Spermine (Synonyms: NSC 268508; Neuridine)

Name: Spermine
Brand: MedChemExpress
SKU: HY-B1777
Rating: 5.0 (8 reviews)

Cat. No.: HY-B1777 Purity: 99.81%: Data Sheet
COA

SDS
Handling Instructions
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Spermine is a natural antioxidant and anti-inflammatory agent. Spermine is known to inhibit some bacterial cultures, especially strains of Staphylococcus aureus. Spermine induces neurotoxicity in the striarum dose-dependently. Spermine can reversibly inhibits DNA synthetic response, mixed lymphocyte response and the induction of cytolytic lymphocyte response in primary cultures of murine spleen cells. Spermine tetrahydrochloride is a polyamine nitric oxide donor that can provide nitric oxide to platelets and inhibit platelet activation to a certain extent concentration-dependently. Spermine tetrahydrochloride occurs in mammalian tissues, plants, bacteria, ribosomes and bacteriophage. Spermine tetrahydrochloride inhibits primary human embryo lung fibroblasts in vitro.

For research use only. We do not sell to patients.

CAS No. : 71-44-3

Size	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Spermine:

8 Publications Citing Use of MCE Spermine

In Vivo Efficacy Study

Bio/Physico-chemical Assay

Cell Imaging/Staining

: Spermine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]; Immunofluorescence showed the expression of JunD in basal cells before and within 9 days after irradiation (Spermine, 10 mg/kg, ip, 16 days).

: Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]; ThT kinetics of α-synuclein aggregation in the presence of different concentrations of Spermine (0-10 mM).

: Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]; Statistical analysis was performed on the maximum fluorescence intensity (Fmax) of the kinetic curve after 99 hours of Spermine (3.2-16 μM) incubation.

: Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]; Spermine (5 mg/kg, ip, single dose) treatment failed to alleviate no-reflow phenomenon and enhance microvascular perfusion.

: Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]; The beneficial effects of HINT2 on nitrite levels, eNOS expression, and eNOS phosphorylation were completely suppressed after Spermine (10 μM, 2 h) treatment.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	> 100 μM Compound: Spm, spermine	Cytotoxicity against CHO cells after 48 hrs by MTT assay Cytotoxicity against CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO	IC₅₀	> 100 μM Compound: Spm, spermine	Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay	[PMID: 18558490]
CHO-MG	IC₅₀	> 100 μM Compound: 3, spermine	Inhibition of cell growth in CHO-MG cells in the presence of DFMO Inhibition of cell growth in CHO-MG cells in the presence of DFMO	[PMID: 16392808]
Fibroblast	IC₅₀	14.6 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	14.9 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	356.7 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	422.1 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	> 500 μM Compound: Spermine	Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
Fibroblast	IC₅₀	> 500 μM Compound: Spermine	Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs	[PMID: 34695351]
HeLa	IC₅₀	50 μM Compound: spermine	Ability to inhibit 50% growth of HeLa cells Ability to inhibit 50% growth of HeLa cells	[PMID: 1875339]
HeLa	IC₅₀	70 μM Compound: spermine	Ability to cause aggregation of purified HeLa cell DNA Ability to cause aggregation of purified HeLa cell DNA	[PMID: 1875339]
Huh-7	CC₅₀	> 50 μM Compound: Spermine	Cytotoxicity against human HuH7 cells Cytotoxicity against human HuH7 cells	[PMID: 24177358]
Huh-7	EC₅₀	> 50 μM Compound: Spermine	Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy	[PMID: 24177358]
L1210	IC₅₀	0.39 μM Compound: Spermine	Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake. Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake.	[PMID: 9871574]
L1210	IC₅₀	54.5 μM Compound: Spm, spermine	Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay	[PMID: 18558490]

