2. Immunology/Inflammation
  3. COX NO Synthase
  4. Harpagoside

Harpagoside can be obtained by Harpagophytum procumbens, which has anti-inflammatory, anti-cancer, protective activity, and efficacy. Harpagoside has an inhibitory effect on COX-1 and COX-2 active, and suppresses NO production. Harpagoside inhibits HepG2 cell lipid polysaccharide, which is a protein that is expressed horizontally and selectively, and has anti-inflammatory and latent pain effects. Harpagoside has the ability to protect the body, and has a degenerative effect on the β-oxidation (Aβ).

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Harpagoside

Harpagoside 構造式

CAS 番号 : 19210-12-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 76 在庫あり
Solution
10 mM * 1 mL in DMSO USD 76 在庫あり
Solid
5 mg $70 在庫あり
10 mg $110 在庫あり
20 mg $180 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of Harpagoside:

Harpagoside 関連抗体

Top Publications Citing Use of Products

COX アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
COX COX-1 COX-2 COX-3

NO Synthase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
nNOS iNOS eNOS

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Harpagoside can be obtained by Harpagophytum procumbens, which has anti-inflammatory, anti-cancer, protective activity, and efficacy. Harpagoside has an inhibitory effect on COX-1 and COX-2 active, and suppresses NO production. Harpagoside inhibits HepG2 cell lipid polysaccharide, which is a protein that is expressed horizontally and selectively, and has anti-inflammatory and latent pain effects. Harpagoside has the ability to protect the body, and has a degenerative effect on the β-oxidation (Aβ).

IC50 & Target[1]

COX-1

 

COX-2

 

Cellular Effect
Cell Line Type Value Description References
HUVEC IC50
88.2 3
Compound: Harpagoside
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
[PMID: 20621474]
HUVEC IC50
88.2 3
Compound: Harpagoside
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
[PMID: 20621474]
HUVEC IC50
88.2 3
Compound: Harpagoside
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
Antiinflammatory activity in HUVEC assessed as inhibition of TNF-alpha-induced E-selection expression after 2 hrs by ELISA
[PMID: 20621474]
体外実験

Harpagoside (200 μM; 2 h+1 h, 6 h) inhibits lipopolysaccharide (100 ng/mL LPS)-induced mRNA levels and protein expression of cyclooxygenase 2 and inducible nitric oxide in HepG2 cells[2].
Harpagoside (0.1-200 μM; 4 h+1 h, 6 h) dose-dependently inhibits LPS-stimulated NF-κB promoter activity and interferes with the activation of gene transcription[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Harpagoside 関連抗体
体内実験

Harpagoside (5 mg/kg; 15 mg/kg; po; once daily for 60 days) has potential neuroprotective effects against Aβ intoxication in an Aβ1-40-induced rat Alzheimer's disease (AD) model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

494.49

分子式

C24H30O11
CAS 番号
Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1[C@H](O)[C@@H](O)[C@]([H])(O[C@@H]2OC=C[C@]3(O)[C@@]2([H])[C@@](C)(OC(/C=C/C4=CC=CC=C4)=O)C[C@H]3O)O[C@@H]1CO
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 62.5 mg/mL (126.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0223 mL 10.1114 mL 20.2229 mL
5 mM 0.4045 mL 2.0223 mL 4.0446 mL
10 mM 0.2022 mL 1.0111 mL 2.0223 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (12.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.25 mg/mL (12.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.88%

COA (255 KB) HNMR (288 KB) LCMS (93 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0223 mL 10.1114 mL 20.2229 mL 50.5571 mL
5 mM 0.4045 mL 2.0223 mL 4.0446 mL 10.1114 mL
10 mM 0.2022 mL 1.0111 mL 2.0223 mL 5.0557 mL
15 mM 0.1348 mL 0.6741 mL 1.3482 mL 3.3705 mL
20 mM 0.1011 mL 0.5056 mL 1.0111 mL 2.5279 mL
25 mM 0.0809 mL 0.4045 mL 0.8089 mL 2.0223 mL
30 mM 0.0674 mL 0.3370 mL 0.6741 mL 1.6852 mL
40 mM 0.0506 mL 0.2528 mL 0.5056 mL 1.2639 mL
50 mM 0.0404 mL 0.2022 mL 0.4045 mL 1.0111 mL
60 mM 0.0337 mL 0.1685 mL 0.3370 mL 0.8426 mL
80 mM 0.0253 mL 0.1264 mL 0.2528 mL 0.6320 mL
100 mM 0.0202 mL 0.1011 mL 0.2022 mL 0.5056 mL
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Harpagoside Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Harpagoside19210-12-9COXNO SynthaseCyclooxygenaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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製品名:
Harpagoside
製品番号:
HY-N0396
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