Diclazuril (Synonyms: R-64433)

Name: Diclazuril
Brand: MedChemExpress
SKU: HY-B0357
Rating: 5.0 (2 reviews)

Art. -Nr.: HY-B0357 Reinheit: 99.70%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Diclazuril (R-64433), a benzeneacetonitrile derivative, is a potent and orally active anticoccidial agent. Diclazuril can be used for the research of certain infectious and parasitic diseases, including coccidiosis, acute toxoplasmosis, equine protozoal pyoencephalitis (EPM) et.al.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Diclazuril Chemische Struktur

CAS. Nr. : 101831-37-2

Größe	Verfügbarkeit	Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
50 mg	Auf Lager	Estimated Time of Arrival: December 31
100 mg	Auf Lager	Estimated Time of Arrival: December 31
500 mg	Auf Lager	Estimated Time of Arrival: December 31
1 g	Auf Lager	Estimated Time of Arrival: December 31
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This product is a controlled substance and not for sale in your territory.

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Other Forms of Diclazuril:

Diclazuril-d₄ In-stock
Diclazuril-¹³C₃,¹⁵N₂ Angebot einholen
Diclazuril potassium Angebot einholen
Diclazuril sodium Angebot einholen
Diclazuril (Standard) Angebot einholen

2 Publications Citing Use of MCE Diclazuril

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Parasite Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Alle Antibiotic Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Biologische Aktivität
Reinheit & Dokumentation
Verweise
Kundenbewertung

Beschreibung

IC₅₀ & Target

Coccidia

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	31.6 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	31.6 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
Hepatocyte	IC₅₀	0.008 6 Compound: 15	In vitro anti-coccidial activity against chicken hepatocyte Eimeria acervulina In vitro anti-coccidial activity against chicken hepatocyte Eimeria acervulina	[PMID: 10476881]
HepG2	EC₅₀	35.5 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	35.5 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	31.6 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	9 3 Compound: Diclazuril	Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	9 3 Compound: Diclazuril	Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	35.5 3 Compound: Diclazuril	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
MDCK	EC₅₀	31 3 Compound: 22	Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay	[PMID: 27046062]
HepG2	EC₅₀	9 3 Compound: Diclazuril	Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
MDCK	EC₅₀	31 3 Compound: 4	Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay	[PMID: 25856229]
MDCK	EC₅₀	31 3 Compound: 4	Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay	[PMID: 25856229]
MDCK	EC₅₀	31 3 Compound: 22	Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay	[PMID: 27046062]
Hepatocyte	IC₅₀	0.0008 102 Compound: Diclazuril	Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the absence of inhibitor; value given as 0.004-0.0008 Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the absence of inhibitor; value given as 0.004-0.0008	[PMID: 9873375]
Hepatocyte	IC₅₀	0.0008 102 Compound: Diclazuril	Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the presence of Tranylcypromine; value given as 0.004-0.0008 Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the presence of Tranylcypromine; value given as 0.004-0.0008	[PMID: 9873375]
Hepatocyte	IC₅₀	0.0008 102 Compound: Diclazuril	Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the presence of iproniazid; value given as 0.004-0.0008 Inhibitory activity against second generation merozoids of Eimeria tenella in chick hepatocyte assay in the presence of iproniazid; value given as 0.004-0.0008	[PMID: 9873375]
Hepatocyte	IC₅₀	0.008 6 Compound: 15	In vitro anti-coccidial activity against chicken hepatocyte Eimeria acervulina In vitro anti-coccidial activity against chicken hepatocyte Eimeria acervulina	[PMID: 10476881]
Hepatocyte	IC₅₀	0.008 6 Compound: 15	In vitro anti-coccidial activity against chicken hepatocyte Eimeria tenella In vitro anti-coccidial activity against chicken hepatocyte Eimeria tenella	[PMID: 10476881]
MDCK	EC₅₀	31 3 Compound: 22	Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay Antiviral activity against Influenza A virus A/PR/8/34 (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 2 days by plaque reduction assay	[PMID: 27046062]
MDCK	EC₅₀	31 3 Compound: 4	Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay Antiviral activity against Influenza A virus (A/PR/8/34) (H1N1) infected in MDCK cells assessed as reduction in plaque formation after 48 hrs by plaque reduction assay	[PMID: 25856229]

In Vivo

Diclazuril (R-64433) is a coccidiostat, the lambs treated with diclazuril showed an intense, persistent oocyst excretion, with average levels of 97.54 opg, considerably higher than those recorded in the animals treated with toltrazuril (p < 0.05)^[1]. Diclazuril (R-64433) is effective against E. maxima later in its life cycle, subclinical intestinal lesions may be present for a short time after infection. Diclazuril (R-64433) was shown in studies to reduce lesion scores and improve performance and health of birds challenged with E. maxima^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Klinische Studie

Molekulargewicht

407.64

Formel

C₁₇H₉Cl₃N₄O₂

CAS. Nr.

101831-37-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=O)N(N=C1)C2=CC(Cl)=C(C(C#N)C3=CC=C(Cl)C=C3)C(Cl)=C2

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (61.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4531 mL	12.2657 mL	24.5314 mL
5 mM	0.4906 mL	2.4531 mL	4.9063 mL
10 mM	0.2453 mL	1.2266 mL	2.4531 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 99.70%

Select Batch:

Data Sheet (280 KB) SDS (399 KB)

COA (258 KB) HNMR (257 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Diaferia, M., et al., Efficacy of toltrazuril 5 % suspension (Baycox(R), Bayer) and diclazuril (Vecoxan(R), Janssen-Cilag) in the control of Eimeria spp. in lambs. Parasitol Res, 2013. 112 Suppl 1: p. 163-8. [Content Brief]

[2]. http://www.huvepharma.com/products/view/190

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4531 mL	12.2657 mL	24.5314 mL	61.3286 mL
	5 mM	0.4906 mL	2.4531 mL	4.9063 mL	12.2657 mL
	10 mM	0.2453 mL	1.2266 mL	2.4531 mL	6.1329 mL
	15 mM	0.1635 mL	0.8177 mL	1.6354 mL	4.0886 mL
	20 mM	0.1227 mL	0.6133 mL	1.2266 mL	3.0664 mL
	25 mM	0.0981 mL	0.4906 mL	0.9813 mL	2.4531 mL
	30 mM	0.0818 mL	0.4089 mL	0.8177 mL	2.0443 mL
	40 mM	0.0613 mL	0.3066 mL	0.6133 mL	1.5332 mL
	50 mM	0.0491 mL	0.2453 mL	0.4906 mL	1.2266 mL
	60 mM	0.0409 mL	0.2044 mL	0.4089 mL	1.0221 mL

Diclazuril Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diclazuril101831-37-2R-64433R64433R 64433ParasiteAntibioticToxoplasma gondiicoccidiosisequine protozoal pyoencephalitisEPMparasiticanticoccidial agentSarcocystis neurona?parasiteEimeria tenellaInhibitorinhibitorinhibit

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Diclazuril (Synonyms: R-64433)

Kundenbewertung

Other Forms of Diclazuril:

Diclazuril Related Antibodies

2 Publications Citing Use of MCE Diclazuril

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Alle Parasite Isoform-spezifische Produkte anzeigen:

Alle Antibiotic Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Diclazuril Related Classifications

Keywords:

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SAFETY DATA SHEET (SDS)

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