In Vitro

Spermine (40-100 μg/mL, 5 d) has ID₅₀ of 25 and 35 in H.E.L. cells and H.Ep.2 cells, respectively^[2].
Spermine (10 μg/well, when added up to 29 h after inception of culture) can inhibit the bacterial Lipopolysaccharide (HY-D1056) in BALB/c spleen cells^[3].
Spermine (5-10 μg/well, when added within 22 h after inception of culture) can completely inhibit concanavalin A (HY-P2149) in BALB/c spleen cells^[3].
Spermine (0.5 mM) shows 50% inhibition of peroxidation reaction in brain phospholipid liposomes^[4].
Spermine tetrahydrochloride (10-50 mM) increases the concentration of NO in platelets and inhibits the expression of CD62p concentration-dependently^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Spermine (50-250 nM, intrastriatally) produces severe cell loss and degeneration as well as neuronal damage around the site of injection^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

202.34

Formula

C₁₀H₂₆N₄

CAS No.

71-44-3

Appearance

<28°C Solid,>30°C Liquid

Color

Colorless to off-white

SMILES

NCCCNCCCCNCCCN

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 200 mg/mL (988.44 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9422 mL	24.7109 mL	49.4218 mL
5 mM	0.9884 mL	4.9422 mL	9.8844 mL
10 mM	0.4942 mL	2.4711 mL	4.9422 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (270 KB) SDS (477 KB)

COA (239 KB) HNMR (217 KB) MS (68 KB) GC (178 KB)

Handling Instructions (2659 KB)

References

[1]. U. Bachrach & B. Reches, (1966) Enzymic assay for spermine and spermidine, Analytical Biochemistry, Volume 17, Issue 1, Volume 17, Issue 1, ISSN 0003-2697.

[2]. Dawson, M., & Dryden, W. F. (1969). The toxicity of spermine and spermidine to cells in culture. Biochemical pharmacology, 18(6), 1307–1313. [Content Brief]

[3]. Byrd, W. J., et al., (1977). Synthetic polyamines added to cultures containing bovine sera reversibly inhibit in vitro parameters of immunity. Nature, 267(5612), 621–623. [Content Brief]

[4]. Løvaas, E., & Carlin, G. (1991). Spermine: an anti-oxidant and anti-inflammatory agent. Free radical biology & medicine, 11(5), 455–461. [Content Brief]

[5]. WANG Jing, et al., (2022) Preliminary Study on Spermine Tetrahydrochloride Inhibition of Platelet Activation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(3): 315-319.

[6]. Otsuki, M., et al., (1995). In vivo pharmacological study of spermine-induced neurotoxicity. Neuroscience letters, 196(1-2), 81–84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.9422 mL	24.7109 mL	49.4218 mL	123.5544 mL
	5 mM	0.9884 mL	4.9422 mL	9.8844 mL	24.7109 mL
	10 mM	0.4942 mL	2.4711 mL	4.9422 mL	12.3554 mL
	15 mM	0.3295 mL	1.6474 mL	3.2948 mL	8.2370 mL
	20 mM	0.2471 mL	1.2355 mL	2.4711 mL	6.1777 mL
	25 mM	0.1977 mL	0.9884 mL	1.9769 mL	4.9422 mL
	30 mM	0.1647 mL	0.8237 mL	1.6474 mL	4.1185 mL
	40 mM	0.1236 mL	0.6178 mL	1.2355 mL	3.0889 mL
	50 mM	0.0988 mL	0.4942 mL	0.9884 mL	2.4711 mL
	60 mM	0.0824 mL	0.4118 mL	0.8237 mL	2.0592 mL
	80 mM	0.0618 mL	0.3089 mL	0.6178 mL	1.5444 mL
	100 mM	0.0494 mL	0.2471 mL	0.4942 mL	1.2355 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Spermine Related Classifications

Neurological Disease Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spermine71-44-3NSC 268508 NeuridineNSC268508NSC-268508Endogenous MetaboliteBacterialReactive Oxygen Species (ROS)DNA/RNA Synthesispolyamine nitric oxide donorplateletantioxidantanti-inflammatoryneurotoxicityInhibitorinhibitorinhibit

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Spermine (Synonyms: NSC 268508; Neuridine)

Customer Review

Other Forms of Spermine:

Spermine Related Antibodies

8 Publications Citing Use of MCE Spermine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Spermine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Spermine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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