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  3. Immunology and Inflammatory Disease Models Environmental Pollutants NO Synthase Endogenous Metabolite
  4. Pancreatitis Models
  5. L-Arginine

L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis.

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No. CAS : 74-79-3

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Based on 23 publication(s) in Google Scholar

Other Forms of L-Arginine:

L-Arginine Related Antibodies

Top Publications Citing Use of Products

    L-Arginine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e10500.  [Abstract]

    Intracellular ROS and NO generation were measured under different L-Arg (25-200 mg/mL) concentrations following iRGD NPs@si-SOX9/CL+L (24 h) treatment using DCFH-DA and DAF-FM DA probes

    L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333.  [Abstract]

    Images and tumor volume in subcutaneous tumors established with AQP5+ or AQP5− MFC cells in C57BL/6J mice treated with daily oral gavage of different concentrations of L-Arginine (1-2 g/kg; p.o. daily for 27 d) or PBS.

    L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333.  [Abstract]

    Proportion of NK cells in subcutaneous tumors detected by flow cytometry (AQP5+); AQP5+ + L-Arginine (1 g/kg daily oral gavage); AQP5+ + L-Arginine (2 g/kg daily oral gavage); AQP5-).

    L-Arginine purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 16;6(9):102333.  [Abstract]

    NK cells co-cultured with AQP5+ AGS cells, AQP5- AGS cells, or AQP5+ AGS cells supplemented with 40 nM L-Arginine for 48 h and co-cultured with AGS cells. Images detected by live cell workstation; NK cells were labeled green by CFSE, and AGS cells were labeled red.

    L-Arginine purchased from MedChemExpress. Usage Cited in: Nutrients. 2023 Oct 18;15(20):4427.

    A ketogenic diet exacerbated L-Arginine (4 g/kg; hourly i.p. for 2 times )-induced acute pancreatitis. H&E staining and TEM sections of pancreatic tissue.

    Ver todos los productos específicos de isoformas NO Synthase:

    Ver todas las isoformas
    nNOS iNOS eNOS

    Ver todos los productos específicos de isoformas Endogenous Metabolite:

    Ver todas las isoformas
    Human Endogenous Metabolite Microbial Metabolite

    • Actividad biológica

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    • Referencias

    • Revisión del cliente

    Descripciòn

    L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis[1][2][3][4][5].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    eNOS

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    283 3
    Compound: 6322
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells
    		10.5281/zenodo.6782708
    HEK293 EC50
    283 3
    Compound: 6322
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
    		10.5281/zenodo.6782708
    HEK293 EC50
    283 3
    Compound: 6322
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
    Substrate uptake by the Cationic Amino Acid Transporter (CAT-3, SLC7A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC7A3 cells (PubChem AID: 1745863)
    		10.5281/zenodo.6782708
    In Vitro

    Arginine is an α-amino acid. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that code for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual[1].
    L-Arginine is associated with a decrease in cardiac index. L-Arginine (450 mg/kg during a 15-minute period) amplifies and sustains the hyperemia (38%) and increases absolute brain blood flow after eNOS upregulation by chronic Simvastatin (HY-17502) (2 mg/kg; sc; daily for 14 days) in SV-129 mice[2][3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Arginine Related Antibodies
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    L-Arginine has been widely accepted as a method to induce experimental acute pancreatitis[4][5].
    Dose reference for L-Arginine induction[4][5]:
    (1) Model animal: Male Wistar albino rats
    Acute pancreatitis: single i.p. injection of 500 mg of L-arginine/100 g body weight
    (2) Model animal: Male ICR mice
    Acute pancreatitis: i.p. with 2.25 g/kg body weight of L-Arginine hourly for 2 hours
    Dissolution method of L-Arginine[5]:
    L-arginine solution was prepared by dissolving L-arginine powder in 0.9% normal saline and adjusting the pH to 7 with 5 N HCl.
    Induction of Experimental Acute Pancreatitis[4][5]
    Background
    L-Arginine is a substrate for endothelial nitric oxide synthase (eNOS) to produce NO, which can be metabolized into nitric oxide (NO), polyamines or L-proline, stimulating inflammatory responses. L-Arginine can also selectively destroy pancreatic acinar cells, leading to acute necrotizing pancreatitis.
    Specific Modeling Methods
    Rat[4]: Wistar albino rats • Male • 150 g
    Administration: 250 mg, 500 mg, 750 mg every 100 g body weight • ip • single dose • starved for 15 hours
    Mice[5]: ICT mouse • Male • 4-week-old • 25-30 g
    Administration: 450 mg/100 g body weight • ip • twice dosages • sacrificed at 72 hours
    Note
    (1) When the dose exceeds 500 mg/100 g body weight, most rats will die within a few hours after injection; When the dose is less than 250 mg, the induced symptoms of pancreatic disease will be mild[4].
    (2) Dissolution method in Reference: L-Arginine solution was prepared by dissolving L-Arginine powder in 0.9% normal saline and adjusting pH to 7 with 5N hydrochloric acid[5].
    Modeling Indicators
    Phenotypic changes: weight loss, lower activity, softer hair.
    Tissue morphology: pancreatic edema, damage, atrophy, granulation tissue contraction and chalky spots (location of fat necrosis).
    Cellular changes: basophils decrease and vesicles, cell pyknosis, nuclear fragmentation. Pancreatic albino cells disappear and die, and pancreatic tissue is replaced by monocytes and fibroblasts.
    Molecular changes: serum amylase levels increase, and fat necrosis is obvious.
    Correlated Product(s): Curcumin (HY-N0005)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Peso molecular

    174.20

    Fòrmula

    C6H14N4O2
    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CCCNC(N)=N)C(O)=O
    Structure Classification
    Initial Source
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    Store at room temperature 3 years

    In solvent -80°C 2 years
    -20°C 1 year
    Solvente y solubilidad
    In Vitro: 

    H2O : 50 mg/mL (287.03 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7405 mL 28.7026 mL 57.4053 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (574.05 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureza y Documentación

    Purity: 99.89%

    SDS (394 KB)

    COA (242 KB) HNMR (234 KB) RP-HPLC (242 KB) MS (68 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
    10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
    15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
    20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
    25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
    30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
    40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
    50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
    60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
    80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
    100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Arginine Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Arginine74-79-3Environmental PollutantsNO SynthaseEndogenous MetaboliteEnvironmental ContaminantsNitric oxide synthasesNOSNOvascularsmoothmuscletransporterInhibitorinhibitorinhibit

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