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  4. Nitrofurazone

ニトロフラゾン  (Synonyms: Nitrofurazone; Nitrofural)

製品番号: HY-B0226 純度: 99.96%
COA 取扱説明書 Technical Support

Nitrofurazone (Nitrofural) is a broad spectrum antibiotic that has oral activity. Nitrofurazone is a nitro-aromatic drug. Nitrofurazone is active against both Gram-positive and Gram-negative bacteria.

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Nitrofurazone

ニトロフラゾン 構造式

CAS 番号 : 59-87-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
500 mg $30 在庫あり
1 g $40 在庫あり
5 g $100 在庫あり
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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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Based on 3 publication(s) in Google Scholar

Other Forms of Nitrofurazone:

ニトロフラゾン 関連抗体

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製品説明

Nitrofurazone (Nitrofural) is a broad spectrum antibiotic that has oral activity. Nitrofurazone is a nitro-aromatic drug. Nitrofurazone is active against both Gram-positive and Gram-negative bacteria[1][2][3][4][5].

Cellular Effect
Cell Line Type Value Description References
THP-1 IC50
> 100 μM
Compound: NFZ
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
[PMID: 36516584]
THP-1 IC50
> 100 μM
Compound: NFZ
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin staining based analysis
Cytotoxicity against human THP-1 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin staining based analysis
[PMID: 37859713]
V79 IC50
270 μM
Compound: Nitrofurazone
Differential hypoxic cytotoxicity towards chinese hamster cells (V79) using MTT assay under anaerobic conditions
Differential hypoxic cytotoxicity towards chinese hamster cells (V79) using MTT assay under anaerobic conditions
10.1016/0960-894X(95)00352-T
V79 IC50
740 μM
Compound: Nitrofurazone
Differential hypoxic cytotoxicity towards chinese hamster cells (V79) using MTT assay under aerobic conditions
Differential hypoxic cytotoxicity towards chinese hamster cells (V79) using MTT assay under aerobic conditions
10.1016/0960-894X(95)00352-T
Vero IC50
> 100 μM
Compound: NFZ
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based assay
[PMID: 36516584]
Vero IC50
> 100 μM
Compound: NFZ
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin staining based analysis
Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin staining based analysis
[PMID: 37859713]
体外実験

Nitrofurazone (10-20 μg/mL) can make E. coli strain B/r triple resistant mutants, increasing drug resistance by 6 to 7 times[2].
Nitrofurazone (50 μg/mL, 30 min) inhibits the synthesis of all color RNA and ribosomal subunits and the formation of polysomes in E. coli strain B/r[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ニトロフラゾン 関連抗体

RT-PCR[3]

Cell Line: E. Coli stratin B/r
Concentration: 50 μg/mL
Incubation Time: 30 min
Result: Inhibited the synthesis of 16 and 23s ribosomal RNA and 45 RNA.
体内実験

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Nitrofurazone can be used to create breast tumor models. The pharmacokinetic characteristics of Nitrofurazone in male white rabbits show a high clearance constant (0.04) and a short elimination half-life (17.32 minutes) with an AUC0-∞ of 844.79 when administered intravenously. When given orally, Nitrofurazone exhibits a lower clearance constant (0.002), a longer elimination half-life (276.09 minutes), and an AUC0-∞ of 566.44. After oral administration, the mean residence time of Nitrofurazone is 956.1 minutes, significantly longer than the 496.3 minutes observed after intravenous administration[4][5][6][7].

Induction of breast tumor[5]
Background
Nitrofurazone, with the involvement of cytochrome P450 reductase, generates metabolites that cause oxidative damage to the DNA of MCF-7 cells (human breast cancer-derived cells). At the same time, Amiodarone itself promotes cell proliferation and increases serum prolactin and progesterone levels in rats, thereby inducing breast cancer[4][5][6].
Specific Modeling Methods
Rat: Wistar • female • 4-week-old
Administration: diets containing 1000 ppm of Nitrofurazone • po • once daily, between 8 and 27 weeks of age
Note
Two-Stage Breast Cancer Induction Method:
1. At 7 weeks of age, each rat is administered a single dose of 20 mg of DMBA (9,10-dimethyl-1,2-benzanthracene) orally. DMBA is dissolved in sesame oil at a concentration of 2% before use.
2. From 8 to 27 weeks of age, each rat is given a diet containing 1000 ppm of Nitrofurazone daily (ppm: parts per million, indicating the amount of Nitrofurazone per million units of the diet; for example, a concentration of 1000 ppm means 1 g of Nitrofurazone in 1 kg of feed).
Modeling Indicators
Hormonal Changes: Significant increases in serum prolactin and progesterone levels in rats.
Histological Changes: Development of mammary tumors, with the vast majority being adenocarcinomas, and a very small number potentially being fibrosarcomas.
Correlated Product(s): DMBA (HY-W011845)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

198.14

分子式

C6H6N4O4
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N/N=C/C1=CC=C([N+]([O-])=O)O1)N
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 155 mg/mL (782.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.0469 mL 25.2347 mL 50.4694 mL
5 mM 1.0094 mL 5.0469 mL 10.0939 mL
10 mM 0.5047 mL 2.5235 mL 5.0469 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.58 mg/mL (13.02 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.58 mg/mL (13.02 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.96%

COA (246 KB) RP-HPLC (257 KB) MS (228 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.0469 mL 25.2347 mL 50.4694 mL 126.1734 mL
5 mM 1.0094 mL 5.0469 mL 10.0939 mL 25.2347 mL
10 mM 0.5047 mL 2.5235 mL 5.0469 mL 12.6173 mL
15 mM 0.3365 mL 1.6823 mL 3.3646 mL 8.4116 mL
20 mM 0.2523 mL 1.2617 mL 2.5235 mL 6.3087 mL
25 mM 0.2019 mL 1.0094 mL 2.0188 mL 5.0469 mL
30 mM 0.1682 mL 0.8412 mL 1.6823 mL 4.2058 mL
40 mM 0.1262 mL 0.6309 mL 1.2617 mL 3.1543 mL
50 mM 0.1009 mL 0.5047 mL 1.0094 mL 2.5235 mL
60 mM 0.0841 mL 0.4206 mL 0.8412 mL 2.1029 mL
80 mM 0.0631 mL 0.3154 mL 0.6309 mL 1.5772 mL
100 mM 0.0505 mL 0.2523 mL 0.5047 mL 1.2617 mL
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Nitrofurazone Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitrofurazone59-87-0NitrofuralEnvironmental PollutantsBacterialAntibioticEnvironmental ContaminantsInhibitorRNA synthesisF344/N ratsB6C3Fl miceinhibitorinhibit

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