2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras p38 MAPK JNK
  4. RBC8

RBC8 is a selective and allosteric RALA and RALB inhibitor. RBC8 stabilizes the inactive GDP-bound state of Ral, preventing its activation. RBC8 promotes the phosphorylation of proteins related to the MAPK/JNK pathway. RBC8 has the activity of inhibiting tumor cell proliferation, migration and invasion. RBC8 is used in the study of various cancers such as lung cancer, gastric cancer, and multiple myeloma.

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RBC8

RBC8 構造式

CAS 番号 : 361185-42-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 126 在庫あり
Solution
10 mM * 1 mL in DMSO USD 126 在庫あり
Solid
5 mg $115 在庫あり
10 mg $195 在庫あり
25 mg $380 在庫あり
50 mg $600 在庫あり
100 mg $960 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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RBC8 関連抗体

Top Publications Citing Use of Products

Ras アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
K-Ras H-Ras N-Ras Ras

p38 MAPK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

JNK アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
JNK1 JNK2 JNK3 JNK

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製品説明

RBC8 is a selective and allosteric RALA and RALB inhibitor. RBC8 stabilizes the inactive GDP-bound state of Ral, preventing its activation. RBC8 promotes the phosphorylation of proteins related to the MAPK/JNK pathway. RBC8 has the activity of inhibiting tumor cell proliferation, migration and invasion. RBC8 is used in the study of various cancers such as lung cancer, gastric cancer, and multiple myeloma[1][2][3].

体外実験

RBC8 (10-20 μM; 3-72 h) can reduce the survival rate of myeloma cells AMO-1 and INA-6 and the level of RAL-GTP in sensitive INA-6 cells[1]. RBC8 (20 μM; 3 days of treatment) inhibits the colony formation of lung cancer cells H2122 and H358 in soft agar with IC50 of 3.5 μM and 3.4 μM, respectively[2]. RBC8 (20-40 μM) significantly inhibits the growth, migration and invasion of gastric cancer cell lines SGC-7901 and MGC-803[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RBC8 関連抗体
体内実験

RBC8 (50 mg/kg; i.p.; daily (except weekends); 21 days) inhibits tumor growth in nude mice inoculated with H2122 and H358 human lung cancer cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female athymic nude mice (Ncr nu/nu; 5 - 6 weeks old, weight not specified) inoculated with H2122 or H358 human lung cancer cells[2].
Dosage: 50 mg/kg (dissolved in DMSO)
Administration: Intraperitoneal injection, daily except weekends, for 21 days
Result: Inhibited the growth of xenograft tumors in nude mice inoculated with H2122 cells, and the effect was similar to that of dual knockdown of RalA and RalB.
Inhibited the growth of xenograft tumors in nude mice inoculated with H358 cells.
分子量

424.45

分子式

C25H20N4O3
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

N#CC(C1C2=CC(OC)=CC=C2OC)=C(N)OC3=C1C(C4=CC=C5C=CC=CC5=C4)=NN3
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 40 mg/mL (94.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3560 mL 11.7800 mL 23.5599 mL
5 mM 0.4712 mL 2.3560 mL 4.7120 mL
10 mM 0.2356 mL 1.1780 mL 2.3560 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (5.89 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3560 mL 11.7800 mL 23.5599 mL 58.8998 mL
5 mM 0.4712 mL 2.3560 mL 4.7120 mL 11.7800 mL
10 mM 0.2356 mL 1.1780 mL 2.3560 mL 5.8900 mL
15 mM 0.1571 mL 0.7853 mL 1.5707 mL 3.9267 mL
20 mM 0.1178 mL 0.5890 mL 1.1780 mL 2.9450 mL
25 mM 0.0942 mL 0.4712 mL 0.9424 mL 2.3560 mL
30 mM 0.0785 mL 0.3927 mL 0.7853 mL 1.9633 mL
40 mM 0.0589 mL 0.2945 mL 0.5890 mL 1.4725 mL
50 mM 0.0471 mL 0.2356 mL 0.4712 mL 1.1780 mL
60 mM 0.0393 mL 0.1963 mL 0.3927 mL 0.9817 mL
80 mM 0.0294 mL 0.1472 mL 0.2945 mL 0.7362 mL
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RBC8 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RBC8361185-42-4RBC 8RBC-8Rasp38 MAPKJNKc-Jun N-terminal kinaseInhibitorinhibitorinhibit

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製品名:
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製品番号:
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