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  5. DMBA

DMBA  (Synonyms: 7,12-DMBA; 7,12-Dimethylbenzanthracene)

Cat. No.: HY-W011845 Purity: 99.93%
COA Handling Instructions

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DMBA has carcinogenic activity as a polycyclic aromatic hydrocarbon (PAH). DMBA is used to induce tumor formation in various rodent models.

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DMBA Chemical Structure

DMBA Chemical Structure

CAS No. : 57-97-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

DMBA Related Antibodies

Top Publications Citing Use of Products

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  • Purity & Documentation

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Description

DMBA has carcinogenic activity as a polycyclic aromatic hydrocarbon (PAH). DMBA is used to induce tumor formation in various rodent models[1].

In Vivo

DMBA can be used in animal modeling to construct chemically induced skin carcinogenesis, breast cancer and other cancer models.
DMBA (0-150 mg/kg, p.o.) results in a decrease in spleen weight and in the total lymphocytes recovered from the spleen in C57BL/6 mice. DMBA exhibits a terminal deposition half-life of 45 min at all dose ranges (5-50 mg) in SD rats[5]. DMBA (0-150 mg/kg, p.o.) results in a decrease in spleen weight and in the total lymphocytes recovered from the spleen in C57BL/6 mice. DMBA exhibits a terminal deposition half-life of 45 min at all dose ranges (5-50 mg) in SD rats[5].
1. Induction of breast cancer[2]
Background
DMBA activates AhR, which translocates into nucleus and dimerizes with cofactor ARNT, thus induces the expressions of protooncogenes c-myc.
Specific Mmodeling Methods
Rat: Sprague-Dawley?•?female?•?50 days •140-150 g
Administration: 15-20 mg DMBA ?•?intragastric gavage?•?single dose
Note
1.5-2 g DMBA dissolved in 100 ml sesame oil


Modeling Indicators
Palpation:Detection of mammary cancer in 150 days.
Histology analysis:The tumor exhibits tightly spaced chords epithelial cells, cysts and papillary structures.
Phenotype:develops a temporary cessation in body growth.
Opposite Product(s): Phytic acid (HY-N0814); Limonene (HY-N0544)
2. Induction of skin cancer[3]
Background
DMBA is metabolized to reactive intermediates, such as syn- and anti-diol epoxides, and binds extensively to epidermal DNA.
Specific Mmodeling Methods
European hamster:?• 10 weeks • 180-200 g •
Administration: 25 mg DMBA • applied on the shaved back skin • single dose
Note
25 mg DMBA is dissolved in 1 ml acetone


Modeling Indicators
Histological analysis: Epidermal hyperplasia is oberserved. Lesions occurred frequently in the back skin, such as trichoepitheliomas, neoplastic epidermal cysts and melanotic tumors
Opposite Product(s): α-Mangostin (HY-N0328); DHEA (HY-14650); Salidroside (HY-N0109)
3. Induction of lung cancer[4]
Background
DMBA is metabolized by cytochrome P450 enzymes into the carcinogenic form 7,12-DMBA-3,4-dihydrodiol-1,2- epoxide, which adducts with DNA and produces mutations.
Specific Mmodeling Methods
BALB/c mice:?•12 weeks?• 25-35 g • 5-20 mg/kg • intratracheal instillation • single dose.
Note
(1). 5-20 mg DMBA is dissolved in 0.2 ml saline.
(2). A coat of antibiotic is applied after the instillation.


Modeling Indicators
Molecular changes:Increased number of macrophages in the broncho-alveolar lavage (BAL) fluid and levels of malonyldialdehyde (MDA) in lung tissue.
Histology analysis:Inflammation with alveolar epithelial hyperplasia, adenic metaplasia, adenomatoid hyperplasia and atypical hyperplasia.
Opposite Product(s): Diclofenac (HY-15036)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

256.34

Formula

C20H16
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(C1=CC=CC=C21)=C3C=CC4=CC=CC=C4C3=C2C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (97.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Acetone : 25 mg/mL (97.53 mM; Need ultrasonic)

Ethanol : 3.33 mg/mL (12.99 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9011 mL 19.5053 mL 39.0107 mL
5 mM 0.7802 mL 3.9011 mL 7.8021 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (9.75 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2 mg/mL (7.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

COA (251 KB) HNMR (247 KB) LCMS (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO / Acetone 1 mM 3.9011 mL 19.5053 mL 39.0107 mL 97.5267 mL
5 mM 0.7802 mL 3.9011 mL 7.8021 mL 19.5053 mL
10 mM 0.3901 mL 1.9505 mL 3.9011 mL 9.7527 mL
DMSO / Acetone 15 mM 0.2601 mL 1.3004 mL 2.6007 mL 6.5018 mL
20 mM 0.1951 mL 0.9753 mL 1.9505 mL 4.8763 mL
25 mM 0.1560 mL 0.7802 mL 1.5604 mL 3.9011 mL
30 mM 0.1300 mL 0.6502 mL 1.3004 mL 3.2509 mL
40 mM 0.0975 mL 0.4876 mL 0.9753 mL 2.4382 mL
50 mM 0.0780 mL 0.3901 mL 0.7802 mL 1.9505 mL
60 mM 0.0650 mL 0.3251 mL 0.6502 mL 1.6254 mL
80 mM 0.0488 mL 0.2438 mL 0.4876 mL 1.2191 mL
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DMBA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMBA57-97-67,12-DMBA 7,12-DimethylbenzanthraceneOthersInhibitorinhibitorinhibit

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