES) アイソフォーム固有の製品をすべて表示:

p38 MAPK アイソフォーム固有の製品をすべて表示:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Bakuchiol 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bakuchiol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

カスタマーレビュー

Request for HNMR Report

Request for HNMR Report