2. NF-κB Immunology/Inflammation Metabolic Enzyme/Protease Autophagy
  3. Endogenous Metabolite Reactive Oxygen Species (ROS) HIF/HIF Prolyl-Hydroxylase Autophagy
  4. Glucosamine hydrochloride

Glucosamine hydrochloride  (Synonyms: D-(+)-Glucosamine hydrochloride; Chitosamine hydrochloride)

Cat. No.: HY-N0733 Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

Glucosamine hydrochloride (D-Glucosamine hydrochloride) is an amino sugar and a prominent precursor in the biochemical synthesis of glycosylated proteins and lipids, is used as a dietary supplement. Glucosamine hydrochloride also is a natural constituent of glycosaminoglycans in the cartilage matrix and synovial fluid, which when administered exogenously, exerts pharmacological effects on osteoarthritic cartilage and chondrocytes.

For research use only. We do not sell to patients.

CAS No. : 66-84-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Glucosamine hydrochloride:

Glucosamine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Glucosamine hydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 24;11(12):5650-5674.  [Abstract]

    LUAD cells were treated with erastin with or without Fer-1 or DFO, in the presence or absence of GlcN (5 mM). Cell death was measured after treatment for 24 h.

    Glucosamine hydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 24;11(12):5650-5674.  [Abstract]

    LUAD cells were treated with erastin with or without Fer-1 or DFO, in the presence or absence of GlcN (5 mM). MDA was measured after treatment for 16 h.

    Glucosamine hydrochloride purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 24;11(12):5650-5674.  [Abstract]

    Metabolites downstream of GFPT1 restore YAP alteration by erastin. p-YAPS127, O-YAPT241 and YAP were measured by IB in H1975 cells treated with or without erastin, glucose, GlcN (5 mM) or GlcNAc for 8 h.

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    Human Endogenous Metabolite Microbial Metabolite

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    HIF-1α HIF

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Glucosamine hydrochloride (D-Glucosamine hydrochloride) is an amino sugar and a prominent precursor in the biochemical synthesis of glycosylated proteins and lipids, is used as a dietary supplement. Glucosamine hydrochloride also is a natural constituent of glycosaminoglycans in the cartilage matrix and synovial fluid, which when administered exogenously, exerts pharmacological effects on osteoarthritic cartilage and chondrocytes[1].

    IC50 & Target[1]

    Human Endogenous Metabolite

     

    Microbial Metabolite

     

    In Vitro

    Glucosamine hydrochloride (D-Glucosamine hydrochloride) exhibits dose-dependent DPPH antioxidant activity[2].
    Glucosamine hydrochloride treatment of Short-term (4 h) inhibits HIF-1α at the protein level, decreases phosphorylation of p70S6K and S6, translation-related proteins[3].
    Glucosamine hydrochloride significantly decreases renal expression of α-smooth muscle actin, collagen I, and fibronectin in the obstructed kidneys and TGF-β1-treated renal cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Glucosamine hydrochloride Related Antibodies
    Molecular Weight

    215.63

    Formula

    C6H14ClNO5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C[C@H](N)[C@H]([C@@H]([C@@H](CO)O)O)O.Cl
    Structure Classification
    Initial Source

    crustaceans and other arthropods, fungi
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (463.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 50 mg/mL (231.88 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.6376 mL 23.1879 mL 46.3757 mL
    5 mM 0.9275 mL 4.6376 mL 9.2751 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (11.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (11.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (463.76 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (246 KB) HNMR (252 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.6376 mL 23.1879 mL 46.3757 mL 115.9393 mL
    5 mM 0.9275 mL 4.6376 mL 9.2751 mL 23.1879 mL
    10 mM 0.4638 mL 2.3188 mL 4.6376 mL 11.5939 mL
    15 mM 0.3092 mL 1.5459 mL 3.0917 mL 7.7293 mL
    20 mM 0.2319 mL 1.1594 mL 2.3188 mL 5.7970 mL
    25 mM 0.1855 mL 0.9275 mL 1.8550 mL 4.6376 mL
    30 mM 0.1546 mL 0.7729 mL 1.5459 mL 3.8646 mL
    40 mM 0.1159 mL 0.5797 mL 1.1594 mL 2.8985 mL
    50 mM 0.0928 mL 0.4638 mL 0.9275 mL 2.3188 mL
    60 mM 0.0773 mL 0.3865 mL 0.7729 mL 1.9323 mL
    80 mM 0.0580 mL 0.2898 mL 0.5797 mL 1.4492 mL
    100 mM 0.0464 mL 0.2319 mL 0.4638 mL 1.1594 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Glucosamine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Glucosamine66-84-2D-(+)-GlucosamineChitosamineEndogenous MetaboliteReactive Oxygen Species (ROS)HIF/HIF Prolyl-HydroxylaseAutophagyHypoxia-inducible factorsHIFsHIF-PHInhibitorinhibitorinhibit

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